Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus

ABSTRACT Sialic acids (Sias) are important glycans displayed on the cells and tissues of many different animals and are frequent targets for binding and modification by pathogens, including influenza viruses. Influenza virus hemagglutinins bind Sias during the infection of their normal hosts, while...

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Autores principales:	Brian R. Wasik, Karen N. Barnard, Robert J. Ossiboff, Zahra Khedri, Kurtis H. Feng, Hai Yu, Xi Chen, Daniel R. Perez, Ajit Varki, Colin R. Parrish
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	host range influenza receptor-ligand interaction respiratory viruses sialic acid virus-host interactions Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fc6ce7f68e4b4ef8a04c462e9d7f2cac
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spelling	oai:doaj.org-article:fc6ce7f68e4b4ef8a04c462e9d7f2cac2021-11-15T15:22:05ZDistribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus10.1128/mSphere.00379-162379-5042https://doaj.org/article/fc6ce7f68e4b4ef8a04c462e9d7f2cac2017-10-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00379-16https://doaj.org/toc/2379-5042ABSTRACT Sialic acids (Sias) are important glycans displayed on the cells and tissues of many different animals and are frequent targets for binding and modification by pathogens, including influenza viruses. Influenza virus hemagglutinins bind Sias during the infection of their normal hosts, while the encoded neuraminidases and/or esterases remove or modify the Sia to allow virion release or to prevent rebinding. Sias naturally occur in a variety of modified forms, and modified Sias can alter influenza virus host tropisms through their altered interactions with the viral glycoproteins. However, the distribution of modified Sia forms and their effects on pathogen-host interactions are still poorly understood. Here we used probes developed from viral Sia-binding proteins to detect O-acetylated (4-O-acetyl, 9-O-acetyl, and 7,9-O-acetyl) Sias displayed on the tissues of some natural or experimental hosts for influenza viruses. These modified Sias showed highly variable displays between the hosts and tissues examined. The 9-O-acetyl (and 7,9-) modified Sia forms were found on cells and tissues of many hosts, including mice, humans, ferrets, guinea pigs, pigs, horses, dogs, as well as in those of ducks and embryonated chicken egg tissues and membranes, although in variable amounts. The 4-O-acetyl Sias were found in the respiratory tissues of fewer animals, being primarily displayed in the horse and guinea pig, but were not detected in humans or pigs. The results suggest that these Sia variants may influence virus tropisms by altering and selecting their cell interactions. IMPORTANCE Sialic acids (Sias) are key glycans that control or modulate many normal cell and tissue functions while also interacting with a variety of pathogens, including many different viruses. Sias are naturally displayed in a variety of different forms, with modifications at several positions that can alter their functional interactions with pathogens. In addition, Sias are often modified or removed by enzymes such as host or pathogen esterases or sialidases (neuraminidases), and Sia modifications can alter those enzymatic activities to impact pathogen infections. Sia chemical diversity in different hosts and tissues likely alters the pathogen-host interactions and influences the outcome of infection. Here we explored the display of 4-O-acetyl, 9-O-acetyl, and 7,9-O-acetyl modified Sia forms in some target tissues for influenza virus infection in mice, humans, birds, guinea pigs, ferrets, swine, horses, and dogs, which encompass many natural and laboratory hosts of those viruses.Brian R. WasikKaren N. BarnardRobert J. OssiboffZahra KhedriKurtis H. FengHai YuXi ChenDaniel R. PerezAjit VarkiColin R. ParrishAmerican Society for Microbiologyarticlehost rangeinfluenzareceptor-ligand interactionrespiratory virusessialic acidvirus-host interactionsMicrobiologyQR1-502ENmSphere, Vol 2, Iss 5 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	host range influenza receptor-ligand interaction respiratory viruses sialic acid virus-host interactions Microbiology QR1-502
spellingShingle	host range influenza receptor-ligand interaction respiratory viruses sialic acid virus-host interactions Microbiology QR1-502 Brian R. Wasik Karen N. Barnard Robert J. Ossiboff Zahra Khedri Kurtis H. Feng Hai Yu Xi Chen Daniel R. Perez Ajit Varki Colin R. Parrish Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus
description	ABSTRACT Sialic acids (Sias) are important glycans displayed on the cells and tissues of many different animals and are frequent targets for binding and modification by pathogens, including influenza viruses. Influenza virus hemagglutinins bind Sias during the infection of their normal hosts, while the encoded neuraminidases and/or esterases remove or modify the Sia to allow virion release or to prevent rebinding. Sias naturally occur in a variety of modified forms, and modified Sias can alter influenza virus host tropisms through their altered interactions with the viral glycoproteins. However, the distribution of modified Sia forms and their effects on pathogen-host interactions are still poorly understood. Here we used probes developed from viral Sia-binding proteins to detect O-acetylated (4-O-acetyl, 9-O-acetyl, and 7,9-O-acetyl) Sias displayed on the tissues of some natural or experimental hosts for influenza viruses. These modified Sias showed highly variable displays between the hosts and tissues examined. The 9-O-acetyl (and 7,9-) modified Sia forms were found on cells and tissues of many hosts, including mice, humans, ferrets, guinea pigs, pigs, horses, dogs, as well as in those of ducks and embryonated chicken egg tissues and membranes, although in variable amounts. The 4-O-acetyl Sias were found in the respiratory tissues of fewer animals, being primarily displayed in the horse and guinea pig, but were not detected in humans or pigs. The results suggest that these Sia variants may influence virus tropisms by altering and selecting their cell interactions. IMPORTANCE Sialic acids (Sias) are key glycans that control or modulate many normal cell and tissue functions while also interacting with a variety of pathogens, including many different viruses. Sias are naturally displayed in a variety of different forms, with modifications at several positions that can alter their functional interactions with pathogens. In addition, Sias are often modified or removed by enzymes such as host or pathogen esterases or sialidases (neuraminidases), and Sia modifications can alter those enzymatic activities to impact pathogen infections. Sia chemical diversity in different hosts and tissues likely alters the pathogen-host interactions and influences the outcome of infection. Here we explored the display of 4-O-acetyl, 9-O-acetyl, and 7,9-O-acetyl modified Sia forms in some target tissues for influenza virus infection in mice, humans, birds, guinea pigs, ferrets, swine, horses, and dogs, which encompass many natural and laboratory hosts of those viruses.
format	article
author	Brian R. Wasik Karen N. Barnard Robert J. Ossiboff Zahra Khedri Kurtis H. Feng Hai Yu Xi Chen Daniel R. Perez Ajit Varki Colin R. Parrish
author_facet	Brian R. Wasik Karen N. Barnard Robert J. Ossiboff Zahra Khedri Kurtis H. Feng Hai Yu Xi Chen Daniel R. Perez Ajit Varki Colin R. Parrish
author_sort	Brian R. Wasik
title	Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus
title_short	Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus
title_full	Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus
title_fullStr	Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus
title_full_unstemmed	Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus
title_sort	distribution of o-acetylated sialic acids among target host tissues for influenza virus
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/fc6ce7f68e4b4ef8a04c462e9d7f2cac
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Distribution of O-Acetylated Sialic Acids among Target Host Tissues for Influenza Virus

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