Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments

Members in the family of <i>Desulfobulbaceae</i> may be influential in various anaerobic microbial communities, including those in anoxic aquatic sediments and water columns, and within wastewater treatment facilities and bioelectrochemical systems (BESs) such as microbial fuel cells (MF...

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Autores principales:	Cheng Li, Clare E. Reimers, Yvan Alleau
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Desulfobulbaceae</i> bioelectrochemical reactor electrogen electrogenic sulfur oxidation cable bacteria Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fcb9492417924fbdb326815ac009f1da
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spelling	oai:doaj.org-article:fcb9492417924fbdb326815ac009f1da2021-11-25T18:25:10ZUsing Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments10.3390/microorganisms91123292076-2607https://doaj.org/article/fcb9492417924fbdb326815ac009f1da2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2329https://doaj.org/toc/2076-2607Members in the family of <i>Desulfobulbaceae</i> may be influential in various anaerobic microbial communities, including those in anoxic aquatic sediments and water columns, and within wastewater treatment facilities and bioelectrochemical systems (BESs) such as microbial fuel cells (MFCs). However, the diversity and roles of the <i>Desulfobulbaceae</i> in these communities have received little attention, and large portions of this family remain uncultured. Here we expand on findings from an earlier study (Li, Reimers, and Alleau, 2020) to more fully characterize <i>Desulfobulbaceae</i> that became prevalent in biofilms on oxidative electrodes of bioelectrochemical reactors. After incubations, DNA extraction, microbial community analyses, and microscopic examination, we found that a group of uncultured <i>Desulfobulbaceae</i> were greatly enriched on electrode surfaces. These <i>Desulfobulbaceae</i> appeared to form filaments with morphological features ascribed to cable bacteria, but the majority were taxonomically distinct from recognized cable bacteria genera. Thus, the present study provides new information about a group of <i>Desulfobulbaceae</i> that can exhibit filamentous morphologies and respire on the oxidative electrodes. While the phylogeny of cable bacteria is still being defined and updated, further enriching these members can contribute to the overall understanding of cable bacteria and may also lead to identification of successful isolation strategies.Cheng LiClare E. ReimersYvan AlleauMDPI AGarticle<i>Desulfobulbaceae</i>bioelectrochemical reactorelectrogenelectrogenic sulfur oxidationcable bacteriaBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2329, p 2329 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Desulfobulbaceae</i> bioelectrochemical reactor electrogen electrogenic sulfur oxidation cable bacteria Biology (General) QH301-705.5
spellingShingle	<i>Desulfobulbaceae</i> bioelectrochemical reactor electrogen electrogenic sulfur oxidation cable bacteria Biology (General) QH301-705.5 Cheng Li Clare E. Reimers Yvan Alleau Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments
description	Members in the family of <i>Desulfobulbaceae</i> may be influential in various anaerobic microbial communities, including those in anoxic aquatic sediments and water columns, and within wastewater treatment facilities and bioelectrochemical systems (BESs) such as microbial fuel cells (MFCs). However, the diversity and roles of the <i>Desulfobulbaceae</i> in these communities have received little attention, and large portions of this family remain uncultured. Here we expand on findings from an earlier study (Li, Reimers, and Alleau, 2020) to more fully characterize <i>Desulfobulbaceae</i> that became prevalent in biofilms on oxidative electrodes of bioelectrochemical reactors. After incubations, DNA extraction, microbial community analyses, and microscopic examination, we found that a group of uncultured <i>Desulfobulbaceae</i> were greatly enriched on electrode surfaces. These <i>Desulfobulbaceae</i> appeared to form filaments with morphological features ascribed to cable bacteria, but the majority were taxonomically distinct from recognized cable bacteria genera. Thus, the present study provides new information about a group of <i>Desulfobulbaceae</i> that can exhibit filamentous morphologies and respire on the oxidative electrodes. While the phylogeny of cable bacteria is still being defined and updated, further enriching these members can contribute to the overall understanding of cable bacteria and may also lead to identification of successful isolation strategies.
format	article
author	Cheng Li Clare E. Reimers Yvan Alleau
author_facet	Cheng Li Clare E. Reimers Yvan Alleau
author_sort	Cheng Li
title	Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments
title_short	Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments
title_full	Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments
title_fullStr	Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments
title_full_unstemmed	Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments
title_sort	using oxidative electrodes to enrich novel members in the <i>desulfobulbaceae</i> family from intertidal sediments
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fcb9492417924fbdb326815ac009f1da
work_keys_str_mv	AT chengli usingoxidativeelectrodestoenrichnovelmembersintheidesulfobulbaceaeifamilyfromintertidalsediments AT clareereimers usingoxidativeelectrodestoenrichnovelmembersintheidesulfobulbaceaeifamilyfromintertidalsediments AT yvanalleau usingoxidativeelectrodestoenrichnovelmembersintheidesulfobulbaceaeifamilyfromintertidalsediments
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Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments

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