Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).

Highly specialized obligate plant-parasites exist within several groups of arthropods (insects and mites). Many of these are important pests, but the molecular basis of their parasitism and its evolution are poorly understood. One hypothesis is that plant parasitic arthropods use effector proteins t...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rajat Aggarwal, Subhashree Subramanyam, Chaoyang Zhao, Ming-Shun Chen, Marion O Harris, Jeff J Stuart
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/fd0641efb2f0438fb9d2927ba8ee4112
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:fd0641efb2f0438fb9d2927ba8ee4112
record_format	dspace
spelling	oai:doaj.org-article:fd0641efb2f0438fb9d2927ba8ee41122021-11-11T08:21:35ZAvirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).1932-620310.1371/journal.pone.0100958https://doaj.org/article/fd0641efb2f0438fb9d2927ba8ee41122014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24964065/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Highly specialized obligate plant-parasites exist within several groups of arthropods (insects and mites). Many of these are important pests, but the molecular basis of their parasitism and its evolution are poorly understood. One hypothesis is that plant parasitic arthropods use effector proteins to defeat basal plant immunity and modulate plant growth. Because avirulence (Avr) gene discovery is a reliable method of effector identification, we tested this hypothesis using high-resolution molecular genetic mapping of an Avr gene (vH13) in the Hessian fly (HF, Mayetiola destructor), an important gall midge pest of wheat (Triticum spp.). Chromosome walking resolved the position of vH13, and revealed alleles that determine whether HF larvae are virulent (survive) or avirulent (die) on wheat seedlings carrying the wheat H13 resistance gene. Association mapping found three independent insertions in vH13 that appear to be responsible for H13-virulence in field populations. We observed vH13 transcription in H13-avirulent larvae and the salivary glands of H13-avirulent larvae, but not in H13-virulent larvae. RNA-interference-knockdown of vH13 transcripts allowed some H13-avirulent larvae to escape H13-directed resistance. vH13 is the first Avr gene identified in an arthropod. It encodes a small modular protein with no sequence similarities to other proteins in GenBank. These data clearly support the hypothesis that an effector-based strategy has evolved in multiple lineages of plant parasites, including arthropods.Rajat AggarwalSubhashree SubramanyamChaoyang ZhaoMing-Shun ChenMarion O HarrisJeff J StuartPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100958 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rajat Aggarwal Subhashree Subramanyam Chaoyang Zhao Ming-Shun Chen Marion O Harris Jeff J Stuart Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).
description	Highly specialized obligate plant-parasites exist within several groups of arthropods (insects and mites). Many of these are important pests, but the molecular basis of their parasitism and its evolution are poorly understood. One hypothesis is that plant parasitic arthropods use effector proteins to defeat basal plant immunity and modulate plant growth. Because avirulence (Avr) gene discovery is a reliable method of effector identification, we tested this hypothesis using high-resolution molecular genetic mapping of an Avr gene (vH13) in the Hessian fly (HF, Mayetiola destructor), an important gall midge pest of wheat (Triticum spp.). Chromosome walking resolved the position of vH13, and revealed alleles that determine whether HF larvae are virulent (survive) or avirulent (die) on wheat seedlings carrying the wheat H13 resistance gene. Association mapping found three independent insertions in vH13 that appear to be responsible for H13-virulence in field populations. We observed vH13 transcription in H13-avirulent larvae and the salivary glands of H13-avirulent larvae, but not in H13-virulent larvae. RNA-interference-knockdown of vH13 transcripts allowed some H13-avirulent larvae to escape H13-directed resistance. vH13 is the first Avr gene identified in an arthropod. It encodes a small modular protein with no sequence similarities to other proteins in GenBank. These data clearly support the hypothesis that an effector-based strategy has evolved in multiple lineages of plant parasites, including arthropods.
format	article
author	Rajat Aggarwal Subhashree Subramanyam Chaoyang Zhao Ming-Shun Chen Marion O Harris Jeff J Stuart
author_facet	Rajat Aggarwal Subhashree Subramanyam Chaoyang Zhao Ming-Shun Chen Marion O Harris Jeff J Stuart
author_sort	Rajat Aggarwal
title	Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).
title_short	Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).
title_full	Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).
title_fullStr	Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).
title_full_unstemmed	Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).
title_sort	avirulence effector discovery in a plant galling and plant parasitic arthropod, the hessian fly (mayetiola destructor).
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/fd0641efb2f0438fb9d2927ba8ee4112
work_keys_str_mv	AT rajataggarwal avirulenceeffectordiscoveryinaplantgallingandplantparasiticarthropodthehessianflymayetioladestructor AT subhashreesubramanyam avirulenceeffectordiscoveryinaplantgallingandplantparasiticarthropodthehessianflymayetioladestructor AT chaoyangzhao avirulenceeffectordiscoveryinaplantgallingandplantparasiticarthropodthehessianflymayetioladestructor AT mingshunchen avirulenceeffectordiscoveryinaplantgallingandplantparasiticarthropodthehessianflymayetioladestructor AT marionoharris avirulenceeffectordiscoveryinaplantgallingandplantparasiticarthropodthehessianflymayetioladestructor AT jeffjstuart avirulenceeffectordiscoveryinaplantgallingandplantparasiticarthropodthehessianflymayetioladestructor
_version_	1718439367667613696

Avirulence effector discovery in a plant galling and plant parasitic arthropod, the Hessian fly (Mayetiola destructor).

Ejemplares similares