Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats

Antenatal corticosteroid therapy is used to reduce neonatal mortality in preterm infants but it is currently unknown whether this intervention affects lipid metabolism at the peripartum. This study aimed to evaluate if antenatal corticosteroid therapy in pregnant rats and women affects lipid metabol...

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Autores principales:	Fernanda Ballerini Hecht, Caio Jordão Teixeira, Dailson Nogueira de Souza, Filiphe de Paula Nunes Mesquita, Ryana Elyzabeth do Val Roso, Frhancielly Shirley Sodré, Vanessa Barbosa Veronesi, Deborah Fabiana da Rocha, Yan Guida Dantas de Menezes, Mariana Rodrigues Pioli, Silvana Bordin, José Guilherme Cecatti, Adriana Gomes Luz, Gabriel Forato Anhê
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Glucocorticoids Pregnancy Lactation Lipid metabolism Liver Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/fc58fab1ce394e9cbb7ee8ded715c332
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spelling	oai:doaj.org-article:fc58fab1ce394e9cbb7ee8ded715c3322021-11-14T04:30:14ZAntenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats0753-332210.1016/j.biopha.2021.112355https://doaj.org/article/fc58fab1ce394e9cbb7ee8ded715c3322021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011392https://doaj.org/toc/0753-3322Antenatal corticosteroid therapy is used to reduce neonatal mortality in preterm infants but it is currently unknown whether this intervention affects lipid metabolism at the peripartum. This study aimed to evaluate if antenatal corticosteroid therapy in pregnant rats and women affects lipid metabolism during early lactation. We evaluated women at risk of preterm delivery that received corticosteroid therapy (CASE) and women that were not exposed to corticosteroid and were not at risk of preterm delivery (CONTROL). Samples were collected to measure serum and milk triacylglycerol (TAG) three days after delivery. Rats were treated with dexamethasone (DEX) between the 15th and the 20th days of pregnancy. Samples were collected at different days after delivery (L3, L8 and L14). TAG was measured in serum, liver and mammary gland (MG). TAG appearance rates were measured after tyloxapol injection and gavage with olive oil. We also evaluated the expression of key genes related to lipid metabolism in the liver and in the MG and hepatic phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC). CASE volunteers delivered earlier than CONTROL but presented unaltered milk and serum TAG concentrations. Early lactating DEX rats exhibited increased TAG in serum, MG and milk. No changes in CD36 and LPL were detected in the MG and liver. Early lactating DEX rats displayed increased TAG appearance rate and reduced hepatic AMPK/ACC phosphorylation. Our data revealed that antenatal corticosteroid therapy reduces hepatic AMPK/ACC phosphorylation during early lactation that reflects in increased TAG concentration in serum, MG and milk.Fernanda Ballerini HechtCaio Jordão TeixeiraDailson Nogueira de SouzaFiliphe de Paula Nunes MesquitaRyana Elyzabeth do Val RosoFrhancielly Shirley SodréVanessa Barbosa VeronesiDeborah Fabiana da RochaYan Guida Dantas de MenezesMariana Rodrigues PioliSilvana BordinJosé Guilherme CecattiAdriana Gomes LuzGabriel Forato AnhêElsevierarticleGlucocorticoidsPregnancyLactationLipid metabolismLiverTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112355- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Glucocorticoids Pregnancy Lactation Lipid metabolism Liver Therapeutics. Pharmacology RM1-950
spellingShingle	Glucocorticoids Pregnancy Lactation Lipid metabolism Liver Therapeutics. Pharmacology RM1-950 Fernanda Ballerini Hecht Caio Jordão Teixeira Dailson Nogueira de Souza Filiphe de Paula Nunes Mesquita Ryana Elyzabeth do Val Roso Frhancielly Shirley Sodré Vanessa Barbosa Veronesi Deborah Fabiana da Rocha Yan Guida Dantas de Menezes Mariana Rodrigues Pioli Silvana Bordin José Guilherme Cecatti Adriana Gomes Luz Gabriel Forato Anhê Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats
description	Antenatal corticosteroid therapy is used to reduce neonatal mortality in preterm infants but it is currently unknown whether this intervention affects lipid metabolism at the peripartum. This study aimed to evaluate if antenatal corticosteroid therapy in pregnant rats and women affects lipid metabolism during early lactation. We evaluated women at risk of preterm delivery that received corticosteroid therapy (CASE) and women that were not exposed to corticosteroid and were not at risk of preterm delivery (CONTROL). Samples were collected to measure serum and milk triacylglycerol (TAG) three days after delivery. Rats were treated with dexamethasone (DEX) between the 15th and the 20th days of pregnancy. Samples were collected at different days after delivery (L3, L8 and L14). TAG was measured in serum, liver and mammary gland (MG). TAG appearance rates were measured after tyloxapol injection and gavage with olive oil. We also evaluated the expression of key genes related to lipid metabolism in the liver and in the MG and hepatic phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC). CASE volunteers delivered earlier than CONTROL but presented unaltered milk and serum TAG concentrations. Early lactating DEX rats exhibited increased TAG in serum, MG and milk. No changes in CD36 and LPL were detected in the MG and liver. Early lactating DEX rats displayed increased TAG appearance rate and reduced hepatic AMPK/ACC phosphorylation. Our data revealed that antenatal corticosteroid therapy reduces hepatic AMPK/ACC phosphorylation during early lactation that reflects in increased TAG concentration in serum, MG and milk.
format	article
author	Fernanda Ballerini Hecht Caio Jordão Teixeira Dailson Nogueira de Souza Filiphe de Paula Nunes Mesquita Ryana Elyzabeth do Val Roso Frhancielly Shirley Sodré Vanessa Barbosa Veronesi Deborah Fabiana da Rocha Yan Guida Dantas de Menezes Mariana Rodrigues Pioli Silvana Bordin José Guilherme Cecatti Adriana Gomes Luz Gabriel Forato Anhê
author_facet	Fernanda Ballerini Hecht Caio Jordão Teixeira Dailson Nogueira de Souza Filiphe de Paula Nunes Mesquita Ryana Elyzabeth do Val Roso Frhancielly Shirley Sodré Vanessa Barbosa Veronesi Deborah Fabiana da Rocha Yan Guida Dantas de Menezes Mariana Rodrigues Pioli Silvana Bordin José Guilherme Cecatti Adriana Gomes Luz Gabriel Forato Anhê
author_sort	Fernanda Ballerini Hecht
title	Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats
title_short	Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats
title_full	Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats
title_fullStr	Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats
title_full_unstemmed	Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats
title_sort	antenatal corticosteroid therapy modulates hepatic ampk phosphorylation and maternal lipid metabolism in early lactating rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/fc58fab1ce394e9cbb7ee8ded715c332
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Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats

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