Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.

<h4>Background</h4>Endothelial cells (ECs) are continuously exposed to hemodynamic forces imparted by blood flow. While it is known that endothelial behavior can be influenced by cytokine activation or fluid shear, the combined effects of these two independent agonists have yet to be ful...

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Autores principales:	Ryan B Huang, Omolola Eniola-Adefeso
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc88ab988fca48c9b826d56508f81d2f
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spelling	oai:doaj.org-article:fc88ab988fca48c9b826d56508f81d2f2021-11-18T07:26:48ZShear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.1932-620310.1371/journal.pone.0031874https://doaj.org/article/fc88ab988fca48c9b826d56508f81d2f2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22384091/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Endothelial cells (ECs) are continuously exposed to hemodynamic forces imparted by blood flow. While it is known that endothelial behavior can be influenced by cytokine activation or fluid shear, the combined effects of these two independent agonists have yet to be fully elucidated.<h4>Methodology</h4>We investigated EC response to long-term inflammatory cues under physiologically relevant shear conditions via E-selectin expression where monolayers of human umbilical vein ECs were simultaneously exposed to laminar fluid shear and interleukin-1ß (shear-cytokine activation) in a parallel plate flow chamber.<h4>Results and conclusion</h4>Naïve ECs exposed to shear-cytokine activation display significantly higher E-selectin expression for up to 24 hr relative to ECs activated in static (static-cytokine). Peak E-selectin expression occurred after 8-12 hr of continuous shear-cytokine activation contrary to the commonly observed 4-6 hr peak expression in ECs exposed to static-cytokine activation. Cells with some history of high shear conditioning exhibited either high or muted E-selectin expression depending on the durations of the shear pre-conditioning and the ensuing shear-cytokine activation. Overall, the presented data suggest that a high laminar shear enhances acute EC response to interleukin-1ß in naïve or shear-conditioned ECs as may be found in the pathological setting of ischemia/reperfusion injury while conferring rapid E-selectin downregulation to protect against chronic inflammation.Ryan B HuangOmolola Eniola-AdefesoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 2, p e31874 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ryan B Huang Omolola Eniola-Adefeso Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.
description	<h4>Background</h4>Endothelial cells (ECs) are continuously exposed to hemodynamic forces imparted by blood flow. While it is known that endothelial behavior can be influenced by cytokine activation or fluid shear, the combined effects of these two independent agonists have yet to be fully elucidated.<h4>Methodology</h4>We investigated EC response to long-term inflammatory cues under physiologically relevant shear conditions via E-selectin expression where monolayers of human umbilical vein ECs were simultaneously exposed to laminar fluid shear and interleukin-1ß (shear-cytokine activation) in a parallel plate flow chamber.<h4>Results and conclusion</h4>Naïve ECs exposed to shear-cytokine activation display significantly higher E-selectin expression for up to 24 hr relative to ECs activated in static (static-cytokine). Peak E-selectin expression occurred after 8-12 hr of continuous shear-cytokine activation contrary to the commonly observed 4-6 hr peak expression in ECs exposed to static-cytokine activation. Cells with some history of high shear conditioning exhibited either high or muted E-selectin expression depending on the durations of the shear pre-conditioning and the ensuing shear-cytokine activation. Overall, the presented data suggest that a high laminar shear enhances acute EC response to interleukin-1ß in naïve or shear-conditioned ECs as may be found in the pathological setting of ischemia/reperfusion injury while conferring rapid E-selectin downregulation to protect against chronic inflammation.
format	article
author	Ryan B Huang Omolola Eniola-Adefeso
author_facet	Ryan B Huang Omolola Eniola-Adefeso
author_sort	Ryan B Huang
title	Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.
title_short	Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.
title_full	Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.
title_fullStr	Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.
title_full_unstemmed	Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.
title_sort	shear stress modulation of il-1β-induced e-selectin expression in human endothelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/fc88ab988fca48c9b826d56508f81d2f
work_keys_str_mv	AT ryanbhuang shearstressmodulationofil1binducedeselectinexpressioninhumanendothelialcells AT omololaeniolaadefeso shearstressmodulationofil1binducedeselectinexpressioninhumanendothelialcells
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Shear stress modulation of IL-1β-induced E-selectin expression in human endothelial cells.

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