Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo

Endotoxin shock in rats led to intravenous bolus of bacterial lipopolysaccharide (LPS) was accompanied by oxidative stress and increase of NO level in livers, lungs, kidneys, hearts of animals. The intravenous administration of bienzyme superoxide dismutase - chondroitin sulphate - catalase conjugat...

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Autores principales:	A. V. Maksimenko, A. V. Vavaeva, A. A. Abramov, A. A. Timoshin, M. A. Zvyagintseva, V. L. Lakomkin
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	no-зависимое и no-независимое защитное действие lipopolysaccharide endotoxin shock oxidative stress antioxidants bienzyme superoxide dismutase-chondroitin sulfate-catalase conjugate Science Q
Acceso en línea:	https://doaj.org/article/fd034d646a3243bb9fed0df9cd2a7cbc
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spelling	oai:doaj.org-article:fd034d646a3243bb9fed0df9cd2a7cbc2021-11-23T06:14:34ZProtective action of bienzyme conjugate of antioxidant biocatalysts in vivo2541-94202587-959610.12737/article_590823a4d95e23.38896065https://doaj.org/article/fd034d646a3243bb9fed0df9cd2a7cbc2016-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/201https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Endotoxin shock in rats led to intravenous bolus of bacterial lipopolysaccharide (LPS) was accompanied by oxidative stress and increase of NO level in livers, lungs, kidneys, hearts of animals. The intravenous administration of bienzyme superoxide dismutase - chondroitin sulphate - catalase conjugate (SOD-CHS-CAT) showed its anti-endotoxemia action in rats at preventive (before initiation of injury) and therapeutic (after start of injury) application during cytokine phase of injury and influence on further stages of the damage. The injection of antioxidant SOD-CHS-CAT conjugate in a therapeutic regime did not demonstrate significant alterations of No level in livers, lungs, kidneys, hearts of rats (as compared to indices of the rat group with LpS action only) and displayed protective action on the kidney function by altered urea and creatinine in blood. The therapeutic effect of SOD-CHS-CAT was demonstrated by increase in organism viability. The effects of intravenous injection of SOD-CHS-CAT in a rat model of endotoxin shock showed a variety in the activity of this conjugate in addition to prevention of NO conversion in peroxynitrite upon interaction with O2 superoxide radical. The results of accelerated normalization of arterial blood pressure and heart rate, a significant decrease in rat lethality because of using SOD-CHS-CAT conjugate indicate the importance of the determination of action mechanism of the cardiovascular injury models associated with other vasoactive agents besides NO. The further research of vascular damage pathogenesis and evaluation of its role in oxidative stress (time and place) are of paramount importance.A. V. MaksimenkoA. V. VavaevaA. A. AbramovA. A. TimoshinM. A. ZvyagintsevaV. L. LakomkinScientific Сentre for Family Health and Human Reproduction Problemsarticleno-зависимое и no-независимое защитное действиеlipopolysaccharideendotoxin shockoxidative stressantioxidantsbienzyme superoxide dismutase-chondroitin sulfate-catalase conjugateScienceQRUActa Biomedica Scientifica, Vol 1, Iss 3(2), Pp 116-119 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	no-зависимое и no-независимое защитное действие lipopolysaccharide endotoxin shock oxidative stress antioxidants bienzyme superoxide dismutase-chondroitin sulfate-catalase conjugate Science Q
spellingShingle	no-зависимое и no-независимое защитное действие lipopolysaccharide endotoxin shock oxidative stress antioxidants bienzyme superoxide dismutase-chondroitin sulfate-catalase conjugate Science Q A. V. Maksimenko A. V. Vavaeva A. A. Abramov A. A. Timoshin M. A. Zvyagintseva V. L. Lakomkin Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo
description	Endotoxin shock in rats led to intravenous bolus of bacterial lipopolysaccharide (LPS) was accompanied by oxidative stress and increase of NO level in livers, lungs, kidneys, hearts of animals. The intravenous administration of bienzyme superoxide dismutase - chondroitin sulphate - catalase conjugate (SOD-CHS-CAT) showed its anti-endotoxemia action in rats at preventive (before initiation of injury) and therapeutic (after start of injury) application during cytokine phase of injury and influence on further stages of the damage. The injection of antioxidant SOD-CHS-CAT conjugate in a therapeutic regime did not demonstrate significant alterations of No level in livers, lungs, kidneys, hearts of rats (as compared to indices of the rat group with LpS action only) and displayed protective action on the kidney function by altered urea and creatinine in blood. The therapeutic effect of SOD-CHS-CAT was demonstrated by increase in organism viability. The effects of intravenous injection of SOD-CHS-CAT in a rat model of endotoxin shock showed a variety in the activity of this conjugate in addition to prevention of NO conversion in peroxynitrite upon interaction with O2 superoxide radical. The results of accelerated normalization of arterial blood pressure and heart rate, a significant decrease in rat lethality because of using SOD-CHS-CAT conjugate indicate the importance of the determination of action mechanism of the cardiovascular injury models associated with other vasoactive agents besides NO. The further research of vascular damage pathogenesis and evaluation of its role in oxidative stress (time and place) are of paramount importance.
format	article
author	A. V. Maksimenko A. V. Vavaeva A. A. Abramov A. A. Timoshin M. A. Zvyagintseva V. L. Lakomkin
author_facet	A. V. Maksimenko A. V. Vavaeva A. A. Abramov A. A. Timoshin M. A. Zvyagintseva V. L. Lakomkin
author_sort	A. V. Maksimenko
title	Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo
title_short	Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo
title_full	Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo
title_fullStr	Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo
title_full_unstemmed	Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo
title_sort	protective action of bienzyme conjugate of antioxidant biocatalysts in vivo
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/fd034d646a3243bb9fed0df9cd2a7cbc
work_keys_str_mv	AT avmaksimenko protectiveactionofbienzymeconjugateofantioxidantbiocatalystsinvivo AT avvavaeva protectiveactionofbienzymeconjugateofantioxidantbiocatalystsinvivo AT aaabramov protectiveactionofbienzymeconjugateofantioxidantbiocatalystsinvivo AT aatimoshin protectiveactionofbienzymeconjugateofantioxidantbiocatalystsinvivo AT mazvyagintseva protectiveactionofbienzymeconjugateofantioxidantbiocatalystsinvivo AT vllakomkin protectiveactionofbienzymeconjugateofantioxidantbiocatalystsinvivo
_version_	1718417110425665536

Protective action of bienzyme conjugate of antioxidant biocatalysts in vivo

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