SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION

Search for novel prognostic criteria predicting successful in vitro fertilization remains a nonresolved problem at the present time. The aim of our study was to analyse a predictive role of IL-1α as an additional marker of pregnancy after in vitro fertilization (IVF). The study included 120 women wi...

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Autores principales:	A. V. Lapshtaeva, I. V. Evsegneeva, V. V. Novikov, I. V. Sychev, A. V. Karaulov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	il-1α tubo-peritoneal infertility pregnancy in vitro fertilization Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/fd05f2880c3348378138ae59c59f7952
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spelling	oai:doaj.org-article:fd05f2880c3348378138ae59c59f79522021-11-18T08:03:47ZSERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION1563-06252313-741X10.15789/1563-0625-2018-1-115-122https://doaj.org/article/fd05f2880c3348378138ae59c59f79522018-01-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1438https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XSearch for novel prognostic criteria predicting successful in vitro fertilization remains a nonresolved problem at the present time. The aim of our study was to analyse a predictive role of IL-1α as an additional marker of pregnancy after in vitro fertilization (IVF). The study included 120 women with tubo-peritoneal infertility subjected to the IVF procedure. Retrospectively, two groups were formed of this cohort, dependent on efficiency of in vitro fertilization. Group I included 40 women with successful pregnancy whereas group II comprised 80 women with failed pregnancy. IL-1α concentrations in serum were detected by ELISA technique. A polymorphic rs1800587 marker at 5`UTR region has been amplified by PCR followed by Sanger sequencing. We have shown IL-1α hyperproduction in the women from group I. The women with effective IVF outcome exhibited positive correlation between IL-1α and luteinizing hormone, prolactin, progesterone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate levels. The women with ineffective in vitro fertilization have detected a negative correlation between IL-1α levels and anti-Muellerian hormone, a positive correlation of IL-1α with 17-hydroxyprogesterone. The women with T allele of the polymorphic rs1800587 marker at 5`UTR region have shown a 2.5-fold higher chance to become pregnant after IVF than the women carrying C allele (95% CI = [1.45-4.35], р = 0.0009). The women with T/T genotype exhibited a positive correlation between IL-1α and estradiol, testosterone; the subjects with heterozygous C/T genotype showed correlation with estradiol, and those harboring C/C genotype exhibited correlation with follicle-stimulating hormone. The revealed changes suggest a potential involvement of IL-1α into regulation of cyclic processes in the ovary including ovulation. Moreover, IL-1α may participate in formation of pro-inflammatory environment for successful blastocyst implantation.A. V. LapshtaevaI. V. EvsegneevaV. V. NovikovI. V. SychevA. V. KaraulovSPb RAACIarticleil-1αtubo-peritoneal infertilitypregnancyin vitro fertilizationImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 1, Pp 115-122 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	il-1α tubo-peritoneal infertility pregnancy in vitro fertilization Immunologic diseases. Allergy RC581-607
spellingShingle	il-1α tubo-peritoneal infertility pregnancy in vitro fertilization Immunologic diseases. Allergy RC581-607 A. V. Lapshtaeva I. V. Evsegneeva V. V. Novikov I. V. Sychev A. V. Karaulov SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION
description	Search for novel prognostic criteria predicting successful in vitro fertilization remains a nonresolved problem at the present time. The aim of our study was to analyse a predictive role of IL-1α as an additional marker of pregnancy after in vitro fertilization (IVF). The study included 120 women with tubo-peritoneal infertility subjected to the IVF procedure. Retrospectively, two groups were formed of this cohort, dependent on efficiency of in vitro fertilization. Group I included 40 women with successful pregnancy whereas group II comprised 80 women with failed pregnancy. IL-1α concentrations in serum were detected by ELISA technique. A polymorphic rs1800587 marker at 5`UTR region has been amplified by PCR followed by Sanger sequencing. We have shown IL-1α hyperproduction in the women from group I. The women with effective IVF outcome exhibited positive correlation between IL-1α and luteinizing hormone, prolactin, progesterone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate levels. The women with ineffective in vitro fertilization have detected a negative correlation between IL-1α levels and anti-Muellerian hormone, a positive correlation of IL-1α with 17-hydroxyprogesterone. The women with T allele of the polymorphic rs1800587 marker at 5`UTR region have shown a 2.5-fold higher chance to become pregnant after IVF than the women carrying C allele (95% CI = [1.45-4.35], р = 0.0009). The women with T/T genotype exhibited a positive correlation between IL-1α and estradiol, testosterone; the subjects with heterozygous C/T genotype showed correlation with estradiol, and those harboring C/C genotype exhibited correlation with follicle-stimulating hormone. The revealed changes suggest a potential involvement of IL-1α into regulation of cyclic processes in the ovary including ovulation. Moreover, IL-1α may participate in formation of pro-inflammatory environment for successful blastocyst implantation.
format	article
author	A. V. Lapshtaeva I. V. Evsegneeva V. V. Novikov I. V. Sychev A. V. Karaulov
author_facet	A. V. Lapshtaeva I. V. Evsegneeva V. V. Novikov I. V. Sychev A. V. Karaulov
author_sort	A. V. Lapshtaeva
title	SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION
title_short	SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION
title_full	SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION
title_fullStr	SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION
title_full_unstemmed	SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION
title_sort	serum level and gene polymorphism of interleukin-1α, and efficiency of infertility treatment by in vitro fertilization
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/fd05f2880c3348378138ae59c59f7952
work_keys_str_mv	AT avlapshtaeva serumlevelandgenepolymorphismofinterleukin1aandefficiencyofinfertilitytreatmentbyinvitrofertilization AT ivevsegneeva serumlevelandgenepolymorphismofinterleukin1aandefficiencyofinfertilitytreatmentbyinvitrofertilization AT vvnovikov serumlevelandgenepolymorphismofinterleukin1aandefficiencyofinfertilitytreatmentbyinvitrofertilization AT ivsychev serumlevelandgenepolymorphismofinterleukin1aandefficiencyofinfertilitytreatmentbyinvitrofertilization AT avkaraulov serumlevelandgenepolymorphismofinterleukin1aandefficiencyofinfertilitytreatmentbyinvitrofertilization
_version_	1718422392871583744

SERUM LEVEL AND GENE POLYMORPHISM OF INTERLEUKIN-1α, AND EFFICIENCY OF INFERTILITY TREATMENT BY IN VITRO FERTILIZATION

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