Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding

Abstract Uzbekistan is one of two doubly landlocked countries in the world, where all rivers are endorheic basins. Although fish diversity is relatively poor in Uzbekistan, the fish fauna of the region has not yet been fully studied. The aim of this study was to establish a reliable barcoding refere...

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Autores principales:	Bakhtiyor Sheraliev, Zuogang Peng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fc9cde3395dc460bba8eda07bdd4dff6
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spelling	oai:doaj.org-article:fc9cde3395dc460bba8eda07bdd4dff62021-12-02T15:10:54ZMolecular diversity of Uzbekistan’s fishes assessed with DNA barcoding10.1038/s41598-021-96487-12045-2322https://doaj.org/article/fc9cde3395dc460bba8eda07bdd4dff62021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96487-1https://doaj.org/toc/2045-2322Abstract Uzbekistan is one of two doubly landlocked countries in the world, where all rivers are endorheic basins. Although fish diversity is relatively poor in Uzbekistan, the fish fauna of the region has not yet been fully studied. The aim of this study was to establish a reliable barcoding reference database for fish in Uzbekistan. A total of 666 specimens, belonging to 59 species within 39 genera, 17 families, and 9 orders, were subjected to polymerase chain reaction amplification in the barcode region and sequenced. The length of the 666 barcodes was 682 bp. The average K2P distances within species, genera, and families were 0.22%, 6.33%, and 16.46%, respectively. The average interspecific distance was approximately 28.8 times higher than the mean intraspecific distance. The Barcode Index Number (BIN) discordance report showed that 666 specimens represented 55 BINs, of which five were singletons, 45 were taxonomically concordant, and five were taxonomically discordant. The barcode gap analysis demonstrated that 89.3% of the fish species examined could be discriminated by DNA barcoding. These results provide new insights into fish diversity in the inland waters of Uzbekistan and can provide a basis for the development of further studies on fish fauna.Bakhtiyor SheralievZuogang PengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bakhtiyor Sheraliev Zuogang Peng Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding
description	Abstract Uzbekistan is one of two doubly landlocked countries in the world, where all rivers are endorheic basins. Although fish diversity is relatively poor in Uzbekistan, the fish fauna of the region has not yet been fully studied. The aim of this study was to establish a reliable barcoding reference database for fish in Uzbekistan. A total of 666 specimens, belonging to 59 species within 39 genera, 17 families, and 9 orders, were subjected to polymerase chain reaction amplification in the barcode region and sequenced. The length of the 666 barcodes was 682 bp. The average K2P distances within species, genera, and families were 0.22%, 6.33%, and 16.46%, respectively. The average interspecific distance was approximately 28.8 times higher than the mean intraspecific distance. The Barcode Index Number (BIN) discordance report showed that 666 specimens represented 55 BINs, of which five were singletons, 45 were taxonomically concordant, and five were taxonomically discordant. The barcode gap analysis demonstrated that 89.3% of the fish species examined could be discriminated by DNA barcoding. These results provide new insights into fish diversity in the inland waters of Uzbekistan and can provide a basis for the development of further studies on fish fauna.
format	article
author	Bakhtiyor Sheraliev Zuogang Peng
author_facet	Bakhtiyor Sheraliev Zuogang Peng
author_sort	Bakhtiyor Sheraliev
title	Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding
title_short	Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding
title_full	Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding
title_fullStr	Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding
title_full_unstemmed	Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding
title_sort	molecular diversity of uzbekistan’s fishes assessed with dna barcoding
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fc9cde3395dc460bba8eda07bdd4dff6
work_keys_str_mv	AT bakhtiyorsheraliev moleculardiversityofuzbekistansfishesassessedwithdnabarcoding AT zuogangpeng moleculardiversityofuzbekistansfishesassessedwithdnabarcoding
_version_	1718387670149758976

Molecular diversity of Uzbekistan’s fishes assessed with DNA barcoding

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