miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation

microRNAs (miRNAs), through their regulation of the expression and activity of numerous proteins, are involved in almost all cellular processes. As a consequence, dysregulation of miRNA expression is closely associated with the development and progression of cancers. Recently, DNA methylation has be...

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Autores principales:	Haibo Jiang, Ruixiang Ge, Siwen Chen, Laiquan Huang, Jie Mao, Lili Sheng
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	mirna-204-5p astrocytoma dna methylation ezrin invasion and migration Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/fc4e54b601fe4d5ea60a85a3b187db4d
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spelling	oai:doaj.org-article:fc4e54b601fe4d5ea60a85a3b187db4d2021-11-26T11:19:49ZmiRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation2165-59792165-598710.1080/21655979.2021.2000244https://doaj.org/article/fc4e54b601fe4d5ea60a85a3b187db4d2021-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2000244https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987microRNAs (miRNAs), through their regulation of the expression and activity of numerous proteins, are involved in almost all cellular processes. As a consequence, dysregulation of miRNA expression is closely associated with the development and progression of cancers. Recently, DNA methylation has been shown to play a key role in miRNA expression dysregulation in tumors. miRNA-204-5p commonly acts in the suppression of oncogenes in tumors. In this study, the levels of miRNA-204-5p were found to be down-regulated in the astrocytoma samples. miRNA-204-5p expression was also down-regulated in two astrocytoma cell lines (U87MG and LN382). Examination of online databases showed that the miRNA-204-5p promoter regions exist in CpG islands, which might be subjected to differential methylation. Subsequently, we showed that the miRNA-204-5p promoter region was hypermethylated in the astrocytoma tissue samples and cell lines. Then we found that ezrin expression was down-regulated with an increase in miRNA-204-5p expression in LN382 and U87MG cells after 5-aza-2ʹ-deoxycytidine (5ʹAZA) treatment compared with control DMSO treatment. In addition, LN382 and U87MG cells treated with 5ʹAZA exhibited significantly inhibited cell invasion and migration . In a recovery experiment, cell invasion and migration returned to normal levels as miRNA-204-5p and ezrin levels were restored. Overall, our study suggests that miRNA-204-5p acts as a tumor suppressor to influence astrocytoma invasion and migration by targeting ezrin and that miRNA-204-5p expression is downregulated by DNA methylation. This study provides a new potential strategy for astrocytoma treatment.Haibo JiangRuixiang GeSiwen ChenLaiquan HuangJie MaoLili ShengTaylor & Francis Grouparticlemirna-204-5pastrocytomadna methylationezrininvasion and migrationBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 2, Pp 9301-9312 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mirna-204-5p astrocytoma dna methylation ezrin invasion and migration Biotechnology TP248.13-248.65
spellingShingle	mirna-204-5p astrocytoma dna methylation ezrin invasion and migration Biotechnology TP248.13-248.65 Haibo Jiang Ruixiang Ge Siwen Chen Laiquan Huang Jie Mao Lili Sheng miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation
description	microRNAs (miRNAs), through their regulation of the expression and activity of numerous proteins, are involved in almost all cellular processes. As a consequence, dysregulation of miRNA expression is closely associated with the development and progression of cancers. Recently, DNA methylation has been shown to play a key role in miRNA expression dysregulation in tumors. miRNA-204-5p commonly acts in the suppression of oncogenes in tumors. In this study, the levels of miRNA-204-5p were found to be down-regulated in the astrocytoma samples. miRNA-204-5p expression was also down-regulated in two astrocytoma cell lines (U87MG and LN382). Examination of online databases showed that the miRNA-204-5p promoter regions exist in CpG islands, which might be subjected to differential methylation. Subsequently, we showed that the miRNA-204-5p promoter region was hypermethylated in the astrocytoma tissue samples and cell lines. Then we found that ezrin expression was down-regulated with an increase in miRNA-204-5p expression in LN382 and U87MG cells after 5-aza-2ʹ-deoxycytidine (5ʹAZA) treatment compared with control DMSO treatment. In addition, LN382 and U87MG cells treated with 5ʹAZA exhibited significantly inhibited cell invasion and migration . In a recovery experiment, cell invasion and migration returned to normal levels as miRNA-204-5p and ezrin levels were restored. Overall, our study suggests that miRNA-204-5p acts as a tumor suppressor to influence astrocytoma invasion and migration by targeting ezrin and that miRNA-204-5p expression is downregulated by DNA methylation. This study provides a new potential strategy for astrocytoma treatment.
format	article
author	Haibo Jiang Ruixiang Ge Siwen Chen Laiquan Huang Jie Mao Lili Sheng
author_facet	Haibo Jiang Ruixiang Ge Siwen Chen Laiquan Huang Jie Mao Lili Sheng
author_sort	Haibo Jiang
title	miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation
title_short	miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation
title_full	miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation
title_fullStr	miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation
title_full_unstemmed	miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation
title_sort	mirna-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by dna methylation
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/fc4e54b601fe4d5ea60a85a3b187db4d
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miRNA-204-5p acts as tumor suppressor to influence the invasion and migration of astrocytoma by targeting ezrin and is downregulated by DNA methylation

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