Post-integration silencing of piggyBac transposable elements in Aedes aegypti.

The piggyBac transposon, originating in the genome of the Lepidoptera Trichoplusia ni, has a broad host range, making it useful for the development of a number of transposon-based functional genomic technologies including gene vectors, enhancer-, gene- and protein-traps. While capable of being used...

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Autores principales:	Azhahianambi Palavesam, Caroline Esnault, David A O'Brochta
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fcab500f8dd24674a758520ff60dbdf3
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spelling	oai:doaj.org-article:fcab500f8dd24674a758520ff60dbdf32021-11-18T07:38:31ZPost-integration silencing of piggyBac transposable elements in Aedes aegypti.1932-620310.1371/journal.pone.0068454https://doaj.org/article/fcab500f8dd24674a758520ff60dbdf32013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23861905/?tool=EBIhttps://doaj.org/toc/1932-6203The piggyBac transposon, originating in the genome of the Lepidoptera Trichoplusia ni, has a broad host range, making it useful for the development of a number of transposon-based functional genomic technologies including gene vectors, enhancer-, gene- and protein-traps. While capable of being used as a vector for the creation of transgenic insects and insect cell lines, piggyBac has very limited mobility once integrated into the genome of the yellow fever mosquito, Aedes aegypti. A transgenic Aedes aegypti cell line (AagPB8) was created containing three integrated piggyBac elements and the remobilization potential of the elements was tested. The integrated piggyBac elements in AagPB8 were transpositionally silent in the presence of functional transposase, which was shown to be capable of catalyzing the movement of plasmid-borne piggyBac elements in the same cells. The structural integrity of one of the integrated elements along with the quality of element-flanking DNA, which is known to influence transposition rates, were tested in D. melanogaster. The element was found to be structurally intact, capable of transposition and excision in the soma and germ-line of Drosophila melanogaster, and in a DNA sequence context highly conducive to element movement in Drosophila melanogaster. These data show that transpositional silencing of integrated piggyBac elements in the genome of Aedes aegypti appears to be a function of higher scale genome organization or perhaps epigenetic factors, and not due to structural defects or suboptimal integration sites.Azhahianambi PalavesamCaroline EsnaultDavid A O'BrochtaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68454 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Azhahianambi Palavesam Caroline Esnault David A O'Brochta Post-integration silencing of piggyBac transposable elements in Aedes aegypti.
description	The piggyBac transposon, originating in the genome of the Lepidoptera Trichoplusia ni, has a broad host range, making it useful for the development of a number of transposon-based functional genomic technologies including gene vectors, enhancer-, gene- and protein-traps. While capable of being used as a vector for the creation of transgenic insects and insect cell lines, piggyBac has very limited mobility once integrated into the genome of the yellow fever mosquito, Aedes aegypti. A transgenic Aedes aegypti cell line (AagPB8) was created containing three integrated piggyBac elements and the remobilization potential of the elements was tested. The integrated piggyBac elements in AagPB8 were transpositionally silent in the presence of functional transposase, which was shown to be capable of catalyzing the movement of plasmid-borne piggyBac elements in the same cells. The structural integrity of one of the integrated elements along with the quality of element-flanking DNA, which is known to influence transposition rates, were tested in D. melanogaster. The element was found to be structurally intact, capable of transposition and excision in the soma and germ-line of Drosophila melanogaster, and in a DNA sequence context highly conducive to element movement in Drosophila melanogaster. These data show that transpositional silencing of integrated piggyBac elements in the genome of Aedes aegypti appears to be a function of higher scale genome organization or perhaps epigenetic factors, and not due to structural defects or suboptimal integration sites.
format	article
author	Azhahianambi Palavesam Caroline Esnault David A O'Brochta
author_facet	Azhahianambi Palavesam Caroline Esnault David A O'Brochta
author_sort	Azhahianambi Palavesam
title	Post-integration silencing of piggyBac transposable elements in Aedes aegypti.
title_short	Post-integration silencing of piggyBac transposable elements in Aedes aegypti.
title_full	Post-integration silencing of piggyBac transposable elements in Aedes aegypti.
title_fullStr	Post-integration silencing of piggyBac transposable elements in Aedes aegypti.
title_full_unstemmed	Post-integration silencing of piggyBac transposable elements in Aedes aegypti.
title_sort	post-integration silencing of piggybac transposable elements in aedes aegypti.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/fcab500f8dd24674a758520ff60dbdf3
work_keys_str_mv	AT azhahianambipalavesam postintegrationsilencingofpiggybactransposableelementsinaedesaegypti AT carolineesnault postintegrationsilencingofpiggybactransposableelementsinaedesaegypti AT davidaobrochta postintegrationsilencingofpiggybactransposableelementsinaedesaegypti
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Post-integration silencing of piggyBac transposable elements in Aedes aegypti.

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