Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation

The composition of gut-associated microbial communities changes during intestinal inflammation, including an expansion of Enterobacteriaceae populations. The mechanisms underlying microbiota changes during inflammation are incompletely understood. Here, we analyzed previously published metagenomic d...

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Autores principales:	Elizabeth R Hughes, Maria G Winter, Laice Alves da Silva, Matthew K Muramatsu, Angel G Jimenez, Caroline C Gillis, Luisella Spiga, Rachael B Chanin, Renato L Santos, Wenhan Zhu, Sebastian E Winter
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	gut microbiota dysbiosis intestinal inflammation hydrogenase molecular hydrogen Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/fd14f6f2f03b419ba41300000a9ffb71
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spelling	oai:doaj.org-article:fd14f6f2f03b419ba41300000a9ffb712021-11-11T09:31:34ZReshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation10.7554/eLife.586092050-084Xe58609https://doaj.org/article/fd14f6f2f03b419ba41300000a9ffb712021-06-01T00:00:00Zhttps://elifesciences.org/articles/58609https://doaj.org/toc/2050-084XThe composition of gut-associated microbial communities changes during intestinal inflammation, including an expansion of Enterobacteriaceae populations. The mechanisms underlying microbiota changes during inflammation are incompletely understood. Here, we analyzed previously published metagenomic datasets with a focus on microbial hydrogen metabolism. The bacterial genomes in the inflamed murine gut and in patients with inflammatory bowel disease contained more genes encoding predicted hydrogen-utilizing hydrogenases compared to communities found under non-inflamed conditions. To validate these findings, we investigated hydrogen metabolism of Escherichia coli, a representative Enterobacteriaceae, in mouse models of colitis. E. coli mutants lacking hydrogenase-1 and hydrogenase-2 displayed decreased fitness during colonization of the inflamed cecum and colon. Utilization of molecular hydrogen was in part dependent on respiration of inflammation-derived electron acceptors. This work highlights the contribution of hydrogenases to alterations of the gut microbiota in the context of non-infectious colitis.Elizabeth R HughesMaria G WinterLaice Alves da SilvaMatthew K MuramatsuAngel G JimenezCaroline C GillisLuisella SpigaRachael B ChaninRenato L SantosWenhan ZhuSebastian E WintereLife Sciences Publications Ltdarticlegut microbiotadysbiosisintestinal inflammationhydrogenasemolecular hydrogenMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gut microbiota dysbiosis intestinal inflammation hydrogenase molecular hydrogen Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	gut microbiota dysbiosis intestinal inflammation hydrogenase molecular hydrogen Medicine R Science Q Biology (General) QH301-705.5 Elizabeth R Hughes Maria G Winter Laice Alves da Silva Matthew K Muramatsu Angel G Jimenez Caroline C Gillis Luisella Spiga Rachael B Chanin Renato L Santos Wenhan Zhu Sebastian E Winter Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation
description	The composition of gut-associated microbial communities changes during intestinal inflammation, including an expansion of Enterobacteriaceae populations. The mechanisms underlying microbiota changes during inflammation are incompletely understood. Here, we analyzed previously published metagenomic datasets with a focus on microbial hydrogen metabolism. The bacterial genomes in the inflamed murine gut and in patients with inflammatory bowel disease contained more genes encoding predicted hydrogen-utilizing hydrogenases compared to communities found under non-inflamed conditions. To validate these findings, we investigated hydrogen metabolism of Escherichia coli, a representative Enterobacteriaceae, in mouse models of colitis. E. coli mutants lacking hydrogenase-1 and hydrogenase-2 displayed decreased fitness during colonization of the inflamed cecum and colon. Utilization of molecular hydrogen was in part dependent on respiration of inflammation-derived electron acceptors. This work highlights the contribution of hydrogenases to alterations of the gut microbiota in the context of non-infectious colitis.
format	article
author	Elizabeth R Hughes Maria G Winter Laice Alves da Silva Matthew K Muramatsu Angel G Jimenez Caroline C Gillis Luisella Spiga Rachael B Chanin Renato L Santos Wenhan Zhu Sebastian E Winter
author_facet	Elizabeth R Hughes Maria G Winter Laice Alves da Silva Matthew K Muramatsu Angel G Jimenez Caroline C Gillis Luisella Spiga Rachael B Chanin Renato L Santos Wenhan Zhu Sebastian E Winter
author_sort	Elizabeth R Hughes
title	Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation
title_short	Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation
title_full	Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation
title_fullStr	Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation
title_full_unstemmed	Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation
title_sort	reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal e. coli during gut inflammation
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/fd14f6f2f03b419ba41300000a9ffb71
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Reshaping of bacterial molecular hydrogen metabolism contributes to the outgrowth of commensal E. coli during gut inflammation

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