Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design

Luchen Zhang,1 Zhenbo Li,1 Fangli Sun,2 Yuhong Xu,1 Zixiu Du1 1School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 2School of Engineering, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang Province, People’s Republic of China Abstract: A safe and efficient...

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Autores principales:	Zhang L, Li Z, Sun F, Xu YH, Du Z
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Q-complexes spacer elements multifunctional peptides lipid component intracellular transfection Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fc732387dee04f4fa92758a837edae0b
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spelling	oai:doaj.org-article:fc732387dee04f4fa92758a837edae0b2021-12-02T07:45:45ZEffect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design1178-2013https://doaj.org/article/fc732387dee04f4fa92758a837edae0b2016-11-01T00:00:00Zhttps://www.dovepress.com/effect-of-inserted-spacer-in-hepatic-cell-penetrating-multifunctional--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Luchen Zhang,1 Zhenbo Li,1 Fangli Sun,2 Yuhong Xu,1 Zixiu Du1 1School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 2School of Engineering, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang Province, People’s Republic of China Abstract: A safe and efficient quaternary gene delivery system (named Q-complexes) was constructed based on self-assembly of molecules through noncovalent bonds. This system was formulated through the cooperation and competing interactions of cationic liposomes, multifunctional peptides, and DNA, followed by coating hyaluronic acid on the surface of the ternary complexes. The multifunctional peptide was composed of two functional domains: penetrating hepatic tumor-targeted cell moiety (KRPTMRFRYTWNPMK) and a wrapping gene sequence (polyarginine 16). The effect of spacer insertion between the two domains of multifunctional peptide on the intracellular transfection of Q-complexes was further studied. Experimental results showed that the formulations assembled with various peptides in the spacer elements possessed different intercellular pathways and transfection efficiencies. The Q-complexes containing peptide in the absence of spacer element (Pa) showed the highest gene expression among all samples. The Q-complexes containing peptides with a noncleavable spacer GA (Pc) had no ability of intracellular nucleic acid delivery, whereas those with a cleavable spacer RVRR (Pd) showed moderate transfection activity. These results demonstrated that the different spacers inserted in the multifunctional peptide played an important role in in vitro DNA transfection efficiency. Atomic force microscopy images showed that the morphologies of ternary complexes (LPcD) and Q-complexes (HLcPD) were crystal lamellas, whereas those of other nanocomplexes were spheres. Circular dichroism showed the changed configuration of peptide with spacer GA in nanocomplexes compared with that of its free state, whereas the Pa configuration without spacer in nanocomplexes was consistent with that of its free state. The present study contributed to the structural understanding of Q-complexes, and further effective modification is in progress. Keywords: Q-complexes, spacer elements, multifunctional peptides, lipid component, intracellular transfectionZhang LLi ZSun FXu YHDu ZDove Medical PressarticleQ-complexesspacer elementsmultifunctional peptideslipid componentintracellular transfectionMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 6283-6295 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Q-complexes spacer elements multifunctional peptides lipid component intracellular transfection Medicine (General) R5-920
spellingShingle	Q-complexes spacer elements multifunctional peptides lipid component intracellular transfection Medicine (General) R5-920 Zhang L Li Z Sun F Xu YH Du Z Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design
description	Luchen Zhang,1 Zhenbo Li,1 Fangli Sun,2 Yuhong Xu,1 Zixiu Du1 1School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 2School of Engineering, Zhejiang Agriculture and Forestry University, Lin’an, Zhejiang Province, People’s Republic of China Abstract: A safe and efficient quaternary gene delivery system (named Q-complexes) was constructed based on self-assembly of molecules through noncovalent bonds. This system was formulated through the cooperation and competing interactions of cationic liposomes, multifunctional peptides, and DNA, followed by coating hyaluronic acid on the surface of the ternary complexes. The multifunctional peptide was composed of two functional domains: penetrating hepatic tumor-targeted cell moiety (KRPTMRFRYTWNPMK) and a wrapping gene sequence (polyarginine 16). The effect of spacer insertion between the two domains of multifunctional peptide on the intracellular transfection of Q-complexes was further studied. Experimental results showed that the formulations assembled with various peptides in the spacer elements possessed different intercellular pathways and transfection efficiencies. The Q-complexes containing peptide in the absence of spacer element (Pa) showed the highest gene expression among all samples. The Q-complexes containing peptides with a noncleavable spacer GA (Pc) had no ability of intracellular nucleic acid delivery, whereas those with a cleavable spacer RVRR (Pd) showed moderate transfection activity. These results demonstrated that the different spacers inserted in the multifunctional peptide played an important role in in vitro DNA transfection efficiency. Atomic force microscopy images showed that the morphologies of ternary complexes (LPcD) and Q-complexes (HLcPD) were crystal lamellas, whereas those of other nanocomplexes were spheres. Circular dichroism showed the changed configuration of peptide with spacer GA in nanocomplexes compared with that of its free state, whereas the Pa configuration without spacer in nanocomplexes was consistent with that of its free state. The present study contributed to the structural understanding of Q-complexes, and further effective modification is in progress. Keywords: Q-complexes, spacer elements, multifunctional peptides, lipid component, intracellular transfection
format	article
author	Zhang L Li Z Sun F Xu YH Du Z
author_facet	Zhang L Li Z Sun F Xu YH Du Z
author_sort	Zhang L
title	Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design
title_short	Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design
title_full	Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design
title_fullStr	Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design
title_full_unstemmed	Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design
title_sort	effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the dna intracellular delivery of quaternary complexes based on modular design
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/fc732387dee04f4fa92758a837edae0b
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Effect of inserted spacer in hepatic cell-penetrating multifunctional peptide component on the DNA intracellular delivery of quaternary complexes based on modular design

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