Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.

The stellate ganglia are the predominant source of sympathetic innervation to the heart. Remodeling of sympathetic nerves projecting to the heart has been observed in several cardiovascular diseases, and sympathetic dysfunction contributes to cardiac pathology. Wistar Kyoto rats are a common model f...

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Autores principales:	Richard G Bayles, Joanne Tran, Antoinette Olivas, William R Woodward, Suzanne S Fei, Lina Gao, Beth A Habecker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fcee4ee5a1cd49daa26dd63152220045
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spelling	oai:doaj.org-article:fcee4ee5a1cd49daa26dd631522200452021-12-02T20:18:57ZSex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.1932-620310.1371/journal.pone.0218133https://doaj.org/article/fcee4ee5a1cd49daa26dd631522200452019-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0218133https://doaj.org/toc/1932-6203The stellate ganglia are the predominant source of sympathetic innervation to the heart. Remodeling of sympathetic nerves projecting to the heart has been observed in several cardiovascular diseases, and sympathetic dysfunction contributes to cardiac pathology. Wistar Kyoto rats are a common model for the study of cardiovascular diseases, but we lack a profile of the baseline transcriptomic and neurochemical characteristics of their cardiac sympathetic neurons. Most studies of cardiovascular disease have used male animals only, but in the future both male and female animals will be used for these types of studies; therefore, we sought to characterize the transcriptome of male and female stellate ganglia and to correlate that with catecholamine and acetylcholine content in the heart. We have generated a dataset of baseline RNA expression in male and female Wistar Kyoto rat stellate ganglia using RNA-seq, and have measured neurotransmitter levels in heart and stellate ganglia using HPLC and mass spectrometry. We identified numerous gene expression differences between male and female stellates, including genes encoding important developmental factors, receptors and neuropeptides. Female hearts had significantly higher neurotransmitter content than male hearts; however, no significant differences were detected in expression of the genes encoding neurotransmitter synthetic enzymes. Similarly, no statistically significant differences were identified between the sexes in cardiac tyrosine hydroxylase levels.Richard G BaylesJoanne TranAntoinette OlivasWilliam R WoodwardSuzanne S FeiLina GaoBeth A HabeckerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 14, Iss 6, p e0218133 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Richard G Bayles Joanne Tran Antoinette Olivas William R Woodward Suzanne S Fei Lina Gao Beth A Habecker Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.
description	The stellate ganglia are the predominant source of sympathetic innervation to the heart. Remodeling of sympathetic nerves projecting to the heart has been observed in several cardiovascular diseases, and sympathetic dysfunction contributes to cardiac pathology. Wistar Kyoto rats are a common model for the study of cardiovascular diseases, but we lack a profile of the baseline transcriptomic and neurochemical characteristics of their cardiac sympathetic neurons. Most studies of cardiovascular disease have used male animals only, but in the future both male and female animals will be used for these types of studies; therefore, we sought to characterize the transcriptome of male and female stellate ganglia and to correlate that with catecholamine and acetylcholine content in the heart. We have generated a dataset of baseline RNA expression in male and female Wistar Kyoto rat stellate ganglia using RNA-seq, and have measured neurotransmitter levels in heart and stellate ganglia using HPLC and mass spectrometry. We identified numerous gene expression differences between male and female stellates, including genes encoding important developmental factors, receptors and neuropeptides. Female hearts had significantly higher neurotransmitter content than male hearts; however, no significant differences were detected in expression of the genes encoding neurotransmitter synthetic enzymes. Similarly, no statistically significant differences were identified between the sexes in cardiac tyrosine hydroxylase levels.
format	article
author	Richard G Bayles Joanne Tran Antoinette Olivas William R Woodward Suzanne S Fei Lina Gao Beth A Habecker
author_facet	Richard G Bayles Joanne Tran Antoinette Olivas William R Woodward Suzanne S Fei Lina Gao Beth A Habecker
author_sort	Richard G Bayles
title	Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.
title_short	Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.
title_full	Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.
title_fullStr	Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.
title_full_unstemmed	Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.
title_sort	sex differences in sympathetic gene expression and cardiac neurochemistry in wistar kyoto rats.
publisher	Public Library of Science (PLoS)
publishDate	2019
url	https://doaj.org/article/fcee4ee5a1cd49daa26dd63152220045
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Sex differences in sympathetic gene expression and cardiac neurochemistry in Wistar Kyoto rats.

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