Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles

Jelle Penders,1,2 Michelle Stolzoff,3 Daniel J Hickey,1 Martin Andersson,2 Thomas J Webster1 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Göteborg, Sweden; 3Department of...

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Autores principales:	Penders J, Stolzoff M, Hickey DJ, Andersson M, Webster TJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Gold nanoparticles nanoflowers nanostars S. aureus fibroblasts Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fcf4afb3827147d2822fb3e48730a8ae
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spelling	oai:doaj.org-article:fcf4afb3827147d2822fb3e48730a8ae2021-12-02T00:31:21ZShape-dependent antibacterial effects of non-cytotoxic gold nanoparticles1178-2013https://doaj.org/article/fcf4afb3827147d2822fb3e48730a8ae2017-03-01T00:00:00Zhttps://www.dovepress.com/shape-dependent-antibacterial-effects-of-non-cytotoxic-gold-nanopartic-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jelle Penders,1,2 Michelle Stolzoff,3 Daniel J Hickey,1 Martin Andersson,2 Thomas J Webster1 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Göteborg, Sweden; 3Department of Bioengineering, Northeastern University, Boston, MA, USA Abstract: Gold nanoparticles (AuNPs) of various shapes (including spheres, stars and flowers), with similar dimensions, were synthesized and evaluated for their antibacterial effects toward Staphylococcus aureus, a bacterium responsible for numerous life-threatening infections worldwide. Optical growth curve measurements and Gompertz modeling showed significant AuNP shape- and concentration-dependent decreases in bacterial growth with increases in bacterial growth lag time. To evaluate prospective use in in vivo systems, the cytotoxicity of the same AuNPs was evaluated toward human dermal fibroblasts in vitro by 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) viability assays and confocal microscopy. No indication of any mammalian cell toxicity or morphological effects was found. Additionally, it was observed that the AuNPs were readily internalized in fibroblasts after 4 days of incubation. Most importantly, the results of the present study showed that gold nanoflowers in particular possessed the most promising non-cytotoxic mammalian cell behavior with the greatest shape-dependent antibacterial activity-promising properties for their future investigation in a wide range of anti-infection applications. Keywords: gold, nanoparticles, nanoflowers, nanostars, S. aureus, fibroblastsPenders JStolzoff MHickey DJAndersson MWebster TJDove Medical PressarticleGoldnanoparticlesnanoflowersnanostarsS. aureusfibroblastsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 2457-2468 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Gold nanoparticles nanoflowers nanostars S. aureus fibroblasts Medicine (General) R5-920
spellingShingle	Gold nanoparticles nanoflowers nanostars S. aureus fibroblasts Medicine (General) R5-920 Penders J Stolzoff M Hickey DJ Andersson M Webster TJ Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles
description	Jelle Penders,1,2 Michelle Stolzoff,3 Daniel J Hickey,1 Martin Andersson,2 Thomas J Webster1 1Department of Chemical Engineering, Northeastern University, Boston, MA, USA; 2Department of Chemistry and Chemical Engineering, Chalmers University of Technology, Göteborg, Sweden; 3Department of Bioengineering, Northeastern University, Boston, MA, USA Abstract: Gold nanoparticles (AuNPs) of various shapes (including spheres, stars and flowers), with similar dimensions, were synthesized and evaluated for their antibacterial effects toward Staphylococcus aureus, a bacterium responsible for numerous life-threatening infections worldwide. Optical growth curve measurements and Gompertz modeling showed significant AuNP shape- and concentration-dependent decreases in bacterial growth with increases in bacterial growth lag time. To evaluate prospective use in in vivo systems, the cytotoxicity of the same AuNPs was evaluated toward human dermal fibroblasts in vitro by 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) viability assays and confocal microscopy. No indication of any mammalian cell toxicity or morphological effects was found. Additionally, it was observed that the AuNPs were readily internalized in fibroblasts after 4 days of incubation. Most importantly, the results of the present study showed that gold nanoflowers in particular possessed the most promising non-cytotoxic mammalian cell behavior with the greatest shape-dependent antibacterial activity-promising properties for their future investigation in a wide range of anti-infection applications. Keywords: gold, nanoparticles, nanoflowers, nanostars, S. aureus, fibroblasts
format	article
author	Penders J Stolzoff M Hickey DJ Andersson M Webster TJ
author_facet	Penders J Stolzoff M Hickey DJ Andersson M Webster TJ
author_sort	Penders J
title	Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles
title_short	Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles
title_full	Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles
title_fullStr	Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles
title_full_unstemmed	Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles
title_sort	shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/fcf4afb3827147d2822fb3e48730a8ae
work_keys_str_mv	AT pendersj shapedependentantibacterialeffectsofnoncytotoxicgoldnanoparticles AT stolzoffm shapedependentantibacterialeffectsofnoncytotoxicgoldnanoparticles AT hickeydj shapedependentantibacterialeffectsofnoncytotoxicgoldnanoparticles AT anderssonm shapedependentantibacterialeffectsofnoncytotoxicgoldnanoparticles AT webstertj shapedependentantibacterialeffectsofnoncytotoxicgoldnanoparticles
_version_	1718403716750508032

Shape-dependent antibacterial effects of non-cytotoxic gold nanoparticles

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