Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.

In this study we evaluate the efficacy of five vaccine formulations containing different combinations of proteins (FimH; leukotoxin, LKT; and pyolysin, PLO) and/or inactivated whole cells (Escherichia coli, Fusobacterium necrophorum, and Trueperella pyogenes) in preventing postpartum uterine disease...

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Autores principales:	Vinícius Silva Machado, Marcela Luccas de Souza Bicalho, Enoch Brandão de Souza Meira Junior, Rodolfo Rossi, Bruno Leonardo Ribeiro, Svetlana Lima, Thiago Santos, Arieli Kussler, Carla Foditsch, Erika Korzune Ganda, Georgios Oikonomou, Soon Hon Cheong, Robert Owen Gilbert, Rodrigo Carvalho Bicalho
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fce4213edb834c13a8bf5fb19e29ec5c
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spelling	oai:doaj.org-article:fce4213edb834c13a8bf5fb19e29ec5c2021-11-18T08:27:40ZSubcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.1932-620310.1371/journal.pone.0091734https://doaj.org/article/fce4213edb834c13a8bf5fb19e29ec5c2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24638139/?tool=EBIhttps://doaj.org/toc/1932-6203In this study we evaluate the efficacy of five vaccine formulations containing different combinations of proteins (FimH; leukotoxin, LKT; and pyolysin, PLO) and/or inactivated whole cells (Escherichia coli, Fusobacterium necrophorum, and Trueperella pyogenes) in preventing postpartum uterine diseases. Inactivated whole cells were produced using two genetically distinct strains of each bacterial species (E. coli, F. necrophorum, and T. pyogenes). FimH and PLO subunits were produced using recombinant protein expression, and LKT was recovered from culturing a wild F. necrophorum strain. Three subcutaneous vaccines were formulated: Vaccine 1 was composed of inactivated bacterial whole cells and proteins; Vaccine 2 was composed of proteins only; and Vaccine 3 was composed of inactivated bacterial whole cells only. Two intravaginal vaccines were formulated: Vaccine 4 was composed of inactivated bacterial whole cells and proteins; and Vaccine 5 was composed of PLO and LKT. To evaluate vaccine efficacy, a randomized clinical trial was conducted at a commercial dairy farm; 371 spring heifers were allocated randomly into one of six different treatments groups: control, Vaccine 1, Vaccine 2, Vaccine 3, Vaccine 4 and Vaccine 5. Late pregnant heifers assigned to one of the vaccine groups were each vaccinated twice: at 230 and 260 days of pregnancy. When vaccines were evaluated grouped as subcutaneous and intravaginal, the subcutaneous ones were found to significantly reduce the incidence of puerperal metritis. Additionally, subcutaneous vaccination significantly reduced rectal temperature at 6±1 days in milk. Reproduction was improved for cows that received subcutaneous vaccines. In general, vaccination induced a significant increase in serum IgG titers against all antigens, with subcutaneous vaccination again being more effective. In conclusion, subcutaneous vaccination with inactivated bacterial components and/or protein subunits of E. coli, F. necrophorum and T. pyogenes can prevent puerperal metritis during the first lactation of dairy cows, leading to improved reproduction.Vinícius Silva MachadoMarcela Luccas de Souza BicalhoEnoch Brandão de Souza Meira JuniorRodolfo RossiBruno Leonardo RibeiroSvetlana LimaThiago SantosArieli KusslerCarla FoditschErika Korzune GandaGeorgios OikonomouSoon Hon CheongRobert Owen GilbertRodrigo Carvalho BicalhoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e91734 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vinícius Silva Machado Marcela Luccas de Souza Bicalho Enoch Brandão de Souza Meira Junior Rodolfo Rossi Bruno Leonardo Ribeiro Svetlana Lima Thiago Santos Arieli Kussler Carla Foditsch Erika Korzune Ganda Georgios Oikonomou Soon Hon Cheong Robert Owen Gilbert Rodrigo Carvalho Bicalho Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.
description	In this study we evaluate the efficacy of five vaccine formulations containing different combinations of proteins (FimH; leukotoxin, LKT; and pyolysin, PLO) and/or inactivated whole cells (Escherichia coli, Fusobacterium necrophorum, and Trueperella pyogenes) in preventing postpartum uterine diseases. Inactivated whole cells were produced using two genetically distinct strains of each bacterial species (E. coli, F. necrophorum, and T. pyogenes). FimH and PLO subunits were produced using recombinant protein expression, and LKT was recovered from culturing a wild F. necrophorum strain. Three subcutaneous vaccines were formulated: Vaccine 1 was composed of inactivated bacterial whole cells and proteins; Vaccine 2 was composed of proteins only; and Vaccine 3 was composed of inactivated bacterial whole cells only. Two intravaginal vaccines were formulated: Vaccine 4 was composed of inactivated bacterial whole cells and proteins; and Vaccine 5 was composed of PLO and LKT. To evaluate vaccine efficacy, a randomized clinical trial was conducted at a commercial dairy farm; 371 spring heifers were allocated randomly into one of six different treatments groups: control, Vaccine 1, Vaccine 2, Vaccine 3, Vaccine 4 and Vaccine 5. Late pregnant heifers assigned to one of the vaccine groups were each vaccinated twice: at 230 and 260 days of pregnancy. When vaccines were evaluated grouped as subcutaneous and intravaginal, the subcutaneous ones were found to significantly reduce the incidence of puerperal metritis. Additionally, subcutaneous vaccination significantly reduced rectal temperature at 6±1 days in milk. Reproduction was improved for cows that received subcutaneous vaccines. In general, vaccination induced a significant increase in serum IgG titers against all antigens, with subcutaneous vaccination again being more effective. In conclusion, subcutaneous vaccination with inactivated bacterial components and/or protein subunits of E. coli, F. necrophorum and T. pyogenes can prevent puerperal metritis during the first lactation of dairy cows, leading to improved reproduction.
format	article
author	Vinícius Silva Machado Marcela Luccas de Souza Bicalho Enoch Brandão de Souza Meira Junior Rodolfo Rossi Bruno Leonardo Ribeiro Svetlana Lima Thiago Santos Arieli Kussler Carla Foditsch Erika Korzune Ganda Georgios Oikonomou Soon Hon Cheong Robert Owen Gilbert Rodrigo Carvalho Bicalho
author_facet	Vinícius Silva Machado Marcela Luccas de Souza Bicalho Enoch Brandão de Souza Meira Junior Rodolfo Rossi Bruno Leonardo Ribeiro Svetlana Lima Thiago Santos Arieli Kussler Carla Foditsch Erika Korzune Ganda Georgios Oikonomou Soon Hon Cheong Robert Owen Gilbert Rodrigo Carvalho Bicalho
author_sort	Vinícius Silva Machado
title	Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.
title_short	Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.
title_full	Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.
title_fullStr	Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.
title_full_unstemmed	Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.
title_sort	subcutaneous immunization with inactivated bacterial components and purified protein of escherichia coli, fusobacterium necrophorum and trueperella pyogenes prevents puerperal metritis in holstein dairy cows.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fce4213edb834c13a8bf5fb19e29ec5c
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Subcutaneous immunization with inactivated bacterial components and purified protein of Escherichia coli, Fusobacterium necrophorum and Trueperella pyogenes prevents puerperal metritis in Holstein dairy cows.

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