Anti-Hyperglycemic Effects of Refined Fractions from Cyclocarya paliurus Leaves on Streptozotocin-Induced Diabetic Mice

To identify the chemical components responsible for the anti-hyperglycemic effect of Cyclocarya paliurus (Batal.) Iljinsk (Juglandaceae) leaves, an ethanol extract (CPE) and a water extract (CPW) of C. paliurus leaves, as well as their total flavonoids (CPF), tr...

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Autores principales:	Zheling Feng, Zhujun Fang, Cheng Chen, Chi Teng Vong, Jiali Chen, Ruohan Lou, Maggie Pui Man Hoi, Lishe Gan, Ligen Lin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cyclocarya paliurus</i> flavonoids polysaccharides triterpenoids anti-hyperglycemia Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/fc8504eea2d74c9d84c7bed2f70637b4
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spelling	oai:doaj.org-article:fc8504eea2d74c9d84c7bed2f70637b42021-11-25T18:28:08ZAnti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice10.3390/molecules262268861420-3049https://doaj.org/article/fc8504eea2d74c9d84c7bed2f70637b42021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6886https://doaj.org/toc/1420-3049To identify the chemical components responsible for the anti-hyperglycemic effect of <i>Cyclocarya paliurus</i> (Batal.) Iljinsk (Juglandaceae) leaves, an ethanol extract (CPE) and a water extract (CPW) of <i>C. paliurus</i> leaves, as well as their total flavonoids (CPF), triterpenoids (CPT) and crude polysaccharides (CPP), were prepared and assessed on streptozotocin (STZ)-induced diabetic mice. After being orally administrated once a day for 24 days, CPF (300 mg/kg), CPP (180 mg/kg), or CPF+CPP (300 mg/kg CPF + 180 mg/kg CPP) treatment reversed STZ-induced body weight and muscle mass losses. The glucose tolerance tests and insulin tolerance tests suggested that CPF, CPP, and CPF+CPP showed anti-hyperglycemic effect in STZ-induced diabetic mice. Furthermore, CPF enhances glucose-stimulated insulin secretion in MIN6 cells and insulin-stimulated glucose uptake in C2C12 myotubes. CPF and CPP suppressed inflammatory cytokine levels in STZ-induced diabetic mice. Additionally, CPF and CPP improved STZ-induced diabetic nephropathy assessed by H&E staining, blood urea nitrogen content, and urine creatinine level. The molecular networking and Emperor analysis results indicated that CPF showed potential anti-hyperglycemic effects, and HPLC–MS/MS analysis indicated that CPF contains 3 phenolic acids and 9 flavonoids. In contrast, CPT (650 mg/kg) and CPC (300 mg/kg CPF + 180 mg/kg CPP + 650 mg/kg CPT) did not show anti-hyperglycemic effect. Taken together, polysaccharides and flavonoids are responsible for the anti-hyperglycemic effect of <i>C. paliurus</i> leaves, and the clinical application of <i>C. paliurus</i> need to be refined.Zheling FengZhujun FangCheng ChenChi Teng VongJiali ChenRuohan LouMaggie Pui Man HoiLishe GanLigen LinMDPI AGarticle<i>Cyclocarya paliurus</i>flavonoidspolysaccharidestriterpenoidsanti-hyperglycemiaOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6886, p 6886 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cyclocarya paliurus</i> flavonoids polysaccharides triterpenoids anti-hyperglycemia Organic chemistry QD241-441
spellingShingle	<i>Cyclocarya paliurus</i> flavonoids polysaccharides triterpenoids anti-hyperglycemia Organic chemistry QD241-441 Zheling Feng Zhujun Fang Cheng Chen Chi Teng Vong Jiali Chen Ruohan Lou Maggie Pui Man Hoi Lishe Gan Ligen Lin Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice
description	To identify the chemical components responsible for the anti-hyperglycemic effect of <i>Cyclocarya paliurus</i> (Batal.) Iljinsk (Juglandaceae) leaves, an ethanol extract (CPE) and a water extract (CPW) of <i>C. paliurus</i> leaves, as well as their total flavonoids (CPF), triterpenoids (CPT) and crude polysaccharides (CPP), were prepared and assessed on streptozotocin (STZ)-induced diabetic mice. After being orally administrated once a day for 24 days, CPF (300 mg/kg), CPP (180 mg/kg), or CPF+CPP (300 mg/kg CPF + 180 mg/kg CPP) treatment reversed STZ-induced body weight and muscle mass losses. The glucose tolerance tests and insulin tolerance tests suggested that CPF, CPP, and CPF+CPP showed anti-hyperglycemic effect in STZ-induced diabetic mice. Furthermore, CPF enhances glucose-stimulated insulin secretion in MIN6 cells and insulin-stimulated glucose uptake in C2C12 myotubes. CPF and CPP suppressed inflammatory cytokine levels in STZ-induced diabetic mice. Additionally, CPF and CPP improved STZ-induced diabetic nephropathy assessed by H&E staining, blood urea nitrogen content, and urine creatinine level. The molecular networking and Emperor analysis results indicated that CPF showed potential anti-hyperglycemic effects, and HPLC–MS/MS analysis indicated that CPF contains 3 phenolic acids and 9 flavonoids. In contrast, CPT (650 mg/kg) and CPC (300 mg/kg CPF + 180 mg/kg CPP + 650 mg/kg CPT) did not show anti-hyperglycemic effect. Taken together, polysaccharides and flavonoids are responsible for the anti-hyperglycemic effect of <i>C. paliurus</i> leaves, and the clinical application of <i>C. paliurus</i> need to be refined.
format	article
author	Zheling Feng Zhujun Fang Cheng Chen Chi Teng Vong Jiali Chen Ruohan Lou Maggie Pui Man Hoi Lishe Gan Ligen Lin
author_facet	Zheling Feng Zhujun Fang Cheng Chen Chi Teng Vong Jiali Chen Ruohan Lou Maggie Pui Man Hoi Lishe Gan Ligen Lin
author_sort	Zheling Feng
title	Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice
title_short	Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice
title_full	Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice
title_fullStr	Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice
title_full_unstemmed	Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice
title_sort	anti-hyperglycemic effects of refined fractions from <i>cyclocarya paliurus</i> leaves on streptozotocin-induced diabetic mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fc8504eea2d74c9d84c7bed2f70637b4
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Anti-Hyperglycemic Effects of Refined Fractions from <i>Cyclocarya paliurus</i> Leaves on Streptozotocin-Induced Diabetic Mice

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