Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds
Gibberellic acid is a phytohormone that triggers the germination of seeds in a state of dormancy. Through the quantification of this hormone, the physiological condition of seeds of economic importance can be studded. In this work we validated a High-Performance Liquid Chromatography method to q...
Guardado en:
| Autor principal: | |
|---|---|
| Formato: | article |
| Lenguaje: | EN ES |
| Publicado: |
Pontificia Universidad Javeriana
2020
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/fcd159495d3549ceafd94309f1f9e67b |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Gibberellic acid is a phytohormone that triggers the germination of seeds
in a state of dormancy. Through the quantification of this hormone, the
physiological condition of seeds of economic importance can be studded.
In this work we validated a High-Performance Liquid Chromatography
method to quantify gibberellic acid in germinated maize (Zea mays L.)
seeds. Chromatographic conditions included the use of a C-18 reversed-phase
column, acetonitrile-formic acid (1:9 %) as the mobile phase, flow of 0.5 mL
min−1
, and detection at 195 nm. We evaluated our method for seven analytical
parameters. The method was linear for gibberellic acid concentrations from
1.0 mg·kg−1
to 50.0 mg·kg−1
. The method’s limits were 0.3 mg·kg−1
and
1.0 mg·kg−1
for detection and quantification, respectively. The method was
highly precise; we obtained variable but low relative standard deviations
(2.62 % - 12.66 %) for the studied gibberellic acid concentrations. We assessed
accuracy through recovery percentages, ranging from 52.85 % - 63.68 %, for
three gibberellic acid concentrations. We conclude that our analytical method
can be used to measure gibberellic acid during the early stages of maize
germination. In addition, the method could be used for the analysis of other
types of plant matrices.
|
|---|