Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution
Terahertz spectroscopy allows for the analysis of vibrations corresponding to the large-scale structural movements and collective dynamics of hydrogen-bonded water molecules. Previously, differences had been detected in the emission spectra of interferon-gamma (IFNγ) solutions surrounded by extremel...
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2021
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oai:doaj.org-article:fc9fdc0d4b55421aaff85612063475422021-11-25T18:41:16ZAntibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution10.3390/pharmaceutics131118641999-4923https://doaj.org/article/fc9fdc0d4b55421aaff85612063475422021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1864https://doaj.org/toc/1999-4923Terahertz spectroscopy allows for the analysis of vibrations corresponding to the large-scale structural movements and collective dynamics of hydrogen-bonded water molecules. Previously, differences had been detected in the emission spectra of interferon-gamma (IFNγ) solutions surrounded by extremely diluted solutions of either IFNγ or antibodies to IFNγ without direct contact compared to a control. Here we aimed to analyse the structural properties of water in a sample of an aqueous solution of IFNγ via terahertz time-domain spectroscopy (THz-TDS). Tubes with the IFNγ solution were immersed in fluidised lactose saturated with test samples (dilutions of antibodies to IFNγ or control) and incubated at 37 °C for 1, 1.5–2, 2.5–3, or 3.5–4 h. Fluidised lactose was chosen since it is an excipient in the manufacture of drugs based on diluted antibodies to IFNγ. After incubation, spectra were recorded within a wavenumber range of 10 to 110 cm<sup>−1</sup> with a resolution of 4 cm<sup>−1</sup>. Lactose saturated with dilutions of antibodies to IFNγ (incubated for more than 2.5 h) changed the structural properties of an IFNγ aqueous solution without direct contact compared to the control. Terahertz spectra revealed stronger intermolecular hydrogen bonds and an increase in the relaxation time of free and weakly bound water molecules. The methodology developed on the basis of THz-TDS could potentially be applied to quality control of pharmaceuticals based on extremely diluted antibodies.Nikita PenkovMDPI AGarticleterahertznew propertieslactoseinterferon-gammahigh dilutionsstructural propertiesPharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1864, p 1864 (2021) |
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terahertz new properties lactose interferon-gamma high dilutions structural properties Pharmacy and materia medica RS1-441 |
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terahertz new properties lactose interferon-gamma high dilutions structural properties Pharmacy and materia medica RS1-441 Nikita Penkov Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution |
| description |
Terahertz spectroscopy allows for the analysis of vibrations corresponding to the large-scale structural movements and collective dynamics of hydrogen-bonded water molecules. Previously, differences had been detected in the emission spectra of interferon-gamma (IFNγ) solutions surrounded by extremely diluted solutions of either IFNγ or antibodies to IFNγ without direct contact compared to a control. Here we aimed to analyse the structural properties of water in a sample of an aqueous solution of IFNγ via terahertz time-domain spectroscopy (THz-TDS). Tubes with the IFNγ solution were immersed in fluidised lactose saturated with test samples (dilutions of antibodies to IFNγ or control) and incubated at 37 °C for 1, 1.5–2, 2.5–3, or 3.5–4 h. Fluidised lactose was chosen since it is an excipient in the manufacture of drugs based on diluted antibodies to IFNγ. After incubation, spectra were recorded within a wavenumber range of 10 to 110 cm<sup>−1</sup> with a resolution of 4 cm<sup>−1</sup>. Lactose saturated with dilutions of antibodies to IFNγ (incubated for more than 2.5 h) changed the structural properties of an IFNγ aqueous solution without direct contact compared to the control. Terahertz spectra revealed stronger intermolecular hydrogen bonds and an increase in the relaxation time of free and weakly bound water molecules. The methodology developed on the basis of THz-TDS could potentially be applied to quality control of pharmaceuticals based on extremely diluted antibodies. |
| format |
article |
| author |
Nikita Penkov |
| author_facet |
Nikita Penkov |
| author_sort |
Nikita Penkov |
| title |
Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution |
| title_short |
Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution |
| title_full |
Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution |
| title_fullStr |
Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution |
| title_full_unstemmed |
Antibodies Processed Using High Dilution Technology Distantly Change Structural Properties of IFNγ Aqueous Solution |
| title_sort |
antibodies processed using high dilution technology distantly change structural properties of ifnγ aqueous solution |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/fc9fdc0d4b55421aaff8561206347542 |
| work_keys_str_mv |
AT nikitapenkov antibodiesprocessedusinghighdilutiontechnologydistantlychangestructuralpropertiesofifngaqueoussolution |
| _version_ |
1718410811317157888 |