Membrane Vesicles of Pectobacterium as an Effective Protein Secretion System

Bacteria of genus Pectobacterium are Gram-negative rods of the family Pectobacteriaceae. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesicles (MVs) a...

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Autores principales:	Joanna Jonca, Malgorzata Waleron, Paulina Czaplewska, Aleksandra Bogucka, Aleksandra Steć, Szymon Dziomba, Jacek Jasiecki, Michał Rychłowski, Krzysztof Waleron
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacterial membrane vesicles <i>Pectobacterium</i> proteomic analysis plant pathogens bacterial virulence Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fd071ad2e63644eba96f8c0e6c1b7aed
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spelling	oai:doaj.org-article:fd071ad2e63644eba96f8c0e6c1b7aed2021-11-25T17:58:02ZMembrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System10.3390/ijms2222125741422-00671661-6596https://doaj.org/article/fd071ad2e63644eba96f8c0e6c1b7aed2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12574https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Bacteria of genus <i>Pectobacterium</i> are Gram-negative rods of the family <i>Pectobacteriaceae</i>. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesicles (MVs) are universally released by bacteria and are believed to play an important role in the pathogenicity and survival of bacteria in the environment. Our study investigates the role of MVs in the virulence of <i>Pectobacterium</i>. The results indicate that the morphology and MVs production depend on growth medium composition. In polygalacturonic acid (PGA) supplemented media, <i>Pectobacterium</i> produces large MVs (100–300 nm) and small vesicles below 100 nm. Proteomic analyses revealed the presence of pectate degrading enzymes in the MVs. The pectate plate test and enzymatic assay proved that those enzymes are active and able to degrade pectates. What is more, the pathogenicity test indicated that the MVs derived from <i>Pectobacterium</i> were able to induce maceration of <i>Zantedeschia</i> sp. leaves. We also show that the MVs of β-lactamase producing strains were able to suppress ampicillin activity and permit the growth of susceptible bacteria. Those findings indicate that the MVs of <i>Pectobacterium</i> play an important role in host-pathogen interactions and niche competition with other bacteria. Our research also sheds some light on the mechanism of MVs production. We demonstrate that the MVs production in <i>Pectobacterium</i> strains, which overexpress a green fluorescence protein (GFP), is higher than in wild-type strains. Moreover, proteomic analysis revealed that the GFP was present in the MVs. Therefore, it is possible that protein sequestration into MVs might not be strictly limited to periplasmic proteins. Our research highlights the importance of MVs production as a mechanism of cargo delivery in <i>Pectobacterium</i> and an effective secretion system.Joanna JoncaMalgorzata WaleronPaulina CzaplewskaAleksandra BoguckaAleksandra StećSzymon DziombaJacek JasieckiMichał RychłowskiKrzysztof WaleronMDPI AGarticlebacterial membrane vesicles<i>Pectobacterium</i>proteomic analysisplant pathogensbacterial virulenceBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12574, p 12574 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacterial membrane vesicles <i>Pectobacterium</i> proteomic analysis plant pathogens bacterial virulence Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	bacterial membrane vesicles <i>Pectobacterium</i> proteomic analysis plant pathogens bacterial virulence Biology (General) QH301-705.5 Chemistry QD1-999 Joanna Jonca Malgorzata Waleron Paulina Czaplewska Aleksandra Bogucka Aleksandra Steć Szymon Dziomba Jacek Jasiecki Michał Rychłowski Krzysztof Waleron Membrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System
description	Bacteria of genus <i>Pectobacterium</i> are Gram-negative rods of the family <i>Pectobacteriaceae</i>. They are the causative agent of soft rot diseases of crops and ornamental plants. However, their virulence mechanisms are not yet fully elucidated. Membrane vesicles (MVs) are universally released by bacteria and are believed to play an important role in the pathogenicity and survival of bacteria in the environment. Our study investigates the role of MVs in the virulence of <i>Pectobacterium</i>. The results indicate that the morphology and MVs production depend on growth medium composition. In polygalacturonic acid (PGA) supplemented media, <i>Pectobacterium</i> produces large MVs (100–300 nm) and small vesicles below 100 nm. Proteomic analyses revealed the presence of pectate degrading enzymes in the MVs. The pectate plate test and enzymatic assay proved that those enzymes are active and able to degrade pectates. What is more, the pathogenicity test indicated that the MVs derived from <i>Pectobacterium</i> were able to induce maceration of <i>Zantedeschia</i> sp. leaves. We also show that the MVs of β-lactamase producing strains were able to suppress ampicillin activity and permit the growth of susceptible bacteria. Those findings indicate that the MVs of <i>Pectobacterium</i> play an important role in host-pathogen interactions and niche competition with other bacteria. Our research also sheds some light on the mechanism of MVs production. We demonstrate that the MVs production in <i>Pectobacterium</i> strains, which overexpress a green fluorescence protein (GFP), is higher than in wild-type strains. Moreover, proteomic analysis revealed that the GFP was present in the MVs. Therefore, it is possible that protein sequestration into MVs might not be strictly limited to periplasmic proteins. Our research highlights the importance of MVs production as a mechanism of cargo delivery in <i>Pectobacterium</i> and an effective secretion system.
format	article
author	Joanna Jonca Malgorzata Waleron Paulina Czaplewska Aleksandra Bogucka Aleksandra Steć Szymon Dziomba Jacek Jasiecki Michał Rychłowski Krzysztof Waleron
author_facet	Joanna Jonca Malgorzata Waleron Paulina Czaplewska Aleksandra Bogucka Aleksandra Steć Szymon Dziomba Jacek Jasiecki Michał Rychłowski Krzysztof Waleron
author_sort	Joanna Jonca
title	Membrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System
title_short	Membrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System
title_full	Membrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System
title_fullStr	Membrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System
title_full_unstemmed	Membrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System
title_sort	membrane vesicles of <i>pectobacterium</i> as an effective protein secretion system
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fd071ad2e63644eba96f8c0e6c1b7aed
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Membrane Vesicles of <i>Pectobacterium</i> as an Effective Protein Secretion System

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