Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4

Abstract Mantle cell lymphoma (MCL) is a malignancy arising from naive B lymphocytes with common bone marrow (BM) involvement. Although t(11;14) is a primary event in MCL development, the highly diverse molecular etiology and causal genomic events are still being explored. We investigated the transc...

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Autores principales:	Simone Valentin Hansen, Marcus Høy Hansen, Oriane Cédile, Michael Boe Møller, Jacob Haaber, Niels Abildgaard, Charlotte Guldborg Nyvold
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fcfd47c3dcaf495b817c3a62de0ec18d
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spelling	oai:doaj.org-article:fcfd47c3dcaf495b817c3a62de0ec18d2021-12-02T18:51:28ZDetailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX410.1038/s41598-021-98560-12045-2322https://doaj.org/article/fcfd47c3dcaf495b817c3a62de0ec18d2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98560-1https://doaj.org/toc/2045-2322Abstract Mantle cell lymphoma (MCL) is a malignancy arising from naive B lymphocytes with common bone marrow (BM) involvement. Although t(11;14) is a primary event in MCL development, the highly diverse molecular etiology and causal genomic events are still being explored. We investigated the transcriptome of CD19+ BM cells from eight MCL patients at single-cell level. The transcriptomes revealed marked heterogeneity across patients, while general homogeneity and clonal continuity was observed within the patients with no clear evidence of subclonal involvement. All patients were SOX11+CCND1+CD20+. Despite monotypic surface immunoglobulin (Ig) κ or λ protein expression in MCL, 10.9% of the SOX11 + malignant cells expressed both light chain transcripts. The early lymphocyte transcription factor SOX4 was expressed in a fraction of SOX11 + cells in two patients and co-expressed with the precursor lymphoblastic marker, FAT1, in a blastoid case, suggesting a potential prognostic role. Additionally, SOX4 was found to identify non-malignant SOX11– pro-/pre-B cell populations. Altogether, the observed expression of markers such as SOX4, CD27, IgA and IgG in the SOX11+ MCL cells, may suggest that the malignant cells are not fixed in the differentiation state of naïve mature B cells, but instead the patients carry B lymphocytes of different differentiation stages.Simone Valentin HansenMarcus Høy HansenOriane CédileMichael Boe MøllerJacob HaaberNiels AbildgaardCharlotte Guldborg NyvoldNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Valentin Hansen Marcus Høy Hansen Oriane Cédile Michael Boe Møller Jacob Haaber Niels Abildgaard Charlotte Guldborg Nyvold Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4
description	Abstract Mantle cell lymphoma (MCL) is a malignancy arising from naive B lymphocytes with common bone marrow (BM) involvement. Although t(11;14) is a primary event in MCL development, the highly diverse molecular etiology and causal genomic events are still being explored. We investigated the transcriptome of CD19+ BM cells from eight MCL patients at single-cell level. The transcriptomes revealed marked heterogeneity across patients, while general homogeneity and clonal continuity was observed within the patients with no clear evidence of subclonal involvement. All patients were SOX11+CCND1+CD20+. Despite monotypic surface immunoglobulin (Ig) κ or λ protein expression in MCL, 10.9% of the SOX11 + malignant cells expressed both light chain transcripts. The early lymphocyte transcription factor SOX4 was expressed in a fraction of SOX11 + cells in two patients and co-expressed with the precursor lymphoblastic marker, FAT1, in a blastoid case, suggesting a potential prognostic role. Additionally, SOX4 was found to identify non-malignant SOX11– pro-/pre-B cell populations. Altogether, the observed expression of markers such as SOX4, CD27, IgA and IgG in the SOX11+ MCL cells, may suggest that the malignant cells are not fixed in the differentiation state of naïve mature B cells, but instead the patients carry B lymphocytes of different differentiation stages.
format	article
author	Simone Valentin Hansen Marcus Høy Hansen Oriane Cédile Michael Boe Møller Jacob Haaber Niels Abildgaard Charlotte Guldborg Nyvold
author_facet	Simone Valentin Hansen Marcus Høy Hansen Oriane Cédile Michael Boe Møller Jacob Haaber Niels Abildgaard Charlotte Guldborg Nyvold
author_sort	Simone Valentin Hansen
title	Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4
title_short	Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4
title_full	Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4
title_fullStr	Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4
title_full_unstemmed	Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4
title_sort	detailed characterization of the transcriptome of single b cells in mantle cell lymphoma suggesting a potential use for sox4
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/fcfd47c3dcaf495b817c3a62de0ec18d
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Detailed characterization of the transcriptome of single B cells in mantle cell lymphoma suggesting a potential use for SOX4

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