Identification and function of ETH receptor networks in the silkworm Bombyx mori

Abstract Insect ecdysis triggering hormones (ETHs) released from endocrine Inka cells act on specific neurons in the central nervous system (CNS) to activate the ecdysis sequence. These primary target neurons express distinct splicing variants of ETH receptor (ETHR-A or ETHR-B). Here, we characteriz...

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Autores principales:	Ivana Daubnerová, Ladislav Roller, Honoo Satake, Chen Zhang, Young-Joon Kim, Dušan Žitňan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fca2086a40484a8e83cf3dc044ce98e8
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spelling	oai:doaj.org-article:fca2086a40484a8e83cf3dc044ce98e82021-12-02T17:51:22ZIdentification and function of ETH receptor networks in the silkworm Bombyx mori10.1038/s41598-021-91022-82045-2322https://doaj.org/article/fca2086a40484a8e83cf3dc044ce98e82021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91022-8https://doaj.org/toc/2045-2322Abstract Insect ecdysis triggering hormones (ETHs) released from endocrine Inka cells act on specific neurons in the central nervous system (CNS) to activate the ecdysis sequence. These primary target neurons express distinct splicing variants of ETH receptor (ETHR-A or ETHR-B). Here, we characterized both ETHR subtypes in the moth Bombyx mori in vitro and mapped spatial and temporal distribution of their expression within the CNS and peripheral organs. In the CNS, we detected non-overlapping expression patterns of each receptor isoform which showed dramatic changes during metamorphosis. Most ETHR-A and a few ETHR-B neurons produce multiple neuropeptides which are downstream signals for the initiation or termination of various phases during the ecdysis sequence. We also described novel roles of different neuropeptides during these processes. Careful examination of peripheral organs revealed ETHRs expression in specific cells of the frontal ganglion (FG), corpora allata (CA), H-organ and Malpighian tubules prior to each ecdysis. These data indicate that PETH and ETH are multifunctional hormones that act via ETHR-A and ETHR-B to control various functions during the entire development—the ecdysis sequence and associated behaviors by the CNS and FG, JH synthesis by the CA, and possible activity of the H-organ and Malpighian tubules.Ivana DaubnerováLadislav RollerHonoo SatakeChen ZhangYoung-Joon KimDušan ŽitňanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-23 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ivana Daubnerová Ladislav Roller Honoo Satake Chen Zhang Young-Joon Kim Dušan Žitňan Identification and function of ETH receptor networks in the silkworm Bombyx mori
description	Abstract Insect ecdysis triggering hormones (ETHs) released from endocrine Inka cells act on specific neurons in the central nervous system (CNS) to activate the ecdysis sequence. These primary target neurons express distinct splicing variants of ETH receptor (ETHR-A or ETHR-B). Here, we characterized both ETHR subtypes in the moth Bombyx mori in vitro and mapped spatial and temporal distribution of their expression within the CNS and peripheral organs. In the CNS, we detected non-overlapping expression patterns of each receptor isoform which showed dramatic changes during metamorphosis. Most ETHR-A and a few ETHR-B neurons produce multiple neuropeptides which are downstream signals for the initiation or termination of various phases during the ecdysis sequence. We also described novel roles of different neuropeptides during these processes. Careful examination of peripheral organs revealed ETHRs expression in specific cells of the frontal ganglion (FG), corpora allata (CA), H-organ and Malpighian tubules prior to each ecdysis. These data indicate that PETH and ETH are multifunctional hormones that act via ETHR-A and ETHR-B to control various functions during the entire development—the ecdysis sequence and associated behaviors by the CNS and FG, JH synthesis by the CA, and possible activity of the H-organ and Malpighian tubules.
format	article
author	Ivana Daubnerová Ladislav Roller Honoo Satake Chen Zhang Young-Joon Kim Dušan Žitňan
author_facet	Ivana Daubnerová Ladislav Roller Honoo Satake Chen Zhang Young-Joon Kim Dušan Žitňan
author_sort	Ivana Daubnerová
title	Identification and function of ETH receptor networks in the silkworm Bombyx mori
title_short	Identification and function of ETH receptor networks in the silkworm Bombyx mori
title_full	Identification and function of ETH receptor networks in the silkworm Bombyx mori
title_fullStr	Identification and function of ETH receptor networks in the silkworm Bombyx mori
title_full_unstemmed	Identification and function of ETH receptor networks in the silkworm Bombyx mori
title_sort	identification and function of eth receptor networks in the silkworm bombyx mori
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fca2086a40484a8e83cf3dc044ce98e8
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Identification and function of ETH receptor networks in the silkworm Bombyx mori

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