Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and removed using the GuideScan sgRNA specificity score.

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Autores principales:	Josh Tycko, Michael Wainberg, Georgi K. Marinov, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa Truong, Alexandro E. Trevino, Kaitlyn Spees, David Yao, Irene M. Kaplow, Peyton G. Greenside, David W. Morgens, Douglas H. Phanstiel, Michael P. Snyder, Lacramioara Bintu, William J. Greenleaf, Anshul Kundaje, Michael C. Bassik
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/fc5611c303ab470e9fff314f00d1301c
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spelling	oai:doaj.org-article:fc5611c303ab470e9fff314f00d1301c2021-12-02T16:58:06ZMitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements10.1038/s41467-019-11955-72041-1723https://doaj.org/article/fc5611c303ab470e9fff314f00d1301c2019-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11955-7https://doaj.org/toc/2041-1723Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and removed using the GuideScan sgRNA specificity score.Josh TyckoMichael WainbergGeorgi K. MarinovOana UrsuGaelen T. HessBraeden K. EgoAradhanaAmy LiAlisa TruongAlexandro E. TrevinoKaitlyn SpeesDavid YaoIrene M. KaplowPeyton G. GreensideDavid W. MorgensDouglas H. PhanstielMichael P. SnyderLacramioara BintuWilliam J. GreenleafAnshul KundajeMichael C. BassikNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Josh Tycko Michael Wainberg Georgi K. Marinov Oana Ursu Gaelen T. Hess Braeden K. Ego Aradhana Amy Li Alisa Truong Alexandro E. Trevino Kaitlyn Spees David Yao Irene M. Kaplow Peyton G. Greenside David W. Morgens Douglas H. Phanstiel Michael P. Snyder Lacramioara Bintu William J. Greenleaf Anshul Kundaje Michael C. Bassik Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
description	Off-target effects in CRISPR screens for essential regulatory elements have not been systematically evaluated. Here the authors find Cas9 nuclease, CRISPRi/a each have distinct off-target effects, and that these can be accurately identified and removed using the GuideScan sgRNA specificity score.
format	article
author	Josh Tycko Michael Wainberg Georgi K. Marinov Oana Ursu Gaelen T. Hess Braeden K. Ego Aradhana Amy Li Alisa Truong Alexandro E. Trevino Kaitlyn Spees David Yao Irene M. Kaplow Peyton G. Greenside David W. Morgens Douglas H. Phanstiel Michael P. Snyder Lacramioara Bintu William J. Greenleaf Anshul Kundaje Michael C. Bassik
author_facet	Josh Tycko Michael Wainberg Georgi K. Marinov Oana Ursu Gaelen T. Hess Braeden K. Ego Aradhana Amy Li Alisa Truong Alexandro E. Trevino Kaitlyn Spees David Yao Irene M. Kaplow Peyton G. Greenside David W. Morgens Douglas H. Phanstiel Michael P. Snyder Lacramioara Bintu William J. Greenleaf Anshul Kundaje Michael C. Bassik
author_sort	Josh Tycko
title	Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
title_short	Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
title_full	Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
title_fullStr	Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
title_full_unstemmed	Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
title_sort	mitigation of off-target toxicity in crispr-cas9 screens for essential non-coding elements
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/fc5611c303ab470e9fff314f00d1301c
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Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements

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