Facile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties

This research work attempts to synthesize iron nanoparticles with Centaurea alba extract. The reported synthesis method serves to be more effective over conventional physical and chemical methods, which is found to be cost effective, recyclable, biocompatible and prevents oxidation of iron oxide nan...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Jian Xiu, Ying Zhang, Bilal Ahamad Paray, Aneela Gulnaz, Mehraj Ud Din War
Formato: article
Lenguaje:EN
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://doaj.org/article/fcdc14fdafcd4ff48979bd6c699084ee
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:fcdc14fdafcd4ff48979bd6c699084ee
record_format dspace
spelling oai:doaj.org-article:fcdc14fdafcd4ff48979bd6c699084ee2021-11-22T04:20:00ZFacile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties1878-535210.1016/j.arabjc.2021.103493https://doaj.org/article/fcdc14fdafcd4ff48979bd6c699084ee2022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1878535221005086https://doaj.org/toc/1878-5352This research work attempts to synthesize iron nanoparticles with Centaurea alba extract. The reported synthesis method serves to be more effective over conventional physical and chemical methods, which is found to be cost effective, recyclable, biocompatible and prevents oxidation of iron oxide nanoparticle as well.As the extract of Centaurea alba possess high content of flavonoids, tannins and phenolic acids, it thereby prevents the oxidation and accumulation iron oxide NPs. The morphological features of the obtained nanoparticle were determined by TEM and SEM imaging techniques. Furthermore, various spectroscopic techniques including UV-Vis, FT-IR has been evaluated.As a part of cellular and molecular studies, the prepared FeNPs was subjected to MTT assay for 48h on normal (HUVEC) cells to evaluate its cytotoxicity. The IC50 of FeNPs and BHT against DPPH free radicals were 287 and 191 µg/mL respectively. Male Wistar rats were selected as the model organism for the in vivo studies and has been categorized into 6 groups, where normal diet was provided to the control group, cholesterol diet was provided to sham group (HCD: 1.50% cholesterol and 24.00% fat) and HCD was provided to other groups. FeNPs were infused at low (100µg Kg-1), moderate (200 µg/Kg) and maximum (400 µg/Kg) doses via gavages. Additionally, atorvastatin (10 mg Kg-1) was provided to the last group through gavages with HCD. Six months has been fixed as a study period for all the groups. Various parameters including total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) was assessed in the blood samples of the test organism at the end of the period. Furthermore, sections of coronary artery and aortic arteries were subjected to histopathological examinations, which showed increase in vessel wall thickness in HCD group, however FeNPstreated groups showed no significant pathological changes. Decrease in TG, TC and LDL-C was observed upon treatment of HCD animals with FeNPs.Jian XiuYing ZhangBilal Ahamad ParayAneela GulnazMehraj Ud Din WarElsevierarticleAtherosclerosisIron oxide nanoparticlesCentaurea albaHypercholesterolemiaLipid profileChemistryQD1-999ENArabian Journal of Chemistry, Vol 15, Iss 1, Pp 103493- (2022)
institution DOAJ
collection DOAJ
language EN
topic Atherosclerosis
Iron oxide nanoparticles
Centaurea alba
Hypercholesterolemia
Lipid profile
Chemistry
QD1-999
spellingShingle Atherosclerosis
Iron oxide nanoparticles
Centaurea alba
Hypercholesterolemia
Lipid profile
Chemistry
QD1-999
Jian Xiu
Ying Zhang
Bilal Ahamad Paray
Aneela Gulnaz
Mehraj Ud Din War
Facile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties
description This research work attempts to synthesize iron nanoparticles with Centaurea alba extract. The reported synthesis method serves to be more effective over conventional physical and chemical methods, which is found to be cost effective, recyclable, biocompatible and prevents oxidation of iron oxide nanoparticle as well.As the extract of Centaurea alba possess high content of flavonoids, tannins and phenolic acids, it thereby prevents the oxidation and accumulation iron oxide NPs. The morphological features of the obtained nanoparticle were determined by TEM and SEM imaging techniques. Furthermore, various spectroscopic techniques including UV-Vis, FT-IR has been evaluated.As a part of cellular and molecular studies, the prepared FeNPs was subjected to MTT assay for 48h on normal (HUVEC) cells to evaluate its cytotoxicity. The IC50 of FeNPs and BHT against DPPH free radicals were 287 and 191 µg/mL respectively. Male Wistar rats were selected as the model organism for the in vivo studies and has been categorized into 6 groups, where normal diet was provided to the control group, cholesterol diet was provided to sham group (HCD: 1.50% cholesterol and 24.00% fat) and HCD was provided to other groups. FeNPs were infused at low (100µg Kg-1), moderate (200 µg/Kg) and maximum (400 µg/Kg) doses via gavages. Additionally, atorvastatin (10 mg Kg-1) was provided to the last group through gavages with HCD. Six months has been fixed as a study period for all the groups. Various parameters including total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and high-density lipoprotein cholesterol (HDL-C) was assessed in the blood samples of the test organism at the end of the period. Furthermore, sections of coronary artery and aortic arteries were subjected to histopathological examinations, which showed increase in vessel wall thickness in HCD group, however FeNPstreated groups showed no significant pathological changes. Decrease in TG, TC and LDL-C was observed upon treatment of HCD animals with FeNPs.
format article
author Jian Xiu
Ying Zhang
Bilal Ahamad Paray
Aneela Gulnaz
Mehraj Ud Din War
author_facet Jian Xiu
Ying Zhang
Bilal Ahamad Paray
Aneela Gulnaz
Mehraj Ud Din War
author_sort Jian Xiu
title Facile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties
title_short Facile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties
title_full Facile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties
title_fullStr Facile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties
title_full_unstemmed Facile preparation of Fe2O3 nanoparticles mediated by Centaurea alba extract and assessment of the anti-atherosclerotic properties
title_sort facile preparation of fe2o3 nanoparticles mediated by centaurea alba extract and assessment of the anti-atherosclerotic properties
publisher Elsevier
publishDate 2022
url https://doaj.org/article/fcdc14fdafcd4ff48979bd6c699084ee
work_keys_str_mv AT jianxiu facilepreparationoffe2o3nanoparticlesmediatedbycentaureaalbaextractandassessmentoftheantiatheroscleroticproperties
AT yingzhang facilepreparationoffe2o3nanoparticlesmediatedbycentaureaalbaextractandassessmentoftheantiatheroscleroticproperties
AT bilalahamadparay facilepreparationoffe2o3nanoparticlesmediatedbycentaureaalbaextractandassessmentoftheantiatheroscleroticproperties
AT aneelagulnaz facilepreparationoffe2o3nanoparticlesmediatedbycentaureaalbaextractandassessmentoftheantiatheroscleroticproperties
AT mehrajuddinwar facilepreparationoffe2o3nanoparticlesmediatedbycentaureaalbaextractandassessmentoftheantiatheroscleroticproperties
_version_ 1718418259473072128