One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

The manipulation of diatom genomes is essential for industrial applications based on their metabolic abilities. Here the authors present an efficient multiplex DNA-free gene editing method using CRISPR-Cas9 and counter-selectable markers.

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Autores principales:	Manuel Serif, Gwendoline Dubois, Anne-Laure Finoux, Marie-Ange Teste, Denis Jallet, Fayza Daboussi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/fc8706bd038248e3be9ac9620f125fd2
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spelling	oai:doaj.org-article:fc8706bd038248e3be9ac9620f125fd22021-12-02T17:32:00ZOne-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing10.1038/s41467-018-06378-92041-1723https://doaj.org/article/fc8706bd038248e3be9ac9620f125fd22018-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06378-9https://doaj.org/toc/2041-1723The manipulation of diatom genomes is essential for industrial applications based on their metabolic abilities. Here the authors present an efficient multiplex DNA-free gene editing method using CRISPR-Cas9 and counter-selectable markers.Manuel SerifGwendoline DuboisAnne-Laure FinouxMarie-Ange TesteDenis JalletFayza DaboussiNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Manuel Serif Gwendoline Dubois Anne-Laure Finoux Marie-Ange Teste Denis Jallet Fayza Daboussi One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
description	The manipulation of diatom genomes is essential for industrial applications based on their metabolic abilities. Here the authors present an efficient multiplex DNA-free gene editing method using CRISPR-Cas9 and counter-selectable markers.
format	article
author	Manuel Serif Gwendoline Dubois Anne-Laure Finoux Marie-Ange Teste Denis Jallet Fayza Daboussi
author_facet	Manuel Serif Gwendoline Dubois Anne-Laure Finoux Marie-Ange Teste Denis Jallet Fayza Daboussi
author_sort	Manuel Serif
title	One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
title_short	One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
title_full	One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
title_fullStr	One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
title_full_unstemmed	One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing
title_sort	one-step generation of multiple gene knock-outs in the diatom phaeodactylum tricornutum by dna-free genome editing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/fc8706bd038248e3be9ac9620f125fd2
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One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing

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