Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile.
Bauhinia holophylla leaves, also known as "pata-de-vaca", are traditionally used in Brazil to treat diabetes. Although the hypoglycemic activity of this medicinal plant has already been described, the active compounds responsible for the hypoglycemic activity have not yet been identified....
Guardado en:
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/fca2dbe850184936aeb3f5cc3110a136 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:fca2dbe850184936aeb3f5cc3110a136 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:fca2dbe850184936aeb3f5cc3110a1362021-12-02T20:17:27ZHypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile.1932-620310.1371/journal.pone.0258016https://doaj.org/article/fca2dbe850184936aeb3f5cc3110a1362021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258016https://doaj.org/toc/1932-6203Bauhinia holophylla leaves, also known as "pata-de-vaca", are traditionally used in Brazil to treat diabetes. Although the hypoglycemic activity of this medicinal plant has already been described, the active compounds responsible for the hypoglycemic activity have not yet been identified. To rapidly obtain two fractions in large amounts compatible with further in vivo assay, the hydroalcoholic extract of B. holophylla leaves was fractionated by Vacuum Liquid Chromatography and then purified by medium pressure liquid chromatography combined with an in vivo Glucose Tolerance Test in diabetic mice. This approach resulted in the identification of eleven compounds (1-11), including an original non-cyanogenic cyanoglucoside derivative. The structures of the isolated compounds were elucidated by nuclear magnetic resonance and high-resolution mass spectrometry. One of the major compounds of the leaves, lithospermoside (3), exhibited strong hypoglycemic activity in diabetic mice at the doses of 10 and 20 mg/kg b.w. and prevents body weight loss. The proton nuclear magnetic resonance (1H NMR) quantification revealed that the hydroalcoholic leaves extract contained 1.7% of lithospermoside (3) and 3.1% of flavonoids. The NMR analysis also revealed the presence of a high amount of pinitol (4) (9.5%), a known compound possessing in vivo hypoglycemic activity. The hypoglycemic properties of the hydroalcoholic leaves extract and the traditional water infusion extracts of the leaves of B. holophylla seem thus to be the result of the activity of three unrelated classes of compounds. Such results support to some extent the traditional use of Bauhinia holophylla to treat diabetes.Luiz Leonardo SaldanhaAislan Quintiliano DelgadoLaurence MarcourtNathalia Aparecida de Paula CamafortePriscilla Maria Ponce VaredaSamad Nejad EbrahimiWagner VilegasAnne Lígia DokkedalEmerson Ferreira QueirozJean-Luc WolfenderJosé Roberto BosqueiroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0258016 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Luiz Leonardo Saldanha Aislan Quintiliano Delgado Laurence Marcourt Nathalia Aparecida de Paula Camaforte Priscilla Maria Ponce Vareda Samad Nejad Ebrahimi Wagner Vilegas Anne Lígia Dokkedal Emerson Ferreira Queiroz Jean-Luc Wolfender José Roberto Bosqueiro Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile. |
| description |
Bauhinia holophylla leaves, also known as "pata-de-vaca", are traditionally used in Brazil to treat diabetes. Although the hypoglycemic activity of this medicinal plant has already been described, the active compounds responsible for the hypoglycemic activity have not yet been identified. To rapidly obtain two fractions in large amounts compatible with further in vivo assay, the hydroalcoholic extract of B. holophylla leaves was fractionated by Vacuum Liquid Chromatography and then purified by medium pressure liquid chromatography combined with an in vivo Glucose Tolerance Test in diabetic mice. This approach resulted in the identification of eleven compounds (1-11), including an original non-cyanogenic cyanoglucoside derivative. The structures of the isolated compounds were elucidated by nuclear magnetic resonance and high-resolution mass spectrometry. One of the major compounds of the leaves, lithospermoside (3), exhibited strong hypoglycemic activity in diabetic mice at the doses of 10 and 20 mg/kg b.w. and prevents body weight loss. The proton nuclear magnetic resonance (1H NMR) quantification revealed that the hydroalcoholic leaves extract contained 1.7% of lithospermoside (3) and 3.1% of flavonoids. The NMR analysis also revealed the presence of a high amount of pinitol (4) (9.5%), a known compound possessing in vivo hypoglycemic activity. The hypoglycemic properties of the hydroalcoholic leaves extract and the traditional water infusion extracts of the leaves of B. holophylla seem thus to be the result of the activity of three unrelated classes of compounds. Such results support to some extent the traditional use of Bauhinia holophylla to treat diabetes. |
| format |
article |
| author |
Luiz Leonardo Saldanha Aislan Quintiliano Delgado Laurence Marcourt Nathalia Aparecida de Paula Camaforte Priscilla Maria Ponce Vareda Samad Nejad Ebrahimi Wagner Vilegas Anne Lígia Dokkedal Emerson Ferreira Queiroz Jean-Luc Wolfender José Roberto Bosqueiro |
| author_facet |
Luiz Leonardo Saldanha Aislan Quintiliano Delgado Laurence Marcourt Nathalia Aparecida de Paula Camaforte Priscilla Maria Ponce Vareda Samad Nejad Ebrahimi Wagner Vilegas Anne Lígia Dokkedal Emerson Ferreira Queiroz Jean-Luc Wolfender José Roberto Bosqueiro |
| author_sort |
Luiz Leonardo Saldanha |
| title |
Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile. |
| title_short |
Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile. |
| title_full |
Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile. |
| title_fullStr |
Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile. |
| title_full_unstemmed |
Hypoglycemic active principles from the leaves of Bauhinia holophylla: Comprehensive phytochemical characterization and in vivo activity profile. |
| title_sort |
hypoglycemic active principles from the leaves of bauhinia holophylla: comprehensive phytochemical characterization and in vivo activity profile. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/fca2dbe850184936aeb3f5cc3110a136 |
| work_keys_str_mv |
AT luizleonardosaldanha hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT aislanquintilianodelgado hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT laurencemarcourt hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT nathaliaaparecidadepaulacamaforte hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT priscillamariaponcevareda hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT samadnejadebrahimi hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT wagnervilegas hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT anneligiadokkedal hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT emersonferreiraqueiroz hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT jeanlucwolfender hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile AT joserobertobosqueiro hypoglycemicactiveprinciplesfromtheleavesofbauhiniaholophyllacomprehensivephytochemicalcharacterizationandinvivoactivityprofile |
| _version_ |
1718374383927427072 |