Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds
Gibberellic acid is a phytohormone that triggers the germination of seeds in a state of dormancy. Through the quantification of this hormone, the physiological condition of seeds of economic importance can be studded. In this work we validated a High-Performance Liquid Chromatography method to q...
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Pontificia Universidad Javeriana
2020
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oai:doaj.org-article:fcd159495d3549ceafd94309f1f9e67b2021-11-16T19:29:38ZValidating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds10.11144/Javeriana.SC25-1.vahp0122-74832027-1352https://doaj.org/article/fcd159495d3549ceafd94309f1f9e67b2020-05-01T00:00:00Zhttps://revistas.javeriana.edu.co/index.php/scientarium/article/view/23795https://doaj.org/toc/0122-7483https://doaj.org/toc/2027-1352Gibberellic acid is a phytohormone that triggers the germination of seeds in a state of dormancy. Through the quantification of this hormone, the physiological condition of seeds of economic importance can be studded. In this work we validated a High-Performance Liquid Chromatography method to quantify gibberellic acid in germinated maize (Zea mays L.) seeds. Chromatographic conditions included the use of a C-18 reversed-phase column, acetonitrile-formic acid (1:9 %) as the mobile phase, flow of 0.5 mL min−1 , and detection at 195 nm. We evaluated our method for seven analytical parameters. The method was linear for gibberellic acid concentrations from 1.0 mg·kg−1 to 50.0 mg·kg−1 . The method’s limits were 0.3 mg·kg−1 and 1.0 mg·kg−1 for detection and quantification, respectively. The method was highly precise; we obtained variable but low relative standard deviations (2.62 % - 12.66 %) for the studied gibberellic acid concentrations. We assessed accuracy through recovery percentages, ranging from 52.85 % - 63.68 %, for three gibberellic acid concentrations. We conclude that our analytical method can be used to measure gibberellic acid during the early stages of maize germination. In addition, the method could be used for the analysis of other types of plant matrices. Juan D. Rivera, , Javier Torres, Yaned M. Correa-NavarroPontificia Universidad Javerianaarticlehigh-performance liquid chromatography; dynamic sonication-assisted solvent extraction (dsase); phytohormones; gibberellic acid; validation; concentration; quantification.Science (General)Q1-390ENESUniversitas Scientiarum, Vol 25, Iss 1, Pp 95-111 (2020) |
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DOAJ |
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DOAJ |
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EN ES |
| topic |
high-performance liquid chromatography; dynamic sonication-assisted solvent extraction (dsase); phytohormones; gibberellic acid; validation; concentration; quantification. Science (General) Q1-390 |
| spellingShingle |
high-performance liquid chromatography; dynamic sonication-assisted solvent extraction (dsase); phytohormones; gibberellic acid; validation; concentration; quantification. Science (General) Q1-390 Juan D. Rivera, , Javier Torres, Yaned M. Correa-Navarro Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds |
| description |
Gibberellic acid is a phytohormone that triggers the germination of seeds
in a state of dormancy. Through the quantification of this hormone, the
physiological condition of seeds of economic importance can be studded.
In this work we validated a High-Performance Liquid Chromatography
method to quantify gibberellic acid in germinated maize (Zea mays L.)
seeds. Chromatographic conditions included the use of a C-18 reversed-phase
column, acetonitrile-formic acid (1:9 %) as the mobile phase, flow of 0.5 mL
min−1
, and detection at 195 nm. We evaluated our method for seven analytical
parameters. The method was linear for gibberellic acid concentrations from
1.0 mg·kg−1
to 50.0 mg·kg−1
. The method’s limits were 0.3 mg·kg−1
and
1.0 mg·kg−1
for detection and quantification, respectively. The method was
highly precise; we obtained variable but low relative standard deviations
(2.62 % - 12.66 %) for the studied gibberellic acid concentrations. We assessed
accuracy through recovery percentages, ranging from 52.85 % - 63.68 %, for
three gibberellic acid concentrations. We conclude that our analytical method
can be used to measure gibberellic acid during the early stages of maize
germination. In addition, the method could be used for the analysis of other
types of plant matrices.
|
| format |
article |
| author |
Juan D. Rivera, , Javier Torres, Yaned M. Correa-Navarro |
| author_facet |
Juan D. Rivera, , Javier Torres, Yaned M. Correa-Navarro |
| author_sort |
Juan D. Rivera, , Javier Torres, Yaned M. Correa-Navarro |
| title |
Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds |
| title_short |
Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds |
| title_full |
Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds |
| title_fullStr |
Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds |
| title_full_unstemmed |
Validating a High-performance Liquid Chromatography method for the quantification of gibberellic acid in germinating maize seeds |
| title_sort |
validating a high-performance liquid chromatography method for the quantification of gibberellic acid in germinating maize seeds |
| publisher |
Pontificia Universidad Javeriana |
| publishDate |
2020 |
| url |
https://doaj.org/article/fcd159495d3549ceafd94309f1f9e67b |
| work_keys_str_mv |
AT juandriverajaviertorresyanedmcorreanavarro validatingahighperformanceliquidchromatographymethodforthequantificationofgibberellicacidingerminatingmaizeseeds |
| _version_ |
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