Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.

Nutritional immunity--the withholding of nutrients by the host--has long been recognised as an important factor that shapes bacterial-host interactions. However, the dynamics of nutrient availability within local host niches during fungal infection are poorly defined. We have combined laser ablation...

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Autores principales:	Joanna Potrykus, David Stead, Donna M Maccallum, Dagmar S Urgast, Andrea Raab, Nico van Rooijen, Jörg Feldmann, Alistair J P Brown
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fce54623c55245d09ac1c59cf731c69a
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spelling	oai:doaj.org-article:fce54623c55245d09ac1c59cf731c69a2021-11-18T06:07:28ZFungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.1553-73661553-737410.1371/journal.ppat.1003676https://doaj.org/article/fce54623c55245d09ac1c59cf731c69a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24146619/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Nutritional immunity--the withholding of nutrients by the host--has long been recognised as an important factor that shapes bacterial-host interactions. However, the dynamics of nutrient availability within local host niches during fungal infection are poorly defined. We have combined laser ablation-inductively coupled plasma mass spectrometry (LA-ICP MS), MALDI imaging and immunohistochemistry with microtranscriptomics to examine iron homeostasis in the host and pathogen in the murine model of systemic candidiasis. Dramatic changes in the renal iron landscape occur during disease progression. The infection perturbs global iron homeostasis in the host leading to iron accumulation in the renal medulla. Paradoxically, this is accompanied by nutritional immunity in the renal cortex as iron exclusion zones emerge locally around fungal lesions. These exclusion zones correlate with immune infiltrates and haem oxygenase 1-expressing host cells. This local nutritional immunity decreases iron availability, leading to a switch in iron acquisition mechanisms within mature fungal lesions, as revealed by laser capture microdissection and qRT-PCR analyses. Therefore, a complex interplay of systemic and local events influences iron homeostasis and pathogen-host dynamics during disease progression.Joanna PotrykusDavid SteadDonna M MaccallumDagmar S UrgastAndrea RaabNico van RooijenJörg FeldmannAlistair J P BrownPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 9, Iss 10, p e1003676 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Joanna Potrykus David Stead Donna M Maccallum Dagmar S Urgast Andrea Raab Nico van Rooijen Jörg Feldmann Alistair J P Brown Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.
description	Nutritional immunity--the withholding of nutrients by the host--has long been recognised as an important factor that shapes bacterial-host interactions. However, the dynamics of nutrient availability within local host niches during fungal infection are poorly defined. We have combined laser ablation-inductively coupled plasma mass spectrometry (LA-ICP MS), MALDI imaging and immunohistochemistry with microtranscriptomics to examine iron homeostasis in the host and pathogen in the murine model of systemic candidiasis. Dramatic changes in the renal iron landscape occur during disease progression. The infection perturbs global iron homeostasis in the host leading to iron accumulation in the renal medulla. Paradoxically, this is accompanied by nutritional immunity in the renal cortex as iron exclusion zones emerge locally around fungal lesions. These exclusion zones correlate with immune infiltrates and haem oxygenase 1-expressing host cells. This local nutritional immunity decreases iron availability, leading to a switch in iron acquisition mechanisms within mature fungal lesions, as revealed by laser capture microdissection and qRT-PCR analyses. Therefore, a complex interplay of systemic and local events influences iron homeostasis and pathogen-host dynamics during disease progression.
format	article
author	Joanna Potrykus David Stead Donna M Maccallum Dagmar S Urgast Andrea Raab Nico van Rooijen Jörg Feldmann Alistair J P Brown
author_facet	Joanna Potrykus David Stead Donna M Maccallum Dagmar S Urgast Andrea Raab Nico van Rooijen Jörg Feldmann Alistair J P Brown
author_sort	Joanna Potrykus
title	Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.
title_short	Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.
title_full	Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.
title_fullStr	Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.
title_full_unstemmed	Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.
title_sort	fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/fce54623c55245d09ac1c59cf731c69a
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Fungal iron availability during deep seated candidiasis is defined by a complex interplay involving systemic and local events.

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