Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier

Yasutaka Mori1,3,4, Shingo Nakamura2, Satoko Kishimoto1, Mitsuyuki Kawakami4, Satoshi Suzuki4, Takemi Matsui4, Masayuki Ishihara11Research Institute, 2Department of Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan; 3Aeromedical Laboratory, Japan Air Self-Defense Force, Sayama, S...

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Autores principales:	Yasutaka Mori, Shingo Nakamura, Satoko Kishimoto, et al
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/fd16341a8df241ad99b294d80fed92bc
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spelling	oai:doaj.org-article:fd16341a8df241ad99b294d80fed92bc2021-12-02T07:47:00ZPreparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier1176-91141178-2013https://doaj.org/article/fd16341a8df241ad99b294d80fed92bc2010-03-01T00:00:00Zhttp://www.dovepress.com/preparation-and-characterization-of-low-molecular-weight-heparinprotam-a4078https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yasutaka Mori1,3,4, Shingo Nakamura2, Satoko Kishimoto1, Mitsuyuki Kawakami4, Satoshi Suzuki4, Takemi Matsui4, Masayuki Ishihara11Research Institute, 2Department of Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan; 3Aeromedical Laboratory, Japan Air Self-Defense Force, Sayama, Saitama, Japan; 4Faculty of System Design, Tokyo Metropolitan University, Hino, Tokyo, JapanAbstract: We produced low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as a carrier for heparin-binding growth factors, such as fibroblast growth factor-2 (FGF-2). A mixture of low-molecular-weight heparin (MW: about 5000 Da, 6.4 mg/mL) and protamine (MW: about 3000 Da, 10 mg/mL) at a ratio of 7:3 (vol:vol) yields a dispersion of microparticles (1&ndash;6 &mu;m in diameter). In this study, diluted low-molecular-weight heparin solution in saline (0.32 mg/mL) mixed with diluted protamine (0.5 mg/mL) at a ratio at 7:3 (vol:vol) resulted in soluble nanoparticles (112.5 &plusmn; 46.1 nm in diameter). The generated NPs could be then stabilized by adding 2 mg/mL dextran (MW: 178-217 kDa) and remained soluble after lyophilization of dialyzed LMW-H/P NP solution. We then evaluated the capacity of LMW-H/P NPs to protect activity of FGF-2. Interaction between FGF-2 and LMW-H/P NPs substantially prolonged the biological half-life of FGF-2. Furthermore, FGF-2 molecules were protected from inactivation by heat and proteolysis in the presence of LMW-H/P NPs.Keywords: polyelectrolyte complexes, nanoparticles, fibroblast growth factor-2, drug carrier Yasutaka MoriShingo NakamuraSatoko Kishimotoet alDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2010, Iss default, Pp 147-155 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Yasutaka Mori Shingo Nakamura Satoko Kishimoto et al Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier
description	Yasutaka Mori1,3,4, Shingo Nakamura2, Satoko Kishimoto1, Mitsuyuki Kawakami4, Satoshi Suzuki4, Takemi Matsui4, Masayuki Ishihara11Research Institute, 2Department of Surgery, National Defense Medical College, Tokorozawa, Saitama, Japan; 3Aeromedical Laboratory, Japan Air Self-Defense Force, Sayama, Saitama, Japan; 4Faculty of System Design, Tokyo Metropolitan University, Hino, Tokyo, JapanAbstract: We produced low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as a carrier for heparin-binding growth factors, such as fibroblast growth factor-2 (FGF-2). A mixture of low-molecular-weight heparin (MW: about 5000 Da, 6.4 mg/mL) and protamine (MW: about 3000 Da, 10 mg/mL) at a ratio of 7:3 (vol:vol) yields a dispersion of microparticles (1&ndash;6 &mu;m in diameter). In this study, diluted low-molecular-weight heparin solution in saline (0.32 mg/mL) mixed with diluted protamine (0.5 mg/mL) at a ratio at 7:3 (vol:vol) resulted in soluble nanoparticles (112.5 &plusmn; 46.1 nm in diameter). The generated NPs could be then stabilized by adding 2 mg/mL dextran (MW: 178-217 kDa) and remained soluble after lyophilization of dialyzed LMW-H/P NP solution. We then evaluated the capacity of LMW-H/P NPs to protect activity of FGF-2. Interaction between FGF-2 and LMW-H/P NPs substantially prolonged the biological half-life of FGF-2. Furthermore, FGF-2 molecules were protected from inactivation by heat and proteolysis in the presence of LMW-H/P NPs.Keywords: polyelectrolyte complexes, nanoparticles, fibroblast growth factor-2, drug carrier
format	article
author	Yasutaka Mori Shingo Nakamura Satoko Kishimoto et al
author_facet	Yasutaka Mori Shingo Nakamura Satoko Kishimoto et al
author_sort	Yasutaka Mori
title	Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier
title_short	Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier
title_full	Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier
title_fullStr	Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier
title_full_unstemmed	Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier
title_sort	preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (lmw-h/p nps) as fgf-2 carrier
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/fd16341a8df241ad99b294d80fed92bc
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Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier

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