The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>

Olfaction is crucial in mediating various behaviors of social insects such as red imported fire ants, <i>Solenopsis invicta</i> Buren. Olfactory receptor (OR) complexes consist of odor-specific ORs and OR co-receptors (Orco). Orcos are highly conserved across insect taxa and are widely c...

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Autores principales:	Yuzhe Du, Jian Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	red imported fire ants <i>Solenopsis invicta</i> odor-binding proteins (OBPs) RNA interference (RNAi) electroantennography (EAG) qRT-PCR Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fc49df1bfa7e4aedbb2233e143b0cda3
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spelling	oai:doaj.org-article:fc49df1bfa7e4aedbb2233e143b0cda32021-11-25T16:52:37ZThe Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>10.3390/biom111115952218-273Xhttps://doaj.org/article/fc49df1bfa7e4aedbb2233e143b0cda32021-10-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1595https://doaj.org/toc/2218-273XOlfaction is crucial in mediating various behaviors of social insects such as red imported fire ants, <i>Solenopsis invicta</i> Buren. Olfactory receptor (OR) complexes consist of odor-specific ORs and OR co-receptors (Orco). Orcos are highly conserved across insect taxa and are widely co-expressed with ORs. Odorant binding proteins (OBPs) can transport semiochemicals to ORs as protein carriers and thus constitute the first molecular recognition step in insect olfaction. In this study, three OBP genes highly expressed in <i>S. invicta</i> antenna, OBP1, OBP5, OBP6, and Orco were partially silenced using RNA interference (RNAi). RNAi SiOBP5- and Orco-injected ants showed significantly lower EAG (electroantennography) responses to fire ant alarm pheromones and the alkaloid, 2,4,6-trimethylpyridine than water- or GFP-injected ants 72 h post injection. Subsequent qRT-PCR analysis demonstrated that the transcript level of the OBP1, OBP5, OBP6, and Orco significantly decreased 72 h after ants were injected with dsRNAs; however, there were no transcript level or EAG changes in ants fed dsRNAs. Our results suggest that <i>S. invicta</i> Orco and SiOBP5 are crucial to fire ants for their responses to alarm pheromones. RNAi knocking down SiOBP5 can significantly disrupt alarm pheromone communication, suggesting that disrupting SiOBP5 and Orcos could be potentially useful in the management of red imported fire ants.Yuzhe DuJian ChenMDPI AGarticlered imported fire ants<i>Solenopsis invicta</i>odor-binding proteins (OBPs)RNA interference (RNAi)electroantennography (EAG)qRT-PCRMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1595, p 1595 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	red imported fire ants <i>Solenopsis invicta</i> odor-binding proteins (OBPs) RNA interference (RNAi) electroantennography (EAG) qRT-PCR Microbiology QR1-502
spellingShingle	red imported fire ants <i>Solenopsis invicta</i> odor-binding proteins (OBPs) RNA interference (RNAi) electroantennography (EAG) qRT-PCR Microbiology QR1-502 Yuzhe Du Jian Chen The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>
description	Olfaction is crucial in mediating various behaviors of social insects such as red imported fire ants, <i>Solenopsis invicta</i> Buren. Olfactory receptor (OR) complexes consist of odor-specific ORs and OR co-receptors (Orco). Orcos are highly conserved across insect taxa and are widely co-expressed with ORs. Odorant binding proteins (OBPs) can transport semiochemicals to ORs as protein carriers and thus constitute the first molecular recognition step in insect olfaction. In this study, three OBP genes highly expressed in <i>S. invicta</i> antenna, OBP1, OBP5, OBP6, and Orco were partially silenced using RNA interference (RNAi). RNAi SiOBP5- and Orco-injected ants showed significantly lower EAG (electroantennography) responses to fire ant alarm pheromones and the alkaloid, 2,4,6-trimethylpyridine than water- or GFP-injected ants 72 h post injection. Subsequent qRT-PCR analysis demonstrated that the transcript level of the OBP1, OBP5, OBP6, and Orco significantly decreased 72 h after ants were injected with dsRNAs; however, there were no transcript level or EAG changes in ants fed dsRNAs. Our results suggest that <i>S. invicta</i> Orco and SiOBP5 are crucial to fire ants for their responses to alarm pheromones. RNAi knocking down SiOBP5 can significantly disrupt alarm pheromone communication, suggesting that disrupting SiOBP5 and Orcos could be potentially useful in the management of red imported fire ants.
format	article
author	Yuzhe Du Jian Chen
author_facet	Yuzhe Du Jian Chen
author_sort	Yuzhe Du
title	The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>
title_short	The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>
title_full	The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>
title_fullStr	The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>
title_full_unstemmed	The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>
title_sort	odorant binding protein, siobp5, mediates alarm pheromone olfactory recognition in the red imported fire ant, <i>solenopsis invicta</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fc49df1bfa7e4aedbb2233e143b0cda3
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The Odorant Binding Protein, SiOBP5, Mediates Alarm Pheromone Olfactory Recognition in the Red Imported Fire Ant, <i>Solenopsis invicta</i>

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