Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.

Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by internal phosphoester transfer. Comparative sequence analysis was used to identify numerous additional representatives of this ribozyme class than were previously known, including the first representatives in fungi and...

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Autores principales:	Jonathan Perreault, Zasha Weinberg, Adam Roth, Olivia Popescu, Pascal Chartrand, Gerardo Ferbeyre, Ronald R Breaker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fc65e8886f0441448eb765d8ca06952b
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spelling	oai:doaj.org-article:fc65e8886f0441448eb765d8ca06952b2021-11-18T05:50:33ZIdentification of hammerhead ribozymes in all domains of life reveals novel structural variations.1553-734X1553-735810.1371/journal.pcbi.1002031https://doaj.org/article/fc65e8886f0441448eb765d8ca06952b2011-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21573207/pdf/?tool=EBIhttps://doaj.org/toc/1553-734Xhttps://doaj.org/toc/1553-7358Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by internal phosphoester transfer. Comparative sequence analysis was used to identify numerous additional representatives of this ribozyme class than were previously known, including the first representatives in fungi and archaea. Moreover, we have uncovered the first natural examples of "type II" hammerheads, and our findings reveal that this permuted form occurs in bacteria as frequently as type I and III architectures. We also identified a commonly occurring pseudoknot that forms a tertiary interaction critical for high-speed ribozyme activity. Genomic contexts of many hammerhead ribozymes indicate that they perform biological functions different from their known role in generating unit-length RNA transcripts of multimeric viroid and satellite virus genomes. In rare instances, nucleotide variation occurs at positions within the catalytic core that are otherwise strictly conserved, suggesting that core mutations are occasionally tolerated or preferred.Jonathan PerreaultZasha WeinbergAdam RothOlivia PopescuPascal ChartrandGerardo FerbeyreRonald R BreakerPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Computational Biology, Vol 7, Iss 5, p e1002031 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Jonathan Perreault Zasha Weinberg Adam Roth Olivia Popescu Pascal Chartrand Gerardo Ferbeyre Ronald R Breaker Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.
description	Hammerhead ribozymes are small self-cleaving RNAs that promote strand scission by internal phosphoester transfer. Comparative sequence analysis was used to identify numerous additional representatives of this ribozyme class than were previously known, including the first representatives in fungi and archaea. Moreover, we have uncovered the first natural examples of "type II" hammerheads, and our findings reveal that this permuted form occurs in bacteria as frequently as type I and III architectures. We also identified a commonly occurring pseudoknot that forms a tertiary interaction critical for high-speed ribozyme activity. Genomic contexts of many hammerhead ribozymes indicate that they perform biological functions different from their known role in generating unit-length RNA transcripts of multimeric viroid and satellite virus genomes. In rare instances, nucleotide variation occurs at positions within the catalytic core that are otherwise strictly conserved, suggesting that core mutations are occasionally tolerated or preferred.
format	article
author	Jonathan Perreault Zasha Weinberg Adam Roth Olivia Popescu Pascal Chartrand Gerardo Ferbeyre Ronald R Breaker
author_facet	Jonathan Perreault Zasha Weinberg Adam Roth Olivia Popescu Pascal Chartrand Gerardo Ferbeyre Ronald R Breaker
author_sort	Jonathan Perreault
title	Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.
title_short	Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.
title_full	Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.
title_fullStr	Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.
title_full_unstemmed	Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.
title_sort	identification of hammerhead ribozymes in all domains of life reveals novel structural variations.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fc65e8886f0441448eb765d8ca06952b
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Identification of hammerhead ribozymes in all domains of life reveals novel structural variations.

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