Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods

Despite improvements in imaging techniques during recent years, for many non-model systems the fixation of tissues followed by embedding and sectioning for histochemical or immunohistochemical staining remains an important technique in vertebrate histology. The present study sets out to explore the...

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Autores principales:	B Grunow, T Kirchhoff, T Lange, T Moritz, S Harzsch
Formato:	article
Lenguaje:	EN
Publicado:	Inter-Research 2015
Materias:	Biology (General) QH301-705.5 Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fce11bc72b93453fbfaf1d0e8342290e
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spelling	oai:doaj.org-article:fce11bc72b93453fbfaf1d0e8342290e2021-11-18T09:21:03ZHistochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods1864-77821864-779010.3354/ab00624https://doaj.org/article/fce11bc72b93453fbfaf1d0e8342290e2015-04-01T00:00:00Zhttps://www.int-res.com/abstracts/ab/v23/n3/p251-263/https://doaj.org/toc/1864-7782https://doaj.org/toc/1864-7790Despite improvements in imaging techniques during recent years, for many non-model systems the fixation of tissues followed by embedding and sectioning for histochemical or immunohistochemical staining remains an important technique in vertebrate histology. The present study sets out to explore the preservation of histological sections of fish tissues using different preparation techniques. The quality of transverse vibratome sections from trunk segments of the lesser-spotted dogfish Scyliorhinus canicula, Atlantic sturgeon Acipenser oxyrinchus and zebrafish Danio rerio were compared using different fixatives (formaldehyde, paraformaldehyde and zinc-formaldehyde) and embedding methods (gelatine, agarose and low-temperature melting agarose). Our data show that the quality of the vibratome sections for histochemical staining is strongly dependent upon fixation and embedding media. Although paraformaldehyde fixation results in a more pronounced shrinkage of the trunk segment than the other fixatives used , the quality of the sections and the histochemical staining was best with this fixative in zebrafish and dogfish. Additionally, the embedding methods have a strong influence on the quality of the sections. In the dogfish and sturgeon samples, the preferred embedding media were agarose and low-temperature melting agarose, since gelatine often caused shrinkage of the tissues. In conclusion, for histochemical examinations, the processing protocols for vibratome sectioning need to be adapted individually to each study organism.B GrunowT KirchhoffT LangeT MoritzS HarzschInter-ResearcharticleBiology (General)QH301-705.5MicrobiologyQR1-502ENAquatic Biology, Vol 23, Iss 3, Pp 251-263 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5 Microbiology QR1-502
spellingShingle	Biology (General) QH301-705.5 Microbiology QR1-502 B Grunow T Kirchhoff T Lange T Moritz S Harzsch Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods
description	Despite improvements in imaging techniques during recent years, for many non-model systems the fixation of tissues followed by embedding and sectioning for histochemical or immunohistochemical staining remains an important technique in vertebrate histology. The present study sets out to explore the preservation of histological sections of fish tissues using different preparation techniques. The quality of transverse vibratome sections from trunk segments of the lesser-spotted dogfish Scyliorhinus canicula, Atlantic sturgeon Acipenser oxyrinchus and zebrafish Danio rerio were compared using different fixatives (formaldehyde, paraformaldehyde and zinc-formaldehyde) and embedding methods (gelatine, agarose and low-temperature melting agarose). Our data show that the quality of the vibratome sections for histochemical staining is strongly dependent upon fixation and embedding media. Although paraformaldehyde fixation results in a more pronounced shrinkage of the trunk segment than the other fixatives used , the quality of the sections and the histochemical staining was best with this fixative in zebrafish and dogfish. Additionally, the embedding methods have a strong influence on the quality of the sections. In the dogfish and sturgeon samples, the preferred embedding media were agarose and low-temperature melting agarose, since gelatine often caused shrinkage of the tissues. In conclusion, for histochemical examinations, the processing protocols for vibratome sectioning need to be adapted individually to each study organism.
format	article
author	B Grunow T Kirchhoff T Lange T Moritz S Harzsch
author_facet	B Grunow T Kirchhoff T Lange T Moritz S Harzsch
author_sort	B Grunow
title	Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods
title_short	Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods
title_full	Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods
title_fullStr	Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods
title_full_unstemmed	Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods
title_sort	histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods
publisher	Inter-Research
publishDate	2015
url	https://doaj.org/article/fce11bc72b93453fbfaf1d0e8342290e
work_keys_str_mv	AT bgrunow histochemistryonvibratomesectionsoffishtissueacomparisonoffixationandembeddingmethods AT tkirchhoff histochemistryonvibratomesectionsoffishtissueacomparisonoffixationandembeddingmethods AT tlange histochemistryonvibratomesectionsoffishtissueacomparisonoffixationandembeddingmethods AT tmoritz histochemistryonvibratomesectionsoffishtissueacomparisonoffixationandembeddingmethods AT sharzsch histochemistryonvibratomesectionsoffishtissueacomparisonoffixationandembeddingmethods
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Histochemistry on vibratome sections of fish tissue: a comparison of fixation and embedding methods

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