Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study

Abstract Background Mitochondria dysfunction is one of the clinical features of diabetes mellitus (DM), which is a hallmark of insulin resistance (IR). This study investigates the therapeutic effect of Momordica charantia nanoparticles on mitochondria biogenesis in diabetic-induced rats. Forty-two a...

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Autores principales:	Olusola Olalekan Elekofehinti, Olusola Christianah Ayodele, Opeyemi Iwaloye
Formato:	article
Lenguaje:	EN
Publicado:	SpringerOpen 2021
Materias:	Diabetes mellitus Mitochondria biogenesis Inflammation Glucose sensitivity Momordica charantia nanoparticles Medicine (General) R5-920 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/fcf74983362a4fa8bbc674abd6799b99
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spelling	oai:doaj.org-article:fcf74983362a4fa8bbc674abd6799b992021-11-14T12:07:22ZMomordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study10.1186/s43042-021-00200-w2090-2441https://doaj.org/article/fcf74983362a4fa8bbc674abd6799b992021-11-01T00:00:00Zhttps://doi.org/10.1186/s43042-021-00200-whttps://doaj.org/toc/2090-2441Abstract Background Mitochondria dysfunction is one of the clinical features of diabetes mellitus (DM), which is a hallmark of insulin resistance (IR). This study investigates the therapeutic effect of Momordica charantia nanoparticles on mitochondria biogenesis in diabetic-induced rats. Forty-two adult wistar rats (average weight of 189 ± 10.32) were grouped as follows: STZ (65 mg/kg), control group, STZ + silver nitrate (10 mg/kg), STZ + M. charantia silver nanoparticles (50 mg/kg), STZ + metformin (100 mg/kg), and STZ + M. charantia aqueous extract (100 mg/kg). DM was induced intraperitoneal using freshly prepared solution of STZ (65 mg/kg), and rats with fasting blood sugar (FBS) above 250 mg/dl after 72 h of induction were considered diabetic. Treatment started after the third day of induction and lasted for 11 days. Effect of M. charantia nanoparticles on glucose level and pancreatic expression of genes involved in mitochondria biogenesis (PGC-1α, AMPK, GSK-3β, PPARϒ), inflammation (IL-1B, TNFα) and glucose sensitivity (PI3K, AKT, PTEN Insulin and Glut2) were quantified using reverse-transcriptase polymerase chain reaction (RT-PCR). Results The results showed that M. charantia nanoparticles promote mitochondria biogenesis, glucose sensitivity and reverse inflammation in the pancreas of diabetes rat model through upregulation of PGC-1α, AMPK, PPARϒ, AKT, Insulin and Glut2 mRNA expression and downregulation of GSK-3β, PI3K, IL-1B and TNFα mRNA expression in the pancreas of diabetic rats. Conclusion This study thus concludes that M. charantia nanoparticles may provide effective therapeutics against mitochondria dysfunction in the pancreas of diabetic model.Olusola Olalekan ElekofehintiOlusola Christianah AyodeleOpeyemi IwaloyeSpringerOpenarticleDiabetes mellitusMitochondria biogenesisInflammationGlucose sensitivityMomordica charantia nanoparticlesMedicine (General)R5-920GeneticsQH426-470ENEgyptian Journal of Medical Human Genetics, Vol 22, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Diabetes mellitus Mitochondria biogenesis Inflammation Glucose sensitivity Momordica charantia nanoparticles Medicine (General) R5-920 Genetics QH426-470
spellingShingle	Diabetes mellitus Mitochondria biogenesis Inflammation Glucose sensitivity Momordica charantia nanoparticles Medicine (General) R5-920 Genetics QH426-470 Olusola Olalekan Elekofehinti Olusola Christianah Ayodele Opeyemi Iwaloye Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study
description	Abstract Background Mitochondria dysfunction is one of the clinical features of diabetes mellitus (DM), which is a hallmark of insulin resistance (IR). This study investigates the therapeutic effect of Momordica charantia nanoparticles on mitochondria biogenesis in diabetic-induced rats. Forty-two adult wistar rats (average weight of 189 ± 10.32) were grouped as follows: STZ (65 mg/kg), control group, STZ + silver nitrate (10 mg/kg), STZ + M. charantia silver nanoparticles (50 mg/kg), STZ + metformin (100 mg/kg), and STZ + M. charantia aqueous extract (100 mg/kg). DM was induced intraperitoneal using freshly prepared solution of STZ (65 mg/kg), and rats with fasting blood sugar (FBS) above 250 mg/dl after 72 h of induction were considered diabetic. Treatment started after the third day of induction and lasted for 11 days. Effect of M. charantia nanoparticles on glucose level and pancreatic expression of genes involved in mitochondria biogenesis (PGC-1α, AMPK, GSK-3β, PPARϒ), inflammation (IL-1B, TNFα) and glucose sensitivity (PI3K, AKT, PTEN Insulin and Glut2) were quantified using reverse-transcriptase polymerase chain reaction (RT-PCR). Results The results showed that M. charantia nanoparticles promote mitochondria biogenesis, glucose sensitivity and reverse inflammation in the pancreas of diabetes rat model through upregulation of PGC-1α, AMPK, PPARϒ, AKT, Insulin and Glut2 mRNA expression and downregulation of GSK-3β, PI3K, IL-1B and TNFα mRNA expression in the pancreas of diabetic rats. Conclusion This study thus concludes that M. charantia nanoparticles may provide effective therapeutics against mitochondria dysfunction in the pancreas of diabetic model.
format	article
author	Olusola Olalekan Elekofehinti Olusola Christianah Ayodele Opeyemi Iwaloye
author_facet	Olusola Olalekan Elekofehinti Olusola Christianah Ayodele Opeyemi Iwaloye
author_sort	Olusola Olalekan Elekofehinti
title	Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study
title_short	Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study
title_full	Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study
title_fullStr	Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study
title_full_unstemmed	Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study
title_sort	momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study
publisher	SpringerOpen
publishDate	2021
url	https://doaj.org/article/fcf74983362a4fa8bbc674abd6799b99
work_keys_str_mv	AT olusolaolalekanelekofehinti momordicacharantiananoparticlespromotemitochondriabiogenesisinthepancreasofdiabeticinducedratsgeneexpressionstudy AT olusolachristianahayodele momordicacharantiananoparticlespromotemitochondriabiogenesisinthepancreasofdiabeticinducedratsgeneexpressionstudy AT opeyemiiwaloye momordicacharantiananoparticlespromotemitochondriabiogenesisinthepancreasofdiabeticinducedratsgeneexpressionstudy
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Momordica charantia nanoparticles promote mitochondria biogenesis in the pancreas of diabetic-induced rats: gene expression study

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