Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.

Adenosine, prostaglandin E(2), or increased intracellular cyclic AMP concentration each elicit potent anti-inflammatory events in human neutrophils by inhibiting functions such as phagocytosis, superoxide production, adhesion and cytokine release. However, the endogenous molecular pathways mediating...

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Autores principales:	Mireille St-Onge, Aline Dumas, Annick Michaud, Cynthia Laflamme, Andrée-Anne Dussault, Marc Pouliot
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2009
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fcd171f2f956420ea0f36326eec2e57a
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spelling	oai:doaj.org-article:fcd171f2f956420ea0f36326eec2e57a2021-11-25T06:16:36ZImpact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.1932-620310.1371/journal.pone.0004902https://doaj.org/article/fcd171f2f956420ea0f36326eec2e57a2009-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/19295914/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Adenosine, prostaglandin E(2), or increased intracellular cyclic AMP concentration each elicit potent anti-inflammatory events in human neutrophils by inhibiting functions such as phagocytosis, superoxide production, adhesion and cytokine release. However, the endogenous molecular pathways mediating these actions are poorly understood. In the present study, we examined their impact on the gene expression profile of stimulated neutrophils. Purified blood neutrophils from healthy donors were stimulated with a cocktail of inflammatory agonists in the presence of at least one of the following anti-inflammatory agents: adenosine A(2A) receptor agonist CGS 21680, prostaglandin E(2), cyclic-AMP-elevating compounds forskolin and RO 20-1724. Total RNA was analyzed using gene chips and real-time PCR. Genes encoding transcription factors, enzymes and regulatory proteins, as well as secreted cytokines/chemokines showed differential expression. We identified 15 genes for which the anti-inflammatory agents altered mRNA levels. The agents affected the expression profile in remarkably similar fashion, suggesting a central mechanism limiting cell activation. We have identified a set of genes that may be part of important resolution pathways that interfere with cell activation. Identification of these pathways will improve understanding of the capacity of tissues to terminate inflammatory responses and contribute to the development of therapeutic strategies based on endogenous resolution.Mireille St-OngeAline DumasAnnick MichaudCynthia LaflammeAndrée-Anne DussaultMarc PouliotPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 4, Iss 3, p e4902 (2009)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mireille St-Onge Aline Dumas Annick Michaud Cynthia Laflamme Andrée-Anne Dussault Marc Pouliot Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.
description	Adenosine, prostaglandin E(2), or increased intracellular cyclic AMP concentration each elicit potent anti-inflammatory events in human neutrophils by inhibiting functions such as phagocytosis, superoxide production, adhesion and cytokine release. However, the endogenous molecular pathways mediating these actions are poorly understood. In the present study, we examined their impact on the gene expression profile of stimulated neutrophils. Purified blood neutrophils from healthy donors were stimulated with a cocktail of inflammatory agonists in the presence of at least one of the following anti-inflammatory agents: adenosine A(2A) receptor agonist CGS 21680, prostaglandin E(2), cyclic-AMP-elevating compounds forskolin and RO 20-1724. Total RNA was analyzed using gene chips and real-time PCR. Genes encoding transcription factors, enzymes and regulatory proteins, as well as secreted cytokines/chemokines showed differential expression. We identified 15 genes for which the anti-inflammatory agents altered mRNA levels. The agents affected the expression profile in remarkably similar fashion, suggesting a central mechanism limiting cell activation. We have identified a set of genes that may be part of important resolution pathways that interfere with cell activation. Identification of these pathways will improve understanding of the capacity of tissues to terminate inflammatory responses and contribute to the development of therapeutic strategies based on endogenous resolution.
format	article
author	Mireille St-Onge Aline Dumas Annick Michaud Cynthia Laflamme Andrée-Anne Dussault Marc Pouliot
author_facet	Mireille St-Onge Aline Dumas Annick Michaud Cynthia Laflamme Andrée-Anne Dussault Marc Pouliot
author_sort	Mireille St-Onge
title	Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.
title_short	Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.
title_full	Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.
title_fullStr	Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.
title_full_unstemmed	Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.
title_sort	impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.
publisher	Public Library of Science (PLoS)
publishDate	2009
url	https://doaj.org/article/fcd171f2f956420ea0f36326eec2e57a
work_keys_str_mv	AT mireillestonge impactofantiinflammatoryagentsonthegeneexpressionprofileofstimulatedhumanneutrophilsunravelingendogenousresolutionpathways AT alinedumas impactofantiinflammatoryagentsonthegeneexpressionprofileofstimulatedhumanneutrophilsunravelingendogenousresolutionpathways AT annickmichaud impactofantiinflammatoryagentsonthegeneexpressionprofileofstimulatedhumanneutrophilsunravelingendogenousresolutionpathways AT cynthialaflamme impactofantiinflammatoryagentsonthegeneexpressionprofileofstimulatedhumanneutrophilsunravelingendogenousresolutionpathways AT andreeannedussault impactofantiinflammatoryagentsonthegeneexpressionprofileofstimulatedhumanneutrophilsunravelingendogenousresolutionpathways AT marcpouliot impactofantiinflammatoryagentsonthegeneexpressionprofileofstimulatedhumanneutrophilsunravelingendogenousresolutionpathways
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Impact of anti-inflammatory agents on the gene expression profile of stimulated human neutrophils: unraveling endogenous resolution pathways.

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