KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion

Background. Pancreatic cancer is a malignant tumor of the digestive tract, which is difficult to diagnose and treat due to bad early diagnosis. We aimed to explore the role of kinesin superfamily 4A (KIF4A) in pancreatic ductal adenocarcinoma (PDAC). Methods. We first used the bioinformatic website...

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Autores principales:	Jing Chen, Cui-Cui Zhao, Fei-Ran Chen, Guo-Wei Feng, Fei Luo, Tao Jiang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Medicine R
Acceso en línea:	https://doaj.org/article/fc6290cee12149eabfd5c9635502ad74
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spelling	oai:doaj.org-article:fc6290cee12149eabfd5c9635502ad742021-11-22T01:09:44ZKIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion2314-614110.1155/2021/8249293https://doaj.org/article/fc6290cee12149eabfd5c9635502ad742021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/8249293https://doaj.org/toc/2314-6141Background. Pancreatic cancer is a malignant tumor of the digestive tract, which is difficult to diagnose and treat due to bad early diagnosis. We aimed to explore the role of kinesin superfamily 4A (KIF4A) in pancreatic ductal adenocarcinoma (PDAC). Methods. We first used the bioinformatic website to screen the data of pancreatic cancer in TCGA, and KIF4A protein was detected among the 86 specimens of patients in our hospital combined with clinic-pathological characteristics and survival analysis. KIF4A loss-expression cell lines were established by RNA interference (RNAi). In addition, we performed in vitro cell assays to detect the changes in cell proliferation, migration, and invasion. The proteins involved in the proliferation and metastasis of cancer cells were also detected by western blot. The above results could be proved in vivo. Further, the correlation between KIF4A and CDC5L was analyzed by TCGA and IHC data. Results. We first found a high expression of KIF4A in pancreatic cancer, suggesting a role of KIF4A in the development of pancreatic cancer. KIF4A was found to be differentially expressed (P<0.05) among the 86 specimens of patients in our hospital and was significantly associated with PDAC TNM stages and tumor size. High KIF4A expression also significantly worsened overall survival (OS) and disease-free survival rate (DFS) (P<0.05, respectively). In addition, cell proliferation, migration, and invasion were inhibited by the KIF4A-shRNA group compared with the control (P<0.05, respectively). In the end, knockdown of KIF4A could inhibit tumor development and metastasis in vivo. Further, the positive correlation between KIF4A and CDC5L existed, and KIF4A might promote pancreatic cancer proliferation by affecting CDC5L expression. Conclusion. In conclusion, the high expression level of KIF4A in PDAC was closely related to poor clinical and pathological status, lymphatic metastasis, and vascular invasion. KIF4A might be involved in promoting the development of PDAC in vitro and in vivo, which might be a new therapeutic target of PDAC.Jing ChenCui-Cui ZhaoFei-Ran ChenGuo-Wei FengFei LuoTao JiangHindawi LimitedarticleMedicineRENBioMed Research International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R
spellingShingle	Medicine R Jing Chen Cui-Cui Zhao Fei-Ran Chen Guo-Wei Feng Fei Luo Tao Jiang KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion
description	Background. Pancreatic cancer is a malignant tumor of the digestive tract, which is difficult to diagnose and treat due to bad early diagnosis. We aimed to explore the role of kinesin superfamily 4A (KIF4A) in pancreatic ductal adenocarcinoma (PDAC). Methods. We first used the bioinformatic website to screen the data of pancreatic cancer in TCGA, and KIF4A protein was detected among the 86 specimens of patients in our hospital combined with clinic-pathological characteristics and survival analysis. KIF4A loss-expression cell lines were established by RNA interference (RNAi). In addition, we performed in vitro cell assays to detect the changes in cell proliferation, migration, and invasion. The proteins involved in the proliferation and metastasis of cancer cells were also detected by western blot. The above results could be proved in vivo. Further, the correlation between KIF4A and CDC5L was analyzed by TCGA and IHC data. Results. We first found a high expression of KIF4A in pancreatic cancer, suggesting a role of KIF4A in the development of pancreatic cancer. KIF4A was found to be differentially expressed (P<0.05) among the 86 specimens of patients in our hospital and was significantly associated with PDAC TNM stages and tumor size. High KIF4A expression also significantly worsened overall survival (OS) and disease-free survival rate (DFS) (P<0.05, respectively). In addition, cell proliferation, migration, and invasion were inhibited by the KIF4A-shRNA group compared with the control (P<0.05, respectively). In the end, knockdown of KIF4A could inhibit tumor development and metastasis in vivo. Further, the positive correlation between KIF4A and CDC5L existed, and KIF4A might promote pancreatic cancer proliferation by affecting CDC5L expression. Conclusion. In conclusion, the high expression level of KIF4A in PDAC was closely related to poor clinical and pathological status, lymphatic metastasis, and vascular invasion. KIF4A might be involved in promoting the development of PDAC in vitro and in vivo, which might be a new therapeutic target of PDAC.
format	article
author	Jing Chen Cui-Cui Zhao Fei-Ran Chen Guo-Wei Feng Fei Luo Tao Jiang
author_facet	Jing Chen Cui-Cui Zhao Fei-Ran Chen Guo-Wei Feng Fei Luo Tao Jiang
author_sort	Jing Chen
title	KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion
title_short	KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion
title_full	KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion
title_fullStr	KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion
title_full_unstemmed	KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion
title_sort	kif4a regulates the progression of pancreatic ductal adenocarcinoma through proliferation and invasion
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/fc6290cee12149eabfd5c9635502ad74
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KIF4A Regulates the Progression of Pancreatic Ductal Adenocarcinoma through Proliferation and Invasion

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