Nanodiamonds as multi-purpose labels for microscopy

Abstract Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite f...

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Autores principales:	S. R. Hemelaar, P. de Boer, M. Chipaux, W. Zuidema, T. Hamoh, F. Perona Martinez, A. Nagl, J. P. Hoogenboom, B. N. G. Giepmans, R. Schirhagl
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fccea65d2dba4bac96f91e43f8dce320
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spelling	oai:doaj.org-article:fccea65d2dba4bac96f91e43f8dce3202021-12-02T12:32:00ZNanodiamonds as multi-purpose labels for microscopy10.1038/s41598-017-00797-22045-2322https://doaj.org/article/fccea65d2dba4bac96f91e43f8dce3202017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00797-2https://doaj.org/toc/2045-2322Abstract Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite fluorescence using a focused electron beam (cathodoluminescence; CL) for high-resolution localization; and (iii) the potential use for nanoscale sensing. For all these schemes, the development of versatile molecular labeling using relatively small diamonds is essential. Here, we show the direct targeting of a biological molecule with nanodiamonds as small as 70 nm using a streptavidin conjugation and standard antibody labelling approach. We also show internalization of 40 nm sized nanodiamonds. The fluorescence from the nanodiamonds survives osmium-fixation and plastic embedding making them suited for correlative light and electron microscopy. We show that CL can be observed from epon-embedded nanodiamonds, while surface-exposed nanoparticles also stand out in secondary electron (SE) signal due to the exceptionally high diamond SE yield. Finally, we demonstrate the magnetic read-out using fluorescence from diamonds prior to embedding. Thus, our results firmly establish nanodiamonds containing nitrogen-vacancy centers as unique, versatile probes for combining and correlating different types of microscopy, from fluorescence imaging and magnetometry to ultrastructural investigation using electron microscopy.S. R. HemelaarP. de BoerM. ChipauxW. ZuidemaT. HamohF. Perona MartinezA. NaglJ. P. HoogenboomB. N. G. GiepmansR. SchirhaglNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q S. R. Hemelaar P. de Boer M. Chipaux W. Zuidema T. Hamoh F. Perona Martinez A. Nagl J. P. Hoogenboom B. N. G. Giepmans R. Schirhagl Nanodiamonds as multi-purpose labels for microscopy
description	Abstract Nanodiamonds containing fluorescent nitrogen-vacancy centers are increasingly attracting interest for use as a probe in biological microscopy. This interest stems from (i) strong resistance to photobleaching allowing prolonged fluorescence observation times; (ii) the possibility to excite fluorescence using a focused electron beam (cathodoluminescence; CL) for high-resolution localization; and (iii) the potential use for nanoscale sensing. For all these schemes, the development of versatile molecular labeling using relatively small diamonds is essential. Here, we show the direct targeting of a biological molecule with nanodiamonds as small as 70 nm using a streptavidin conjugation and standard antibody labelling approach. We also show internalization of 40 nm sized nanodiamonds. The fluorescence from the nanodiamonds survives osmium-fixation and plastic embedding making them suited for correlative light and electron microscopy. We show that CL can be observed from epon-embedded nanodiamonds, while surface-exposed nanoparticles also stand out in secondary electron (SE) signal due to the exceptionally high diamond SE yield. Finally, we demonstrate the magnetic read-out using fluorescence from diamonds prior to embedding. Thus, our results firmly establish nanodiamonds containing nitrogen-vacancy centers as unique, versatile probes for combining and correlating different types of microscopy, from fluorescence imaging and magnetometry to ultrastructural investigation using electron microscopy.
format	article
author	S. R. Hemelaar P. de Boer M. Chipaux W. Zuidema T. Hamoh F. Perona Martinez A. Nagl J. P. Hoogenboom B. N. G. Giepmans R. Schirhagl
author_facet	S. R. Hemelaar P. de Boer M. Chipaux W. Zuidema T. Hamoh F. Perona Martinez A. Nagl J. P. Hoogenboom B. N. G. Giepmans R. Schirhagl
author_sort	S. R. Hemelaar
title	Nanodiamonds as multi-purpose labels for microscopy
title_short	Nanodiamonds as multi-purpose labels for microscopy
title_full	Nanodiamonds as multi-purpose labels for microscopy
title_fullStr	Nanodiamonds as multi-purpose labels for microscopy
title_full_unstemmed	Nanodiamonds as multi-purpose labels for microscopy
title_sort	nanodiamonds as multi-purpose labels for microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/fccea65d2dba4bac96f91e43f8dce320
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Nanodiamonds as multi-purpose labels for microscopy

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