A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates

This authentic research project is designed to identify environmental isolates by metabolic phenotypes and 16s sequence analysis and with an investigation of biofilm growth is presented as implemented in an upper-level microbiology lab course. Three units were used in the lab: one for basic metaboli...

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Autor principal:	Christa Chatfield
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fc677f8cfbae45c7a014a2362a4289b3
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spelling	oai:doaj.org-article:fc677f8cfbae45c7a014a2362a4289b32021-11-15T15:15:37ZA Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates10.1128/jmbe.v15i2.7891935-78851935-7877https://doaj.org/article/fc677f8cfbae45c7a014a2362a4289b32014-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v15i2.789https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885This authentic research project is designed to identify environmental isolates by metabolic phenotypes and 16s sequence analysis and with an investigation of biofilm growth is presented as implemented in an upper-level microbiology lab course. Three units were used in the lab: one for basic metabolic identification, one for the 16s rDNA sequencing and a third for biofilm growth analysis. Assessment was by weekly notebook entries detailing the outcomes of each day in lab, providing relatively on-time feedback on student understanding and learning to both the student and the instructor. The intent for these units was for each to increase the uncertainty of the project outcomes and to challenge students to design projects with open-ended results. All student groups have been able to obtain DNA sequence data in the limited 6-7 weeks of the lab project. Students report increased confidence in their abilities and a general excitement about the project methods and results. The data produced by the students can be incorporated into larger research questions posed by the faculty running the course as determined by the source of the unknown bacterial isolates.Christa ChatfieldAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 15, Iss 2, Pp 325-327 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Christa Chatfield A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates
description	This authentic research project is designed to identify environmental isolates by metabolic phenotypes and 16s sequence analysis and with an investigation of biofilm growth is presented as implemented in an upper-level microbiology lab course. Three units were used in the lab: one for basic metabolic identification, one for the 16s rDNA sequencing and a third for biofilm growth analysis. Assessment was by weekly notebook entries detailing the outcomes of each day in lab, providing relatively on-time feedback on student understanding and learning to both the student and the instructor. The intent for these units was for each to increase the uncertainty of the project outcomes and to challenge students to design projects with open-ended results. All student groups have been able to obtain DNA sequence data in the limited 6-7 weeks of the lab project. Students report increased confidence in their abilities and a general excitement about the project methods and results. The data produced by the students can be incorporated into larger research questions posed by the faculty running the course as determined by the source of the unknown bacterial isolates.
format	article
author	Christa Chatfield
author_facet	Christa Chatfield
author_sort	Christa Chatfield
title	A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates
title_short	A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates
title_full	A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates
title_fullStr	A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates
title_full_unstemmed	A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates
title_sort	multi-unit project for building scientific confidence via authentic research in identification of environmental bacterial isolates
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/fc677f8cfbae45c7a014a2362a4289b3
work_keys_str_mv	AT christachatfield amultiunitprojectforbuildingscientificconfidenceviaauthenticresearchinidentificationofenvironmentalbacterialisolates AT christachatfield multiunitprojectforbuildingscientificconfidenceviaauthenticresearchinidentificationofenvironmentalbacterialisolates
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A Multi-Unit Project for Building Scientific Confidence via Authentic Research in Identification of Environmental Bacterial Isolates

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