Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice

Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressi...

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Autores principales:	Hui Niu, Jun-Hong Xing, Bo-Shi Zou, Chun-Wei Shi, Hai-Bin Huang, Yan-Long Jiang, Jian-Zhong Wang, Xin Cao, Nan Wang, Yan Zeng, Wen-Tao Yang, Gui-Lian Yang, Chun-Feng Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	H7N9 avian influenza HA1 protein DCpep Lactobacillus plantarum immune response Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fca2780671694d1db8d0d54057b11d6a
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spelling	oai:doaj.org-article:fca2780671694d1db8d0d54057b11d6a2021-12-01T21:50:10ZImmune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice1664-322410.3389/fimmu.2021.800965https://doaj.org/article/fca2780671694d1db8d0d54057b11d6a2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.800965/fullhttps://doaj.org/toc/1664-3224Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressing the influenza virus antigen HA1 with the adjuvant dendritic cell-targeting peptide (DCpep). The recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep were used to immunize mice via oral administration, and the humoral, cellular and mucosal immune responses were evaluated. In addition, the serum levels of specific antibodies and hemagglutination inhibition (HI) levels were also measured. Our results showed that recombinant L. plantarum activated dendritic cells in Peyer’s patches (PPs), increased the numbers of CD4+IFN-γ+ and CD8+IFN-γ+ cells in the spleen and mesenteric lymph nodes (MLNs), and affected the ability of CD4+ and CD8+ cells to proliferate in the spleen and MLNs. Additionally, recombinant L. plantarum increased the number of B220+IgA+ cells in PPs and the level of IgA in the lungs and different intestinal segments. In addition, specific IgG, IgG1 and IgG2a antibodies were induced at high levels in the mice serum, specific IgA antibodies were induced at high levels in the mice feces, and HI potency was significantly increased. Thus, the recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep have potential as vaccine candidates for avian influenza virus.Hui NiuJun-Hong XingBo-Shi ZouChun-Wei ShiHai-Bin HuangYan-Long JiangJian-Zhong WangXin CaoNan WangYan ZengWen-Tao YangGui-Lian YangChun-Feng WangFrontiers Media S.A.articleH7N9 avian influenzaHA1 proteinDCpepLactobacillus plantarumimmune responseImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	H7N9 avian influenza HA1 protein DCpep Lactobacillus plantarum immune response Immunologic diseases. Allergy RC581-607
spellingShingle	H7N9 avian influenza HA1 protein DCpep Lactobacillus plantarum immune response Immunologic diseases. Allergy RC581-607 Hui Niu Jun-Hong Xing Bo-Shi Zou Chun-Wei Shi Hai-Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Wen-Tao Yang Gui-Lian Yang Chun-Feng Wang Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice
description	Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressing the influenza virus antigen HA1 with the adjuvant dendritic cell-targeting peptide (DCpep). The recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep were used to immunize mice via oral administration, and the humoral, cellular and mucosal immune responses were evaluated. In addition, the serum levels of specific antibodies and hemagglutination inhibition (HI) levels were also measured. Our results showed that recombinant L. plantarum activated dendritic cells in Peyer’s patches (PPs), increased the numbers of CD4+IFN-γ+ and CD8+IFN-γ+ cells in the spleen and mesenteric lymph nodes (MLNs), and affected the ability of CD4+ and CD8+ cells to proliferate in the spleen and MLNs. Additionally, recombinant L. plantarum increased the number of B220+IgA+ cells in PPs and the level of IgA in the lungs and different intestinal segments. In addition, specific IgG, IgG1 and IgG2a antibodies were induced at high levels in the mice serum, specific IgA antibodies were induced at high levels in the mice feces, and HI potency was significantly increased. Thus, the recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep have potential as vaccine candidates for avian influenza virus.
format	article
author	Hui Niu Jun-Hong Xing Bo-Shi Zou Chun-Wei Shi Hai-Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Wen-Tao Yang Gui-Lian Yang Chun-Feng Wang
author_facet	Hui Niu Jun-Hong Xing Bo-Shi Zou Chun-Wei Shi Hai-Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Wen-Tao Yang Gui-Lian Yang Chun-Feng Wang
author_sort	Hui Niu
title	Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice
title_short	Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice
title_full	Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice
title_fullStr	Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice
title_full_unstemmed	Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice
title_sort	immune evaluation of recombinant lactobacillus plantarum with surface display of ha1-dcpep in mice
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fca2780671694d1db8d0d54057b11d6a
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Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice

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