Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles

The lysozyme-modified nanoparticles (LY@ZnO NPs) were synthesized by the reduction–oxidation method, and the morphology and structure of LY@ZnO were analyzed by Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (XRD), scanning electron microsclope (SEM), and particle size anal...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kangrui Yuan, Xiaoliu Liu, Jianxin Shi, Wei Liu, Kun Liu, Hongmei Lu, Dudu Wu, Zhi Chen, Chengyu Lu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	enzyme lysozyme ZnO nanoparticles nanomaterials antibacterial activity Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fcea520e53ce494a9105441e5c788806
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fcea520e53ce494a9105441e5c788806
record_format	dspace
spelling	oai:doaj.org-article:fcea520e53ce494a9105441e5c7888062021-12-01T05:28:23ZAntibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles2296-264610.3389/fchem.2021.762255https://doaj.org/article/fcea520e53ce494a9105441e5c7888062021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fchem.2021.762255/fullhttps://doaj.org/toc/2296-2646The lysozyme-modified nanoparticles (LY@ZnO NPs) were synthesized by the reduction–oxidation method, and the morphology and structure of LY@ZnO were analyzed by Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (XRD), scanning electron microsclope (SEM), and particle size analysis. The antibacterial effects of LY@ZnO against Escherichia coli (E. coli, Gram-negative bacteria) and Staphylococcus aureus (S. aureus, Gram-positive bacteria) were discussed by measuring the zone of inhibition (ZOI) and growth inhibition. The antimicrobial experiments showed that the LY@ZnO NPs possessed better antibacterial activity than ZnO. Besides, the antibacterial mechanism of LY@ZnO was also investigated, which was attributed to the generation of reactive oxygen species (ROS). Furthermore, the toxicities of LY@ZnO in vivo and in vitro were discussed by the cell counting kit-8 method and animal experiments, showing that LY@ZnO possessed excellent biocompatibility. Finally, the therapeutic effect of LY@ZnO on a rat skin infection model caused by methicillin-resistant Staphylococcus aureus (MRSA) was also studied, which exhibited good anti-infective activity. Our findings showed that LY@ZnO possessed remarkable antibacterial ability due to its excellent membrane permeability and small particle size. Besides, LY@ZnO also exhibited certain stability and great safety, which showed tremendous prospects for microbial infection in patients. It would also be helpful for a better understanding of the enzyme-modified nanomaterials against bacteria.Kangrui YuanXiaoliu LiuJianxin ShiWei LiuKun LiuHongmei LuDudu WuZhi ChenChengyu LuFrontiers Media S.A.articleenzymelysozymeZnO nanoparticlesnanomaterialsantibacterial activityChemistryQD1-999ENFrontiers in Chemistry, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	enzyme lysozyme ZnO nanoparticles nanomaterials antibacterial activity Chemistry QD1-999
spellingShingle	enzyme lysozyme ZnO nanoparticles nanomaterials antibacterial activity Chemistry QD1-999 Kangrui Yuan Xiaoliu Liu Jianxin Shi Wei Liu Kun Liu Hongmei Lu Dudu Wu Zhi Chen Chengyu Lu Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles
description	The lysozyme-modified nanoparticles (LY@ZnO NPs) were synthesized by the reduction–oxidation method, and the morphology and structure of LY@ZnO were analyzed by Fourier transform infrared (FTIR) spectroscopy, powder X-ray diffraction (XRD), scanning electron microsclope (SEM), and particle size analysis. The antibacterial effects of LY@ZnO against Escherichia coli (E. coli, Gram-negative bacteria) and Staphylococcus aureus (S. aureus, Gram-positive bacteria) were discussed by measuring the zone of inhibition (ZOI) and growth inhibition. The antimicrobial experiments showed that the LY@ZnO NPs possessed better antibacterial activity than ZnO. Besides, the antibacterial mechanism of LY@ZnO was also investigated, which was attributed to the generation of reactive oxygen species (ROS). Furthermore, the toxicities of LY@ZnO in vivo and in vitro were discussed by the cell counting kit-8 method and animal experiments, showing that LY@ZnO possessed excellent biocompatibility. Finally, the therapeutic effect of LY@ZnO on a rat skin infection model caused by methicillin-resistant Staphylococcus aureus (MRSA) was also studied, which exhibited good anti-infective activity. Our findings showed that LY@ZnO possessed remarkable antibacterial ability due to its excellent membrane permeability and small particle size. Besides, LY@ZnO also exhibited certain stability and great safety, which showed tremendous prospects for microbial infection in patients. It would also be helpful for a better understanding of the enzyme-modified nanomaterials against bacteria.
format	article
author	Kangrui Yuan Xiaoliu Liu Jianxin Shi Wei Liu Kun Liu Hongmei Lu Dudu Wu Zhi Chen Chengyu Lu
author_facet	Kangrui Yuan Xiaoliu Liu Jianxin Shi Wei Liu Kun Liu Hongmei Lu Dudu Wu Zhi Chen Chengyu Lu
author_sort	Kangrui Yuan
title	Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles
title_short	Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles
title_full	Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles
title_fullStr	Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles
title_full_unstemmed	Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles
title_sort	antibacterial properties and mechanism of lysozyme-modified zno nanoparticles
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/fcea520e53ce494a9105441e5c788806
work_keys_str_mv	AT kangruiyuan antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT xiaoliuliu antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT jianxinshi antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT weiliu antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT kunliu antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT hongmeilu antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT duduwu antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT zhichen antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles AT chengyulu antibacterialpropertiesandmechanismoflysozymemodifiedznonanoparticles
_version_	1718405580454887424

Antibacterial Properties and Mechanism of Lysozyme-Modified ZnO Nanoparticles

Ejemplares similares