Bacterial DNAemia is associated with serum zonulin levels in older subjects

Abstract The increased presence of bacteria in blood is a plausible contributing factor in the development and progression of aging-associated diseases. In this context, we performed the quantification and the taxonomic profiling of the bacterial DNA in blood samples collected from forty-three older...

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Autores principales:	Giorgio Gargari, Giacomo Mantegazza, Valentina Taverniti, Cristian Del Bo’, Stefano Bernardi, Cristina Andres-Lacueva, Raul González-Domínguez, Paul A. Kroon, Mark S. Winterbone, Antonio Cherubini, Patrizia Riso, Simone Guglielmetti
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fcec252b411f4ef0883221bae767eb15
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spelling	oai:doaj.org-article:fcec252b411f4ef0883221bae767eb152021-12-02T15:00:59ZBacterial DNAemia is associated with serum zonulin levels in older subjects10.1038/s41598-021-90476-02045-2322https://doaj.org/article/fcec252b411f4ef0883221bae767eb152021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90476-0https://doaj.org/toc/2045-2322Abstract The increased presence of bacteria in blood is a plausible contributing factor in the development and progression of aging-associated diseases. In this context, we performed the quantification and the taxonomic profiling of the bacterial DNA in blood samples collected from forty-three older subjects enrolled in a nursing home. Quantitative PCR targeting the 16S rRNA gene revealed that all samples contained detectable amounts of bacterial DNA with a concentration that varied considerably between subjects. Correlation analyses revealed that the bacterial DNAemia (expressed as concentration of 16S rRNA gene copies in blood) significantly associated with the serum levels of zonulin, a marker of intestinal permeability. This result was confirmed by the analysis of a second set of blood samples collected from the same subjects. 16S rRNA gene profiling revealed that most of the bacterial DNA detected in blood was ascribable to the phylum Proteobacteria with a predominance of the genus Pseudomonas. Several control samples were also analyzed to assess the influence of contaminant bacterial DNA potentially originating from reagents and materials. The data reported here suggest that para-cellular permeability of epithelial (and, potentially, endothelial) cell layers may play an important role in bacterial migration into the bloodstream. Bacterial DNAemia is likely to impact on several aspects of host physiology and could underpin the development and prognosis of various diseases in older subjects.Giorgio GargariGiacomo MantegazzaValentina TavernitiCristian Del Bo’Stefano BernardiCristina Andres-LacuevaRaul González-DomínguezPaul A. KroonMark S. WinterboneAntonio CherubiniPatrizia RisoSimone GuglielmettiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Giorgio Gargari Giacomo Mantegazza Valentina Taverniti Cristian Del Bo’ Stefano Bernardi Cristina Andres-Lacueva Raul González-Domínguez Paul A. Kroon Mark S. Winterbone Antonio Cherubini Patrizia Riso Simone Guglielmetti Bacterial DNAemia is associated with serum zonulin levels in older subjects
description	Abstract The increased presence of bacteria in blood is a plausible contributing factor in the development and progression of aging-associated diseases. In this context, we performed the quantification and the taxonomic profiling of the bacterial DNA in blood samples collected from forty-three older subjects enrolled in a nursing home. Quantitative PCR targeting the 16S rRNA gene revealed that all samples contained detectable amounts of bacterial DNA with a concentration that varied considerably between subjects. Correlation analyses revealed that the bacterial DNAemia (expressed as concentration of 16S rRNA gene copies in blood) significantly associated with the serum levels of zonulin, a marker of intestinal permeability. This result was confirmed by the analysis of a second set of blood samples collected from the same subjects. 16S rRNA gene profiling revealed that most of the bacterial DNA detected in blood was ascribable to the phylum Proteobacteria with a predominance of the genus Pseudomonas. Several control samples were also analyzed to assess the influence of contaminant bacterial DNA potentially originating from reagents and materials. The data reported here suggest that para-cellular permeability of epithelial (and, potentially, endothelial) cell layers may play an important role in bacterial migration into the bloodstream. Bacterial DNAemia is likely to impact on several aspects of host physiology and could underpin the development and prognosis of various diseases in older subjects.
format	article
author	Giorgio Gargari Giacomo Mantegazza Valentina Taverniti Cristian Del Bo’ Stefano Bernardi Cristina Andres-Lacueva Raul González-Domínguez Paul A. Kroon Mark S. Winterbone Antonio Cherubini Patrizia Riso Simone Guglielmetti
author_facet	Giorgio Gargari Giacomo Mantegazza Valentina Taverniti Cristian Del Bo’ Stefano Bernardi Cristina Andres-Lacueva Raul González-Domínguez Paul A. Kroon Mark S. Winterbone Antonio Cherubini Patrizia Riso Simone Guglielmetti
author_sort	Giorgio Gargari
title	Bacterial DNAemia is associated with serum zonulin levels in older subjects
title_short	Bacterial DNAemia is associated with serum zonulin levels in older subjects
title_full	Bacterial DNAemia is associated with serum zonulin levels in older subjects
title_fullStr	Bacterial DNAemia is associated with serum zonulin levels in older subjects
title_full_unstemmed	Bacterial DNAemia is associated with serum zonulin levels in older subjects
title_sort	bacterial dnaemia is associated with serum zonulin levels in older subjects
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fcec252b411f4ef0883221bae767eb15
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Bacterial DNAemia is associated with serum zonulin levels in older subjects

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