Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay

In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for determination of thiamethoxam residues. We prepared a specific highly sensitive monoclonal antibody (mAb) based on a thiamethoxam-derived hapten. After mouse immunisation and cell fusion, we obtained...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liya Ye, Xiaoling Wu, Liguang Xu, Qiankun Zheng, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	thiamethoxam ic-elisa monoclonal antibody colloidal gold strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fc7e5e908c174bd28ccda83d161527d5
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fc7e5e908c174bd28ccda83d161527d5
record_format	dspace
spelling	oai:doaj.org-article:fc7e5e908c174bd28ccda83d161527d52021-11-26T11:19:47ZPreparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay0954-01051465-344310.1080/09540105.2018.1523373https://doaj.org/article/fc7e5e908c174bd28ccda83d161527d52018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1523373https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for determination of thiamethoxam residues. We prepared a specific highly sensitive monoclonal antibody (mAb) based on a thiamethoxam-derived hapten. After mouse immunisation and cell fusion, we obtained three monoclonal cell lines: 3B2, 3G6, and 4D2. The antibodies produced by cell line 4D2 had the highest affinity and sensitivity. The half-maximal inhibitory concentration was 0.87 ng/mL, and the cross-reactivity was <1%. We optimised the ic-ELISA method and the optimal conditions for ic-ELISA consisted of pH 7.4, 0.8% NaCl, and 0% methanol in PBS buffer. Using this mAb, an ic-ELISA and immunochromatographic strip (ICS) assay were used to detect thiamethoxam residues in cucumbers. The working range of ic-ELISA was 0.5–1.7 ng/mL, and the cut-off vaule of the ICS was 10 ng/mL. The recovery of thiamethoxam in cucumber samples ranged from 89% to 97%. Both methods can serve as rapid tools for the detection of thiamethoxam in vegetables.Liya YeXiaoling WuLiguang XuQiankun ZhengHua KuangTaylor & Francis Grouparticlethiamethoxamic-elisamonoclonal antibodycolloidal gold stripAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1173-1183 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	thiamethoxam ic-elisa monoclonal antibody colloidal gold strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	thiamethoxam ic-elisa monoclonal antibody colloidal gold strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Liya Ye Xiaoling Wu Liguang Xu Qiankun Zheng Hua Kuang Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay
description	In this study, we developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for determination of thiamethoxam residues. We prepared a specific highly sensitive monoclonal antibody (mAb) based on a thiamethoxam-derived hapten. After mouse immunisation and cell fusion, we obtained three monoclonal cell lines: 3B2, 3G6, and 4D2. The antibodies produced by cell line 4D2 had the highest affinity and sensitivity. The half-maximal inhibitory concentration was 0.87 ng/mL, and the cross-reactivity was <1%. We optimised the ic-ELISA method and the optimal conditions for ic-ELISA consisted of pH 7.4, 0.8% NaCl, and 0% methanol in PBS buffer. Using this mAb, an ic-ELISA and immunochromatographic strip (ICS) assay were used to detect thiamethoxam residues in cucumbers. The working range of ic-ELISA was 0.5–1.7 ng/mL, and the cut-off vaule of the ICS was 10 ng/mL. The recovery of thiamethoxam in cucumber samples ranged from 89% to 97%. Both methods can serve as rapid tools for the detection of thiamethoxam in vegetables.
format	article
author	Liya Ye Xiaoling Wu Liguang Xu Qiankun Zheng Hua Kuang
author_facet	Liya Ye Xiaoling Wu Liguang Xu Qiankun Zheng Hua Kuang
author_sort	Liya Ye
title	Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay
title_short	Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay
title_full	Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay
title_fullStr	Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay
title_full_unstemmed	Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay
title_sort	preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/fc7e5e908c174bd28ccda83d161527d5
work_keys_str_mv	AT liyaye preparationofanantithiamethoxammonoclonalantibodyfordevelopmentofanindirectcompetitiveenzymelinkedimmunosorbentassayandacolloidalgoldimmunoassay AT xiaolingwu preparationofanantithiamethoxammonoclonalantibodyfordevelopmentofanindirectcompetitiveenzymelinkedimmunosorbentassayandacolloidalgoldimmunoassay AT liguangxu preparationofanantithiamethoxammonoclonalantibodyfordevelopmentofanindirectcompetitiveenzymelinkedimmunosorbentassayandacolloidalgoldimmunoassay AT qiankunzheng preparationofanantithiamethoxammonoclonalantibodyfordevelopmentofanindirectcompetitiveenzymelinkedimmunosorbentassayandacolloidalgoldimmunoassay AT huakuang preparationofanantithiamethoxammonoclonalantibodyfordevelopmentofanindirectcompetitiveenzymelinkedimmunosorbentassayandacolloidalgoldimmunoassay
_version_	1718409573537153024

Preparation of an anti-thiamethoxam monoclonal antibody for development of an indirect competitive enzyme-linked immunosorbent assay and a colloidal gold immunoassay

Ejemplares similares