Identification of a T cell gene expression clock obtained by exploiting a MZ twin design

Abstract Many studies investigated age-related changes in gene expression of different tissues, with scarce agreement due to the high number of affecting factors. Similarly, no consensus has been reached on which genes change expression as a function of age and not because of environment. In this st...

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Autores principales:	Daniel Remondini, Nathan Intrator, Claudia Sala, Michela Pierini, Paolo Garagnani, Isabella Zironi, Claudio Franceschi, Stefano Salvioli, Gastone Castellani
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fcba57a15c094879aa81902681fc1178
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spelling	oai:doaj.org-article:fcba57a15c094879aa81902681fc11782021-12-02T12:32:20ZIdentification of a T cell gene expression clock obtained by exploiting a MZ twin design10.1038/s41598-017-05694-22045-2322https://doaj.org/article/fcba57a15c094879aa81902681fc11782017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05694-2https://doaj.org/toc/2045-2322Abstract Many studies investigated age-related changes in gene expression of different tissues, with scarce agreement due to the high number of affecting factors. Similarly, no consensus has been reached on which genes change expression as a function of age and not because of environment. In this study we analysed gene expression of T lymphocytes from 27 healthy monozygotic twin couples, with ages ranging over whole adult lifespan (22 to 98 years). This unique experimental design allowed us to identify genes involved in normative aging, which expression changes independently from environmental factors. We obtained a transcriptomic signature with 125 genes, from which chronological age can be estimated. This signature has been tested in two datasets of same cell type hybridized over two different platforms, showing a significantly better performance compared to random signatures. Moreover, the same signature was applied on a dataset from a different cell type (human muscle). A lower performance was obtained, indicating the possibility that the signature is T cell-specific. As a whole our results suggest that this approach can be useful to identify age-modulated genes.Daniel RemondiniNathan IntratorClaudia SalaMichela PieriniPaolo GaragnaniIsabella ZironiClaudio FranceschiStefano SalvioliGastone CastellaniNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Remondini Nathan Intrator Claudia Sala Michela Pierini Paolo Garagnani Isabella Zironi Claudio Franceschi Stefano Salvioli Gastone Castellani Identification of a T cell gene expression clock obtained by exploiting a MZ twin design
description	Abstract Many studies investigated age-related changes in gene expression of different tissues, with scarce agreement due to the high number of affecting factors. Similarly, no consensus has been reached on which genes change expression as a function of age and not because of environment. In this study we analysed gene expression of T lymphocytes from 27 healthy monozygotic twin couples, with ages ranging over whole adult lifespan (22 to 98 years). This unique experimental design allowed us to identify genes involved in normative aging, which expression changes independently from environmental factors. We obtained a transcriptomic signature with 125 genes, from which chronological age can be estimated. This signature has been tested in two datasets of same cell type hybridized over two different platforms, showing a significantly better performance compared to random signatures. Moreover, the same signature was applied on a dataset from a different cell type (human muscle). A lower performance was obtained, indicating the possibility that the signature is T cell-specific. As a whole our results suggest that this approach can be useful to identify age-modulated genes.
format	article
author	Daniel Remondini Nathan Intrator Claudia Sala Michela Pierini Paolo Garagnani Isabella Zironi Claudio Franceschi Stefano Salvioli Gastone Castellani
author_facet	Daniel Remondini Nathan Intrator Claudia Sala Michela Pierini Paolo Garagnani Isabella Zironi Claudio Franceschi Stefano Salvioli Gastone Castellani
author_sort	Daniel Remondini
title	Identification of a T cell gene expression clock obtained by exploiting a MZ twin design
title_short	Identification of a T cell gene expression clock obtained by exploiting a MZ twin design
title_full	Identification of a T cell gene expression clock obtained by exploiting a MZ twin design
title_fullStr	Identification of a T cell gene expression clock obtained by exploiting a MZ twin design
title_full_unstemmed	Identification of a T cell gene expression clock obtained by exploiting a MZ twin design
title_sort	identification of a t cell gene expression clock obtained by exploiting a mz twin design
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fcba57a15c094879aa81902681fc1178
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Identification of a T cell gene expression clock obtained by exploiting a MZ twin design

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