Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease

Rheumatic heart disease (RHD) remains a severe public health problem in developing countries. Atrial fibrillation (AF) is a medical complication of RHD. Although the understanding of disease pathogenesis has advanced in recent years, the key questions need to be addressed. Transfer RNA–derived small...

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Autores principales:	Zhao-yu Yang, Peng-fei Li, Zhi-qing Li, Tao Tang, Wei Liu, Yang Wang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	rheumatic heart disease atrial fibrillation transfer RNA derived small RNAs transcriptomics biomarker Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/fcad7ac77cb04415a324486630dd50b0
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spelling	oai:doaj.org-article:fcad7ac77cb04415a324486630dd50b02021-12-01T22:17:54ZAltered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease2297-055X10.3389/fcvm.2021.716716https://doaj.org/article/fcad7ac77cb04415a324486630dd50b02021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.716716/fullhttps://doaj.org/toc/2297-055XRheumatic heart disease (RHD) remains a severe public health problem in developing countries. Atrial fibrillation (AF) is a medical complication of RHD. Although the understanding of disease pathogenesis has advanced in recent years, the key questions need to be addressed. Transfer RNA–derived small RNAs (tsRNAs) are a novel type of short non-coding RNAs with potential regulatory functions in various physiological and pathological processes. The present study used tsRNAs sequencing to investigate the relationship between RHD and atrial fibrillation (AF). Three paired cardiac papillary muscles were taken from six rheumatic RHD patients with AF (3 cases) or without AF (3 cases) from January 2016 to January 2017 in Xiangya Hospital, Central South University. A total of 219 precisely matched tsRNAs were identified, and 77 tsRNAs (fold change > 2.0 and P < 0.05) were differently changed. Three tsRNAs (AS-tDR-001269, AS-tDR-001363, AS-tDR-006049) were randomly selected and confirmed by qRT-PCR. The results of qRT-PCR were consistent with tsRNAs sequencing, suggesting the tsRNAs sequencing was reliable. Subsequently, we predicted the target mRNAs of the three tsRNAs. Moreover, we verified the functions of tsRNAs targeting mRNAs in vitro. Finally, bioinformatics analysis indicated that the target genes were abundant in regulation of transcription, DNA binding, intracellular. Most of the genes were predicted to interplay with cytokine-cytokine receptor by KEGG analysis. Our findings uncover the pathological process of AF in RHD through tsRNAs sequencing. This research provides a new perspective for future research on elucidating the mechanism of AF in RHD and offers potential new candidates for the treatment and diagnosis.Zhao-yu YangZhao-yu YangPeng-fei LiZhi-qing LiTao TangTao TangWei LiuWei LiuYang WangYang WangFrontiers Media S.A.articlerheumatic heart diseaseatrial fibrillationtransfer RNA derived small RNAstranscriptomicsbiomarkerDiseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rheumatic heart disease atrial fibrillation transfer RNA derived small RNAs transcriptomics biomarker Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	rheumatic heart disease atrial fibrillation transfer RNA derived small RNAs transcriptomics biomarker Diseases of the circulatory (Cardiovascular) system RC666-701 Zhao-yu Yang Zhao-yu Yang Peng-fei Li Zhi-qing Li Tao Tang Tao Tang Wei Liu Wei Liu Yang Wang Yang Wang Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease
description	Rheumatic heart disease (RHD) remains a severe public health problem in developing countries. Atrial fibrillation (AF) is a medical complication of RHD. Although the understanding of disease pathogenesis has advanced in recent years, the key questions need to be addressed. Transfer RNA–derived small RNAs (tsRNAs) are a novel type of short non-coding RNAs with potential regulatory functions in various physiological and pathological processes. The present study used tsRNAs sequencing to investigate the relationship between RHD and atrial fibrillation (AF). Three paired cardiac papillary muscles were taken from six rheumatic RHD patients with AF (3 cases) or without AF (3 cases) from January 2016 to January 2017 in Xiangya Hospital, Central South University. A total of 219 precisely matched tsRNAs were identified, and 77 tsRNAs (fold change > 2.0 and P < 0.05) were differently changed. Three tsRNAs (AS-tDR-001269, AS-tDR-001363, AS-tDR-006049) were randomly selected and confirmed by qRT-PCR. The results of qRT-PCR were consistent with tsRNAs sequencing, suggesting the tsRNAs sequencing was reliable. Subsequently, we predicted the target mRNAs of the three tsRNAs. Moreover, we verified the functions of tsRNAs targeting mRNAs in vitro. Finally, bioinformatics analysis indicated that the target genes were abundant in regulation of transcription, DNA binding, intracellular. Most of the genes were predicted to interplay with cytokine-cytokine receptor by KEGG analysis. Our findings uncover the pathological process of AF in RHD through tsRNAs sequencing. This research provides a new perspective for future research on elucidating the mechanism of AF in RHD and offers potential new candidates for the treatment and diagnosis.
format	article
author	Zhao-yu Yang Zhao-yu Yang Peng-fei Li Zhi-qing Li Tao Tang Tao Tang Wei Liu Wei Liu Yang Wang Yang Wang
author_facet	Zhao-yu Yang Zhao-yu Yang Peng-fei Li Zhi-qing Li Tao Tang Tao Tang Wei Liu Wei Liu Yang Wang Yang Wang
author_sort	Zhao-yu Yang
title	Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease
title_short	Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease
title_full	Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease
title_fullStr	Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease
title_full_unstemmed	Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease
title_sort	altered expression of transfer-rna-derived small rnas in human with rheumatic heart disease
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fcad7ac77cb04415a324486630dd50b0
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Altered Expression of Transfer-RNA-Derived Small RNAs in Human With Rheumatic Heart Disease

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