Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

Abstract This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were an...

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Autores principales:	Charmaine Lloyd, Kai Heng Tan, Kar Leong Lim, Vimala Gana Valu, Sarah Mei Ying Fun, Teng Rong Chye, Hui Min Mak, Wei Xiong Sim, Sarah Liyana Musa, Joscelyn Jun Quan Ng, Nazurah Syazana Bte Nordin, Nurhazlyn Bte Md Aidzil, Zephyr Yu Wen Eng, Punithavathy Manickavasagam, Jen Yan New
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc6a93cfa3144373a94a19881ebffff3
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spelling	oai:doaj.org-article:fc6a93cfa3144373a94a19881ebffff32021-12-02T15:53:02ZIdentification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city10.1038/s41598-021-84112-02045-2322https://doaj.org/article/fc6a93cfa3144373a94a19881ebffff32021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84112-0https://doaj.org/toc/2045-2322Abstract This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, EH = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.Charmaine LloydKai Heng TanKar Leong LimVimala Gana ValuSarah Mei Ying FunTeng Rong ChyeHui Min MakWei Xiong SimSarah Liyana MusaJoscelyn Jun Quan NgNazurah Syazana Bte NordinNurhazlyn Bte Md AidzilZephyr Yu Wen EngPunithavathy ManickavasagamJen Yan NewNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-6 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Charmaine Lloyd Kai Heng Tan Kar Leong Lim Vimala Gana Valu Sarah Mei Ying Fun Teng Rong Chye Hui Min Mak Wei Xiong Sim Sarah Liyana Musa Joscelyn Jun Quan Ng Nazurah Syazana Bte Nordin Nurhazlyn Bte Md Aidzil Zephyr Yu Wen Eng Punithavathy Manickavasagam Jen Yan New Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
description	Abstract This study aimed at exploring microalgal heterogeneity from fresh water samples collected from inland water bodies in the heavily built city of Singapore. Culturable pure isolates (n = 94) were subject to an in-house microalgal DNA extraction method and LSU rDNA sequencing. Isolates were analysed for their predominance and distribution. A total of 17 different algal genera were identified (H = 2.8, EH = 0.6), of which Scenedesmus spp. and Chlorella spp. constituted 27.5% and 21.3% of isolates respectively, followed by Micractinium spp. (18.8%) and Chlamydomonas spp. (12.5%). We also report 16 new microalgal strains from this region. The data is important from an ecological and biotechnological perspective.
format	article
author	Charmaine Lloyd Kai Heng Tan Kar Leong Lim Vimala Gana Valu Sarah Mei Ying Fun Teng Rong Chye Hui Min Mak Wei Xiong Sim Sarah Liyana Musa Joscelyn Jun Quan Ng Nazurah Syazana Bte Nordin Nurhazlyn Bte Md Aidzil Zephyr Yu Wen Eng Punithavathy Manickavasagam Jen Yan New
author_facet	Charmaine Lloyd Kai Heng Tan Kar Leong Lim Vimala Gana Valu Sarah Mei Ying Fun Teng Rong Chye Hui Min Mak Wei Xiong Sim Sarah Liyana Musa Joscelyn Jun Quan Ng Nazurah Syazana Bte Nordin Nurhazlyn Bte Md Aidzil Zephyr Yu Wen Eng Punithavathy Manickavasagam Jen Yan New
author_sort	Charmaine Lloyd
title	Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_short	Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_full	Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_fullStr	Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_full_unstemmed	Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city
title_sort	identification of microalgae cultured in bold’s basal medium from freshwater samples, from a high-rise city
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fc6a93cfa3144373a94a19881ebffff3
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Identification of microalgae cultured in Bold’s Basal medium from freshwater samples, from a high-rise city

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