In vivo analysis of choroid plexus morphogenesis in zebrafish.

<h4>Background</h4>The choroid plexus (ChP), a component of the blood-brain barrier (BBB), produces the cerebrospinal fluid (CSF) and as a result plays a role in (i) protecting and nurturing the brain as well as (ii) in coordinating neuronal migration during neurodevelopment. Until now C...

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Autores principales:	Marta García-Lecea, Igor Kondrychyn, Steven H Fong, Zhang-Rui Ye, Vladimir Korzh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc89526b295040b3967b4e9e3a1375d3
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spelling	oai:doaj.org-article:fc89526b295040b3967b4e9e3a1375d32021-11-25T06:18:46ZIn vivo analysis of choroid plexus morphogenesis in zebrafish.1932-620310.1371/journal.pone.0003090https://doaj.org/article/fc89526b295040b3967b4e9e3a1375d32008-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18769618/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The choroid plexus (ChP), a component of the blood-brain barrier (BBB), produces the cerebrospinal fluid (CSF) and as a result plays a role in (i) protecting and nurturing the brain as well as (ii) in coordinating neuronal migration during neurodevelopment. Until now ChP development was not analyzed in living vertebrates due to technical problems.<h4>Methodology/principal findings</h4>We have analyzed the formation of the fourth ventricle ChP of zebrafish in the GFP-tagged enhancer trap transgenic line SqET33-E20 (Gateways) by a combination of in vivo imaging, histology and mutant analysis. This process includes the formation of the tela choroidea (TC), the recruitment of cells from rhombic lips and, finally, the coalescence of TC resulting in formation of ChP. In Notch-deficient mib mutants the first phase of this process is affected with premature GFP expression, deficient cell recruitment into TC and abnormal patterning of ChP. In Hedgehog-deficient smu mutants the second phase of the ChP morphogenesis lacks cell recruitment and TC cells undergo apoptosis.<h4>Conclusions/significance</h4>This study is the first to demonstrate the formation of ChP in vivo revealing a role of Notch and Hedgehog signalling pathways during different developmental phases of this process.Marta García-LeceaIgor KondrychynSteven H FongZhang-Rui YeVladimir KorzhPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 3, Iss 9, p e3090 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta García-Lecea Igor Kondrychyn Steven H Fong Zhang-Rui Ye Vladimir Korzh In vivo analysis of choroid plexus morphogenesis in zebrafish.
description	<h4>Background</h4>The choroid plexus (ChP), a component of the blood-brain barrier (BBB), produces the cerebrospinal fluid (CSF) and as a result plays a role in (i) protecting and nurturing the brain as well as (ii) in coordinating neuronal migration during neurodevelopment. Until now ChP development was not analyzed in living vertebrates due to technical problems.<h4>Methodology/principal findings</h4>We have analyzed the formation of the fourth ventricle ChP of zebrafish in the GFP-tagged enhancer trap transgenic line SqET33-E20 (Gateways) by a combination of in vivo imaging, histology and mutant analysis. This process includes the formation of the tela choroidea (TC), the recruitment of cells from rhombic lips and, finally, the coalescence of TC resulting in formation of ChP. In Notch-deficient mib mutants the first phase of this process is affected with premature GFP expression, deficient cell recruitment into TC and abnormal patterning of ChP. In Hedgehog-deficient smu mutants the second phase of the ChP morphogenesis lacks cell recruitment and TC cells undergo apoptosis.<h4>Conclusions/significance</h4>This study is the first to demonstrate the formation of ChP in vivo revealing a role of Notch and Hedgehog signalling pathways during different developmental phases of this process.
format	article
author	Marta García-Lecea Igor Kondrychyn Steven H Fong Zhang-Rui Ye Vladimir Korzh
author_facet	Marta García-Lecea Igor Kondrychyn Steven H Fong Zhang-Rui Ye Vladimir Korzh
author_sort	Marta García-Lecea
title	In vivo analysis of choroid plexus morphogenesis in zebrafish.
title_short	In vivo analysis of choroid plexus morphogenesis in zebrafish.
title_full	In vivo analysis of choroid plexus morphogenesis in zebrafish.
title_fullStr	In vivo analysis of choroid plexus morphogenesis in zebrafish.
title_full_unstemmed	In vivo analysis of choroid plexus morphogenesis in zebrafish.
title_sort	in vivo analysis of choroid plexus morphogenesis in zebrafish.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/fc89526b295040b3967b4e9e3a1375d3
work_keys_str_mv	AT martagarcialecea invivoanalysisofchoroidplexusmorphogenesisinzebrafish AT igorkondrychyn invivoanalysisofchoroidplexusmorphogenesisinzebrafish AT stevenhfong invivoanalysisofchoroidplexusmorphogenesisinzebrafish AT zhangruiye invivoanalysisofchoroidplexusmorphogenesisinzebrafish AT vladimirkorzh invivoanalysisofchoroidplexusmorphogenesisinzebrafish
_version_	1718413961998630912

In vivo analysis of choroid plexus morphogenesis in zebrafish.

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