The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.

The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture p...

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Autores principales:	Katharine J Crawfurd, John A Raven, Glen L Wheeler, Emily J Baxter, Ian Joint
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc9892889ed040379b259ccf8cab2d47
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spelling	oai:doaj.org-article:fc9892889ed040379b259ccf8cab2d472021-11-18T07:35:26ZThe response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.1932-620310.1371/journal.pone.0026695https://doaj.org/article/fc9892889ed040379b259ccf8cab2d472011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22053201/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture pH changed rapidly because of CO(2) (aq) assimilation and pH targets of 7.8 and 8.1 could not be sustained. Long-term (∼100 generation) pH-auxostat, continuous cultures could be maintained at target pH when cell density was kept low (<2×10(5) cells mL(-1)). After 3 months continuous culture, the C:N ratio was slightly decreased under high CO(2) conditions and red fluorescence per cell was slightly increased. However, no change was detected in photosynthetic efficiency (F(v)/F(m)) or functional cross section of PS II (σ(PSII)). Elevated pCO(2) has been predicted to be beneficial to diatoms due to reduced cost of carbon concentration mechanisms. There was reduced transcription of one putative δ-carbonic anhydrase (CA-4) after 3 months growth at increased CO(2) but 3 other δ-CAs and the small subunit of RUBISCO showed no change. There was no evidence of adaptation or clade selection of T. pseudonana after ∼100 generations at elevated CO(2). On the basis of this long-term culture, pH change of this magnitude in the future ocean may have little effect on T. pseudonana in the absence of genetic adaption.Katharine J CrawfurdJohn A RavenGlen L WheelerEmily J BaxterIan JointPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26695 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katharine J Crawfurd John A Raven Glen L Wheeler Emily J Baxter Ian Joint The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.
description	The effect of ocean acidification conditions has been investigated in cultures of the diatom Thalassiosira pseudonana CCMP1335. Expected end-of-the-century pCO(2) (aq) concentrations of 760 µatm (equivalent to pH 7.8) were compared with present-day condition (380 µatm CO(2), pH 8.1). Batch culture pH changed rapidly because of CO(2) (aq) assimilation and pH targets of 7.8 and 8.1 could not be sustained. Long-term (∼100 generation) pH-auxostat, continuous cultures could be maintained at target pH when cell density was kept low (<2×10(5) cells mL(-1)). After 3 months continuous culture, the C:N ratio was slightly decreased under high CO(2) conditions and red fluorescence per cell was slightly increased. However, no change was detected in photosynthetic efficiency (F(v)/F(m)) or functional cross section of PS II (σ(PSII)). Elevated pCO(2) has been predicted to be beneficial to diatoms due to reduced cost of carbon concentration mechanisms. There was reduced transcription of one putative δ-carbonic anhydrase (CA-4) after 3 months growth at increased CO(2) but 3 other δ-CAs and the small subunit of RUBISCO showed no change. There was no evidence of adaptation or clade selection of T. pseudonana after ∼100 generations at elevated CO(2). On the basis of this long-term culture, pH change of this magnitude in the future ocean may have little effect on T. pseudonana in the absence of genetic adaption.
format	article
author	Katharine J Crawfurd John A Raven Glen L Wheeler Emily J Baxter Ian Joint
author_facet	Katharine J Crawfurd John A Raven Glen L Wheeler Emily J Baxter Ian Joint
author_sort	Katharine J Crawfurd
title	The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.
title_short	The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.
title_full	The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.
title_fullStr	The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.
title_full_unstemmed	The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.
title_sort	response of thalassiosira pseudonana to long-term exposure to increased co2 and decreased ph.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fc9892889ed040379b259ccf8cab2d47
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The response of Thalassiosira pseudonana to long-term exposure to increased CO2 and decreased pH.

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