Impacts of poultry house environment on poultry litter bacterial community composition.

Viral and bacterial pathogens are a significant economic concern to the US broiler industry and the ecological epicenter for poultry pathogens is the mixture of bedding material, chicken excrement and feathers that comprises the litter of a poultry house. This study used high-throughput sequencing t...

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Autores principales:	Michael D Dumas, Shawn W Polson, Don Ritter, Jacques Ravel, Jack Gelb, Robin Morgan, K Eric Wommack
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fcdab53826544af8823fd659a27b5515
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spelling	oai:doaj.org-article:fcdab53826544af8823fd659a27b55152021-11-04T06:08:23ZImpacts of poultry house environment on poultry litter bacterial community composition.1932-620310.1371/journal.pone.0024785https://doaj.org/article/fcdab53826544af8823fd659a27b55152011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21949751/?tool=EBIhttps://doaj.org/toc/1932-6203Viral and bacterial pathogens are a significant economic concern to the US broiler industry and the ecological epicenter for poultry pathogens is the mixture of bedding material, chicken excrement and feathers that comprises the litter of a poultry house. This study used high-throughput sequencing to assess the richness and diversity of poultry litter bacterial communities, and to look for connections between these communities and the environmental characteristics of a poultry house including its history of gangrenous dermatitis (GD). Cluster analysis of 16S rRNA gene sequences revealed differences in the distribution of bacterial phylotypes between Wet and Dry litter samples and between houses. Wet litter contained greater diversity with 90% of total bacterial abundance occurring within the top 214 OTU clusters. In contrast, only 50 clusters accounted for 90% of Dry litter bacterial abundance. The sixth largest OTU cluster across all samples classified as an Arcobacter sp., an emerging human pathogen, occurring in only the Wet litter samples of a house with a modern evaporative cooling system. Ironically, the primary pathogenic clostridial and staphylococcal species associated with GD were not found in any house; however, there were thirteen 16S rRNA gene phylotypes of mostly gram-positive phyla that were unique to GD-affected houses and primarily occurred in Wet litter samples. Overall, the poultry house environment appeared to substantially impact the composition of litter bacterial communities and may play a key role in the emergence of food-borne pathogens.Michael D DumasShawn W PolsonDon RitterJacques RavelJack GelbRobin MorganK Eric WommackPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24785 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael D Dumas Shawn W Polson Don Ritter Jacques Ravel Jack Gelb Robin Morgan K Eric Wommack Impacts of poultry house environment on poultry litter bacterial community composition.
description	Viral and bacterial pathogens are a significant economic concern to the US broiler industry and the ecological epicenter for poultry pathogens is the mixture of bedding material, chicken excrement and feathers that comprises the litter of a poultry house. This study used high-throughput sequencing to assess the richness and diversity of poultry litter bacterial communities, and to look for connections between these communities and the environmental characteristics of a poultry house including its history of gangrenous dermatitis (GD). Cluster analysis of 16S rRNA gene sequences revealed differences in the distribution of bacterial phylotypes between Wet and Dry litter samples and between houses. Wet litter contained greater diversity with 90% of total bacterial abundance occurring within the top 214 OTU clusters. In contrast, only 50 clusters accounted for 90% of Dry litter bacterial abundance. The sixth largest OTU cluster across all samples classified as an Arcobacter sp., an emerging human pathogen, occurring in only the Wet litter samples of a house with a modern evaporative cooling system. Ironically, the primary pathogenic clostridial and staphylococcal species associated with GD were not found in any house; however, there were thirteen 16S rRNA gene phylotypes of mostly gram-positive phyla that were unique to GD-affected houses and primarily occurred in Wet litter samples. Overall, the poultry house environment appeared to substantially impact the composition of litter bacterial communities and may play a key role in the emergence of food-borne pathogens.
format	article
author	Michael D Dumas Shawn W Polson Don Ritter Jacques Ravel Jack Gelb Robin Morgan K Eric Wommack
author_facet	Michael D Dumas Shawn W Polson Don Ritter Jacques Ravel Jack Gelb Robin Morgan K Eric Wommack
author_sort	Michael D Dumas
title	Impacts of poultry house environment on poultry litter bacterial community composition.
title_short	Impacts of poultry house environment on poultry litter bacterial community composition.
title_full	Impacts of poultry house environment on poultry litter bacterial community composition.
title_fullStr	Impacts of poultry house environment on poultry litter bacterial community composition.
title_full_unstemmed	Impacts of poultry house environment on poultry litter bacterial community composition.
title_sort	impacts of poultry house environment on poultry litter bacterial community composition.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/fcdab53826544af8823fd659a27b5515
work_keys_str_mv	AT michaelddumas impactsofpoultryhouseenvironmentonpoultrylitterbacterialcommunitycomposition AT shawnwpolson impactsofpoultryhouseenvironmentonpoultrylitterbacterialcommunitycomposition AT donritter impactsofpoultryhouseenvironmentonpoultrylitterbacterialcommunitycomposition AT jacquesravel impactsofpoultryhouseenvironmentonpoultrylitterbacterialcommunitycomposition AT jackgelb impactsofpoultryhouseenvironmentonpoultrylitterbacterialcommunitycomposition AT robinmorgan impactsofpoultryhouseenvironmentonpoultrylitterbacterialcommunitycomposition AT kericwommack impactsofpoultryhouseenvironmentonpoultrylitterbacterialcommunitycomposition
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Impacts of poultry house environment on poultry litter bacterial community composition.

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