Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and removed using the GuideScan sgRNA specificity score.

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Bibliographic Details
Main Authors: Josh Tycko, Michael Wainberg, Georgi K. Marinov, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa Truong, Alexandro E. Trevino, Kaitlyn Spees, David Yao, Irene M. Kaplow, Peyton G. Greenside, David W. Morgens, Douglas H. Phanstiel, Michael P. Snyder, Lacramioara Bintu, William J. Greenleaf, Anshul Kundaje, Michael C. Bassik
Format: article
Language:EN
Published: Nature Portfolio 2019
Subjects:
Science
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Online Access:https://doaj.org/article/fc5611c303ab470e9fff314f00d1301c
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Summary:Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and removed using the GuideScan sgRNA specificity score.

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