Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12

ABSTRACT Horizontal gene transfer is a major driving force behind the genomic diversity seen in prokaryotes. The cryptic rac prophage in Escherichia coli K-12 carries the gene for a putative transcription factor RacR, whose deletion is lethal. We have shown that the essentiality of racR in E. coli K...

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Autores principales:	Revathy Krishnamurthi, Swagatha Ghosh, Supriya Khedkar, Aswin Sai Narain Seshasayee
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	prophages toxin-antitoxin transcriptional repressor Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/fced5eda6091438eaaf243903479c192
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spelling	oai:doaj.org-article:fced5eda6091438eaaf243903479c1922021-11-15T15:21:53ZRepression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-1210.1128/mSphere.00392-172379-5042https://doaj.org/article/fced5eda6091438eaaf243903479c1922017-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00392-17https://doaj.org/toc/2379-5042ABSTRACT Horizontal gene transfer is a major driving force behind the genomic diversity seen in prokaryotes. The cryptic rac prophage in Escherichia coli K-12 carries the gene for a putative transcription factor RacR, whose deletion is lethal. We have shown that the essentiality of racR in E. coli K-12 is attributed to its role in transcriptionally repressing toxin gene(s) called ydaS and ydaT, which are adjacent to and coded divergently to racR. IMPORTANCE Transcription factors in the bacterium E. coli are rarely essential, and when they are essential, they are largely toxin-antitoxin systems. While studying transcription factors encoded in horizontally acquired regions in E. coli, we realized that the protein RacR, a putative transcription factor encoded by a gene on the rac prophage, is an essential protein. Here, using genetics, biochemistry, and bioinformatics, we show that its essentiality derives from its role as a transcriptional repressor of the ydaS and ydaT genes, whose products are toxic to the cell. Unlike type II toxin-antitoxin systems in which transcriptional regulation involves complexes of the toxin and antitoxin, repression by RacR is sufficient to keep ydaS transcriptionally silent.Revathy KrishnamurthiSwagatha GhoshSupriya KhedkarAswin Sai Narain SeshasayeeAmerican Society for Microbiologyarticleprophagestoxin-antitoxintranscriptional repressorMicrobiologyQR1-502ENmSphere, Vol 2, Iss 6 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	prophages toxin-antitoxin transcriptional repressor Microbiology QR1-502
spellingShingle	prophages toxin-antitoxin transcriptional repressor Microbiology QR1-502 Revathy Krishnamurthi Swagatha Ghosh Supriya Khedkar Aswin Sai Narain Seshasayee Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12
description	ABSTRACT Horizontal gene transfer is a major driving force behind the genomic diversity seen in prokaryotes. The cryptic rac prophage in Escherichia coli K-12 carries the gene for a putative transcription factor RacR, whose deletion is lethal. We have shown that the essentiality of racR in E. coli K-12 is attributed to its role in transcriptionally repressing toxin gene(s) called ydaS and ydaT, which are adjacent to and coded divergently to racR. IMPORTANCE Transcription factors in the bacterium E. coli are rarely essential, and when they are essential, they are largely toxin-antitoxin systems. While studying transcription factors encoded in horizontally acquired regions in E. coli, we realized that the protein RacR, a putative transcription factor encoded by a gene on the rac prophage, is an essential protein. Here, using genetics, biochemistry, and bioinformatics, we show that its essentiality derives from its role as a transcriptional repressor of the ydaS and ydaT genes, whose products are toxic to the cell. Unlike type II toxin-antitoxin systems in which transcriptional regulation involves complexes of the toxin and antitoxin, repression by RacR is sufficient to keep ydaS transcriptionally silent.
format	article
author	Revathy Krishnamurthi Swagatha Ghosh Supriya Khedkar Aswin Sai Narain Seshasayee
author_facet	Revathy Krishnamurthi Swagatha Ghosh Supriya Khedkar Aswin Sai Narain Seshasayee
author_sort	Revathy Krishnamurthi
title	Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12
title_short	Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12
title_full	Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12
title_fullStr	Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12
title_full_unstemmed	Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12
title_sort	repression of ydas toxin is mediated by transcriptional repressor racr in the cryptic <italic toggle="yes">rac</italic> prophage of <named-content content-type="genus-species">escherichia coli</named-content> k-12
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/fced5eda6091438eaaf243903479c192
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Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic <italic toggle="yes">rac</italic> Prophage of <named-content content-type="genus-species">Escherichia coli</named-content> K-12

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