MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds

Soybeans are nutritionally important as human food and animal feed. Apart from the macronutrients such as proteins and oils, soybeans are also high in health-beneficial secondary metabolites and are uniquely enriched in isoflavones among food crops. Isoflavone biosynthesis has been relatively well c...

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Autores principales:	Ming-Sin Ng, Yee-Shan Ku, Wai-Shing Yung, Sau-Shan Cheng, Chun-Kuen Man, Liu Yang, Shikui Song, Gyuhwa Chung, Hon-Ming Lam
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	soybean seed isoflavone multidrug and toxic compound extrusion (MATE) transporter genistein daidzein Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/fd07f3d16b1b489184d4ccf5fd91bcb9
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spelling	oai:doaj.org-article:fd07f3d16b1b489184d4ccf5fd91bcb92021-11-11T17:25:40ZMATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds10.3390/ijms2221120171422-00671661-6596https://doaj.org/article/fd07f3d16b1b489184d4ccf5fd91bcb92021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12017https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Soybeans are nutritionally important as human food and animal feed. Apart from the macronutrients such as proteins and oils, soybeans are also high in health-beneficial secondary metabolites and are uniquely enriched in isoflavones among food crops. Isoflavone biosynthesis has been relatively well characterized, but the mechanism of their transportation in soybean cells is largely unknown. Using the yeast model, we showed that GmMATE1 and GmMATE2 promoted the accumulation of isoflavones, mainly in the aglycone forms. Using the tobacco BrightYellow-2 (BY-2) cell model, GmMATE1 and GmMATE2 were found to be localized in the vacuolar membrane. Such subcellular localization supports the notion that GmMATE1 and GmMATE2 function by compartmentalizing isoflavones in the vacuole. Expression analyses showed that GmMATE1 was mainly expressed in the developing soybean pod. Soybean mutants defective in GmMATE1 had significantly reduced total seed isoflavone contents, whereas the overexpression of GmMATE1 in transgenic soybean promoted the accumulation of seed isoflavones. Our results showed that GmMATE1, and possibly also GmMATE2, are <i>bona fide</i> isoflavone transporters that promote the accumulation of isoflavones in soybean seeds.Ming-Sin NgYee-Shan KuWai-Shing YungSau-Shan ChengChun-Kuen ManLiu YangShikui SongGyuhwa ChungHon-Ming LamMDPI AGarticlesoybeanseedisoflavonemultidrug and toxic compound extrusion (MATE) transportergenisteindaidzeinBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12017, p 12017 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	soybean seed isoflavone multidrug and toxic compound extrusion (MATE) transporter genistein daidzein Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	soybean seed isoflavone multidrug and toxic compound extrusion (MATE) transporter genistein daidzein Biology (General) QH301-705.5 Chemistry QD1-999 Ming-Sin Ng Yee-Shan Ku Wai-Shing Yung Sau-Shan Cheng Chun-Kuen Man Liu Yang Shikui Song Gyuhwa Chung Hon-Ming Lam MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds
description	Soybeans are nutritionally important as human food and animal feed. Apart from the macronutrients such as proteins and oils, soybeans are also high in health-beneficial secondary metabolites and are uniquely enriched in isoflavones among food crops. Isoflavone biosynthesis has been relatively well characterized, but the mechanism of their transportation in soybean cells is largely unknown. Using the yeast model, we showed that GmMATE1 and GmMATE2 promoted the accumulation of isoflavones, mainly in the aglycone forms. Using the tobacco BrightYellow-2 (BY-2) cell model, GmMATE1 and GmMATE2 were found to be localized in the vacuolar membrane. Such subcellular localization supports the notion that GmMATE1 and GmMATE2 function by compartmentalizing isoflavones in the vacuole. Expression analyses showed that GmMATE1 was mainly expressed in the developing soybean pod. Soybean mutants defective in GmMATE1 had significantly reduced total seed isoflavone contents, whereas the overexpression of GmMATE1 in transgenic soybean promoted the accumulation of seed isoflavones. Our results showed that GmMATE1, and possibly also GmMATE2, are <i>bona fide</i> isoflavone transporters that promote the accumulation of isoflavones in soybean seeds.
format	article
author	Ming-Sin Ng Yee-Shan Ku Wai-Shing Yung Sau-Shan Cheng Chun-Kuen Man Liu Yang Shikui Song Gyuhwa Chung Hon-Ming Lam
author_facet	Ming-Sin Ng Yee-Shan Ku Wai-Shing Yung Sau-Shan Cheng Chun-Kuen Man Liu Yang Shikui Song Gyuhwa Chung Hon-Ming Lam
author_sort	Ming-Sin Ng
title	MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds
title_short	MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds
title_full	MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds
title_fullStr	MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds
title_full_unstemmed	MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds
title_sort	mate-type proteins are responsible for isoflavone transportation and accumulation in soybean seeds
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/fd07f3d16b1b489184d4ccf5fd91bcb9
work_keys_str_mv	AT mingsinng matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT yeeshanku matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT waishingyung matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT saushancheng matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT chunkuenman matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT liuyang matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT shikuisong matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT gyuhwachung matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds AT honminglam matetypeproteinsareresponsibleforisoflavonetransportationandaccumulationinsoybeanseeds
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MATE-Type Proteins Are Responsible for Isoflavone Transportation and Accumulation in Soybean Seeds

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