Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand

Abstract Merozoite surface protein 9 (MSP9) constitutes a ligand complex involved in erythrocyte invasion by malarial merozoites and is a promising vaccine target. Plasmodium vivax MSP9 (PvMSP9) is immunogenic upon natural malaria exposure. To address whether sequence diversity in PvMSP9 among field...

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Autores principales:	Sunisa Songsaigath, Takashi Makiuchi, Chaturong Putaporntip, Urassaya Pattanawong, Napaporn Kuamsab, Hiroshi Tachibana, Somchai Jongwutiwes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc92a8aa1f0647adbd2f7e6443cfd506
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spelling	oai:doaj.org-article:fc92a8aa1f0647adbd2f7e6443cfd5062021-12-02T14:06:19ZImmunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand10.1038/s41598-021-82928-42045-2322https://doaj.org/article/fc92a8aa1f0647adbd2f7e6443cfd5062021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82928-4https://doaj.org/toc/2045-2322Abstract Merozoite surface protein 9 (MSP9) constitutes a ligand complex involved in erythrocyte invasion by malarial merozoites and is a promising vaccine target. Plasmodium vivax MSP9 (PvMSP9) is immunogenic upon natural malaria exposure. To address whether sequence diversity in PvMSP9 among field isolates could affect natural antibody responses, the recombinant proteins representing two variants each for the N- and the C-terminal domains of PvMSP-9 were used as antigens to assess antibody reactivity among 246 P. vivax-infected patients’ sera from Tak and Ubon Ratchathani Provinces in Thailand. Results revealed that the seropositivity rates of IgG antibodies to the N-terminal antigens were higher than those to the C-terminal antigens (87.80% vs. 67.48%). Most seropositive sera were reactive to both variants, suggesting the presence of common epitopes. Variant-specific antibodies to the N- and the C-terminal antigens were detected in 15.85% and 16.70% of serum samples, respectively. These seropositivity rates were not significant difference between provinces. The seropositivity rates, levels and avidity of anti-PvMSP9 antibodies exhibited positive trends towards increasing malaria episodes. The IgG isotype responses to the N- and the C-terminal antigens were mainly IgG1 and IgG3. The profile of IgG responses may have implications for development of PvMSP9-based vaccine.Sunisa SongsaigathTakashi MakiuchiChaturong PutaporntipUrassaya PattanawongNapaporn KuamsabHiroshi TachibanaSomchai JongwutiwesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sunisa Songsaigath Takashi Makiuchi Chaturong Putaporntip Urassaya Pattanawong Napaporn Kuamsab Hiroshi Tachibana Somchai Jongwutiwes Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand
description	Abstract Merozoite surface protein 9 (MSP9) constitutes a ligand complex involved in erythrocyte invasion by malarial merozoites and is a promising vaccine target. Plasmodium vivax MSP9 (PvMSP9) is immunogenic upon natural malaria exposure. To address whether sequence diversity in PvMSP9 among field isolates could affect natural antibody responses, the recombinant proteins representing two variants each for the N- and the C-terminal domains of PvMSP-9 were used as antigens to assess antibody reactivity among 246 P. vivax-infected patients’ sera from Tak and Ubon Ratchathani Provinces in Thailand. Results revealed that the seropositivity rates of IgG antibodies to the N-terminal antigens were higher than those to the C-terminal antigens (87.80% vs. 67.48%). Most seropositive sera were reactive to both variants, suggesting the presence of common epitopes. Variant-specific antibodies to the N- and the C-terminal antigens were detected in 15.85% and 16.70% of serum samples, respectively. These seropositivity rates were not significant difference between provinces. The seropositivity rates, levels and avidity of anti-PvMSP9 antibodies exhibited positive trends towards increasing malaria episodes. The IgG isotype responses to the N- and the C-terminal antigens were mainly IgG1 and IgG3. The profile of IgG responses may have implications for development of PvMSP9-based vaccine.
format	article
author	Sunisa Songsaigath Takashi Makiuchi Chaturong Putaporntip Urassaya Pattanawong Napaporn Kuamsab Hiroshi Tachibana Somchai Jongwutiwes
author_facet	Sunisa Songsaigath Takashi Makiuchi Chaturong Putaporntip Urassaya Pattanawong Napaporn Kuamsab Hiroshi Tachibana Somchai Jongwutiwes
author_sort	Sunisa Songsaigath
title	Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand
title_short	Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand
title_full	Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand
title_fullStr	Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand
title_full_unstemmed	Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand
title_sort	immunoglobulin g responses to variant forms of plasmodium vivax merozoite surface protein 9 upon natural infection in thailand
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fc92a8aa1f0647adbd2f7e6443cfd506
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Immunoglobulin G responses to variant forms of Plasmodium vivax merozoite surface protein 9 upon natural infection in Thailand

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